Protein GmZF 392 derived from soybeans and applications of related biomaterials in regulation and control of plant fat
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A plant and protein technology, applied in the application field of regulating vegetable oils, can solve problems such as little known
Active Publication Date: 2014-11-19
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
View PDF4 Cites 8 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, in plants, the regulatory mechanism of lipid synthesis and its related genes are still poorly understood
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0067]Example 1, cDNA cloning of the GmZF392 encoding gene related to regulation of soybean lipid metabolism and construction of plant expression vector
[0068] 1. Cloning of protein GmZF392 gene and construction of plant expression vector
[0069] Total RNA was extracted from Heinong 44 seedlings, and the RNA was reverse-transcribed with reverse transcriptase to synthesize cDNA.
[0070] According to the information of GmZF392 full-length cDNA sequence in the soybean genome sequence of PlantGDB, design primers, the primer sequences are as follows:
[0071] GmZF392-up: 5'-ATGAGCAGTGTCTGTGCCA-3';
[0072] GmZF392-dp: 5'-TTACATCAGCAACTCGTTCACC-3'.
[0073] Using HN44cDNA as a template and using GmZF392-up and GmZF392-dp as primers, PCR amplification was performed to obtain a PCR product of about 1Kb. After sequencing, the PCR product is 1179bp, its nucleotide sequence is SEQ ID No.1, the DNA molecule shown in the nucleotide sequence is the GmZF392 gene (GmZF392), the protein...
Embodiment 2
[0082] Embodiment 2, the acquisition of transgenic GmZF392 Arabidopsis
[0083] 1. Obtaining recombinant Agrobacterium
[0084] The recombinant vector pGWB412-GmZF392 containing GmZF392 obtained in Example 1 was introduced into Agrobacterium tumefaciens GV3101 by electroporation to obtain recombinant Agrobacterium GV3101 / GmZF392 containing pGWB412-GmZF392.
[0085] 2. Obtaining and identification of transgenic GmZF392 Arabidopsis
[0086] The recombinant Agrobacterium GV3101 / GmZF392 was cultivated to the logarithmic phase, and then transformed into Arabidopsis thaliana (col-0) by vacuum method. The seeds were purchased from Arabidopsis Biological Resource Center (ABRC). Seeds harvested after cultivation (T 1 generation), the seeds were sown on the MS selection medium containing kanamycin (50mg / L), and the T 1 When the generation plant grows to 4-6 leaves, it is moved to the vermiculite to grow, and the harvest T 1 The seeds of each individual plant were sown separately, an...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a protein GmZF 392 derived from soybeans and applications of related biomaterials in regulation and control of plant fat. One application is a method for cultivation of transgenic seed plants with a high total fat content and/or a high fatty acid content. The method comprises steps: a GmZF 392 gene is introduced into an acceptor seed plant and a transgenic seed plant with a total fat content being higher than the total fat content of the acceptor seed plant and/or a fatty acid content being higher than the total fatty acid content of the acceptor seed plant is obtained. The fatty acids are at least one of the followings: linolenic acid, linoleic acid, oleic acid, a fatty acid named after gongduosuan, sinapic acid, palmitic acid and stearic acid. The GmZF 392 encoding gene has important theoretical and practical significances to raise and improve crop fat components, especially to raise fat components in seeds of oil plants of soybeans and the like and to cultivate varieties with high fat.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to the application of protein GmZF392 derived from soybean and related biological materials in regulating vegetable oil. Background technique [0002] 71% of the fat in the human diet comes from plants. Among several major oil-producing crops in the world, the total oil production of soybean accounts for about 30%, ranking first in the world's vegetable oil production (Table 1). [0003] Table 1. Major oil-producing crops in the world [0004] [0005] The synthesis of fatty acids is one of the most important metabolic pathways in plants. It exists in any cell of plants and is necessary for growth and development. Blocking it leads to cell death, so a plant mutant that blocks fatty acid synthesis has not been found so far. [0006] Plants differ greatly from other eukaryotes in the enzymes involved in the fatty acid synthesis pathway. Synthesis of fatty acids with 16 or 18 carbon...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00
Inventor 陈受宜张劲松李擎天宋庆鑫张万科马彪林晴何锶洁
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
Login to view more 
Login to view more