Detection of adenosine by an aptamer sensor based on colocalization-triggered chain hybridization

Abstract

The invention discloses a colocalization trigger chain type hybridization reaction based aptamer sensor for detecting adenosine. The colocalization trigger chain type hybridization reaction based aptamer sensor consists of an aptamer chain 1, an aptamer chain 2 and a hairpin probe which are biotinylated and a magnetic nano-sphere modified by streptavidin. The aptamer sensor is used for detecting adenosine, and a detection method comprises the following steps: (1) activation of a streptavidin modified magnetic sphere; (2) fixation of the biotinylated aptamer chain 1; (3) colocalization of adenosine-induced double aptamers; (4) colocalization trigger chain type hybridization reaction; (5) addition of a dye SYBR Green I and fluorescence detection; (6) calculation: calculating the concentration of adenosine in a to-be-detected object containing adenosine according to a measured fluorescence value. The aptamer sensor disclosed by the invention can be used for reducing the non-specific reaction of conventional chain type hybridization reaction by virtue of the colocalization-induced hybridization reaction, has the advantages of high detection sensitivity and less sample usage without enzyme amplification, and can achieve the analysis and detection of low-concentration adenosine.

Description

technical field [0001] The invention relates to the construction of an adenosine aptamer sensor based on colocalization triggered chain hybridization reaction to detect adenosine. Background technique [0002] Adenosine is an important biopharmaceutical small molecule, which participates in the regulation of various life activities of the body, and has important physiological and pharmacological effects, such as relaxing blood vessels, contracting smooth muscles, and regulating the nervous system and immune system. In addition, the detection of adenosine in biological samples can be used for disease diagnosis, monitoring of disease course and early diagnosis of cancer. Therefore, the quantitative detection of adenosine, especially the detection of adenosine in biological samples, has important biological significance in clinical research, disease diagnosis and prevention, and early diagnosis of cancer. [0003] Although a large number of adenosine aptamer sensing systems ha...

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Problems solved by technology

[0005] Aiming at the problem that the existing adenosine analysis technology cannot realize the direct detection of adenosine in biological samples, the purpose of the present invention is to provide an aptamer sensor based on colocalization to trigger chain hybridization reaction, and to use the aptamer sensor for adenosine The analysis and detection of glycosides has the advantages of high sensitivity, strong selectivity and good reproducibility, and can be directly used for the determination of adenosine in human urine without any pretreatment process

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