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Application of eupatorium sesquiterpene component in preparing drug for resisting acute lung injury

A technology for acute lung injury and sesquiterpene, which is applied to the application field of wild horse sesquiterpene parts in the preparation of anti-acute lung injury medicines

Active Publication Date: 2014-11-26
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on whether these five sesquiterpene compounds have anti-acute lung injury effects

Method used

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  • Application of eupatorium sesquiterpene component in preparing drug for resisting acute lung injury
  • Application of eupatorium sesquiterpene component in preparing drug for resisting acute lung injury
  • Application of eupatorium sesquiterpene component in preparing drug for resisting acute lung injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Preparation of Sesquiterpene Fraction from Yemazhui and Detection of Active Components

[0044] (1) Preparation of the sesquiterpene part of Yemazhui:

[0045] Sample 1: Weigh 300g of Yemazhui dried whole herb, cut into 2-3cm sections, add 10 times the volume of 80% ethanol aqueous solution, reflux extraction for 2 hours, repeat twice, recover the solvent under reduced pressure at 40°C to obtain Yemazhui Ethanol extract 45g; Dissolve this ethanol extract in about 90ml of water, absorb through 500ml of D101 macroporous adsorption resin, remove macromolecular compound with 5000ml water elution first, be 95% ethanol aqueous solution elution with 5000ml volume fraction behind, reduce The solvent was recovered under pressure to obtain 17g of macroporous resin; the obtained macroporous resin was adsorbed by 350ml of YMCODS-A reversed-phase silica gel filler particle size 60um reversed-phase silica gel column, eluted with 1750ml volume fraction of 50% methanol aqueous solution...

Embodiment 2

[0058] Anti-acute lung injury experiment of Yemazhui sesquiterpene fraction (EUP-SQT) in mice

[0059] 1. Experimental drugs and reagents: 0.5% sodium carboxymethylcellulose; lipopolysaccharide (LPS), Sigma-Aldrich, USA; dexamethasone acetate tablets, Zhejiang Xianju Pharmaceutical Co., Ltd.; polyclonal rabbit anti-human C3c complement, Shanghai Ruiqi Biotechnology Co., Ltd.; distilled water; normal saline; 20% urethane-normal saline; 4% formalin, H-E staining solution, 3,3'-diaminobenzidine (DAB) chromogenic solution, etc.

[0060] 2. Experimental mice: Balb / c mice, male, weighing 24-28 grams, provided by the Experimental Animal Center of Soochow University, experimental animal production license: XCYK (Su) 2002-0008. The experimental animals were raised in an environment with a temperature of 24±1° C. and a relative humidity of 40% to 80%, with free access to food and water. Before the experiment, they were adaptively fed for 7 days.

[0061] 3. Other materials: mouse tumo...

Embodiment 3

[0081] Comparative experiment on anti-acute lung injury effect of Yemazhui sesquiterpene part and Yemazhui ethanol extract

[0082] Preparation of Yemazhui ethanol extract: Weigh the dried whole herb of Yemazhui, cut it into sections of 2-3 cm, add 10 times the volume of 80% ethanol aqueous solution, reflux extraction for 2 hours, repeat twice, recover the solvent under reduced pressure, and obtain Yemazhui ethanol Extract (EtOH).

[0083] In this comparative experiment, the experimental reagents, experimental mice and experimental materials are the same as those in Example 2.

[0084] Experimental mice were randomly divided into 5 groups including blank group, model group, EtOH group of ethanol extract of Yemazhui, EUP-SQT group of sesquiterpene part of Yemazhui and positive group, 10 mice in each group. Yemazhui ethanol extract EtOH group was given EtOH at a dose of 30 mg·kg -1 ; Yemazhui sesquiterpene part SQT group was given EUP-SQT, the dose was 30mg·kg -1 ; The positi...

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Abstract

The invention discloses an application of eupatorium sesquiterpene components in preparing a drug for resisting acute lung injury. Eupatorium sesquiterpene components are made into a veterinary drug. A technical solution provided by the invention comprises the steps of making the eupatorium sesquiterpene componentsinto a veterinary drug; inducing acute lung injury in mice by using lipopolysaccharide (LPS) in a manner of in-vivo dosing to the mice; then determining a lung wet / dry ratio of the mice with the acute lung injury, the content of nitric oxide (NO) and protein in a bronchoalveolar lavage fluid (BALF); detecting activities of myeloperoxidase (MPO) and superoxide dismutase (SOD) in lung tissue homogenate, the content of tumor necrosis factor-alpha (TN-alpha), interleukin-6 (IL-6) and interleukin-1[beta] (IL-1[beta]) in the BALF and the content of complement C3 and C3c in blood serum; and observing pathological changes of lung tissues of the mice after H&E staining. Results show that eupatorium sesquiterpene components of eupalinolide F, eupalinolide G, eupalinolide H, eupalinolide I and eupalinolide K have activity for resisting the acute lung injury.

Description

technical field [0001] The invention relates to the application of sesquiterpene parts of Yemachai in the preparation of anti-acute lung injury medicine. Background technique [0002] Acute lung injury (ALI) is based on diffuse lung cell injury, pulmonary edema and pulmonary inflammatory cell infiltration caused by pulmonary vascular injury are its pathological features, and the main clinical manifestation is severe hypoxemia , diffuse pulmonary infiltration and pulmonary edema; some patients will eventually develop acute respiratory distress syndrome (ARDS), resulting in irreversible acute respiratory failure and multiple organ dysfunction, with a fatality rate as high as 30-40% The pathogenesis of ALI is complex, and the risk factors for ALI can be direct damage from the lung, or indirect damage to the lung caused by extrapulmonary factors through systemic inflammatory response. (See: Baffert F, Le T, Thurston G, et al. Angiopoietin-1 decreases plasma leakage by reducing ...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P11/00A61K31/365
Inventor 张健褚纯隽任慧玲陈道峰严彪李显伦吴天威徐乃玉
Owner SUZHOU UNIV
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