Housefly larvae active protein peptide preparation method and housefly larvae active protein peptide prepared thereby and purpose of housefly larvae active protein peptide

A technology of active protein and fly maggots, which is applied in the fields of medicine, pharmacy, and protein biochemistry, can solve the problems of poor treatment cycle, cost consumption, and patient loss, and achieve the effects of low production cost, high-efficiency preparation, and simple operation

Active Publication Date: 2014-11-26
吉林金梓源生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The medicine prepared by using the denatured fly maggot active protein peptide must have poor treatment effect and poor treatment cycle, which will bring more pain and loss of medicine expenses to patients
Bring more cost consumption in production

Method used

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  • Housefly larvae active protein peptide preparation method and housefly larvae active protein peptide prepared thereby and purpose of housefly larvae active protein peptide
  • Housefly larvae active protein peptide preparation method and housefly larvae active protein peptide prepared thereby and purpose of housefly larvae active protein peptide
  • Housefly larvae active protein peptide preparation method and housefly larvae active protein peptide prepared thereby and purpose of housefly larvae active protein peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] For the live and fresh fly maggots, use a sieve with appropriate aperture to remove impurities as much as possible, put them into woven bags, and store them in a freezer at -20°C.

[0087] During preparation, take 1 kg of freshly frozen fly maggots, add 5 liters of deionized water, stir and thaw, use colloid mill to refine, adjust pH to 7.2, centrifuge at 14000 rpm (=12000g), take clear liquid, and use 60,000 channels The filter membrane with a molecular weight cut-off of 400-500 Daltons is used for ultrafiltration, and the passing liquid is concentrated to 1 liter with a nanofiltration membrane between 400-500 Daltons, and then deionized water is added to 5 liters, and the liquid is concentrated with a nanofiltration membrane between 400-500 Daltons. Concentrate with nanofiltration membrane, wash the desalted protein peptide repeatedly 4 times, finally concentrate to 0.3 liters, sterilize with a sterilizing filter, and freeze-dry to obtain 0.05 kg of fly maggot active p...

Embodiment 2

[0089] Similar to the method in Example 2, for the fresh and live fly maggots raised, use a basket sieve with an appropriate aperture to remove impurities as much as possible, put them into woven bags, and store them frozen at -20°C;

[0090] Take 5 kg of freshly frozen fly maggots, add 25 liters of deionized water, stir and thaw, use a colloid mill to refine the pulp, adjust the pH to 7.2, and use a centrifuge at 14,000 rpm to take the clarified liquid and use a filter membrane with a molecular weight of 60,000 Daltons Ultrafiltration, concentrate the passing liquid to 5 liters with a nanofiltration membrane between 400-500 Daltons, then add deionized water to 25 liters, stir and concentrate by nanofiltration, repeat 4 times, finally concentrate to 1.5 liters, freeze Dried to obtain 0.25 kg of fly maggot active protein peptide.

Embodiment 3

[0092] Similar to the method in Example 2, for the fresh and live fly maggots raised, use a basket sieve with an appropriate aperture to remove impurities as much as possible, put them into woven bags, and store them frozen at -20°C;

[0093] Take 10kg of freshly frozen fly maggots, add 50 liters of deionized water, stir and thaw, use a colloid mill to refine the pulp, adjust the pH to 7.2, 14000 rpm / centrifuge, take the clarified liquid, and use a filter membrane with a molecular weight of 60,000 Daltons Ultrafiltration, concentrate the passing liquid to 10 liters with a nanofiltration machine, then add deionized water to 50 liters, stir and concentrate with nanofiltration, repeat 4 times, finally concentrate to 2.5 liters, sterilize with a sterilizing filter, freeze-dry , get 0.5 kilogram of fly maggot active protein peptide.

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Abstract

The invention provides a housefly larvae active protein peptide preparation method which includes the following steps: freezing of fresh housefly larvae, thawing, grinding, pH adjusting, extracting, centrifugal separation, ultrafiltration, nanofiltration desalination, concentration, filtration sterilization, freeze drying, product subpacking. The method has the advantages of simple operation and low production cost, is suitable for large-scale industrialized production, and can realize the purpose of efficient preparation of protein peptide from the housefly larvae. The invention also relates to housefly larvae active protein peptide obtained by the method, and the housefly larvae active protein peptide has significant skin regenerative repair functional activity to postburn patients. The invention also relates to the purpose of the housefly larvae active protein peptide obtained by the method, the use includes application of the housefly larvae active protein peptide in preparation of medicines, food, health care products or cosmetics for prevention and / or treatment of human traumatic skin disease.

Description

technical field [0001] The invention relates to a method for preparing highly active protein peptides from fly maggots, the prepared products and uses thereof, belonging to the fields of protein biochemistry, medicine and pharmacy. Background technique [0002] The housefly (Musca demestica) is an animal of the genus Musca (Musca). It is a complete metamorphosis insect, and its life cycle includes four stages: egg, larva (maggot), pupa, and adult (fly, Fly maggot). Fly maggots are ubiquitous in nature, have complete antibacterial, reproductive and damage repair mechanisms, and have strong environmental adaptability. This environmental adaptation mechanism is acquired by houseflies in the long-term evolution process. Studies have shown that fly maggots contain powerful defense mechanisms, such as antimicrobial peptides, peroxidase and metallothionein for anti-oxidation, and epidermal growth factor for self-repair. These functional components of fly maggots provide valuable ...

Claims

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Application Information

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IPC IPC(8): C07K1/34C07K1/14A23L1/29A61K8/64A61K38/17A61P17/02A61Q19/00A61Q7/00A23L33/00
Inventor 刘爽庞海庞玉邓小霞
Owner 吉林金梓源生物科技股份有限公司
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