Method using acetic acid for controlled catalysis of orientational hydrolysis of xylan to prepare multicomponent xylooligosaccharide

A technology of xylooligosaccharides and catalytic wood, which is applied in the fields of food engineering and chemical engineering, can solve the problems of high requirements for the production of enzyme preparations, and achieve the effects of overcoming many toxic decomposition products, simple process, and high yield cost

Active Publication Date: 2014-11-26
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods must rely on the catalysis of endo-xylanase with highly selective catalysis, which has high requirements for the production of enzyme preparations.

Method used

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  • Method using acetic acid for controlled catalysis of orientational hydrolysis of xylan to prepare multicomponent xylooligosaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] In a 1L mechanically stirred stainless steel high-pressure reaction tank bottle, add 700mL of reactants containing 10% (mass percentage, the same below) xylan and 20% (mass percentage, the same below) edible acetic acid, seal it, and turn on the stirring speed of 150r / min, heated to 120°C for 2 hours, and quickly cooled the reaction system to room temperature in a water bath to obtain a reaction solution.

[0018] The components of the reaction solution were detected by high-performance liquid chromatography, and the chromatographic conditions were: American Dionex ICS-3000 ion chromatography system, equipped with CarboPac TM PA200 (3mm×250mm) chromatographic column and retention column (3×50mm), ED electrochemical detector, column temperature 30°C, injection volume 10.0μL; 100mmol / L sodium hydroxide-500mmol / L sodium acetate solution (A ) and 100mmol / L sodium hydroxide solution (B) as the eluent for binary gradient elution at a flow rate of 0.3mL / min (elution program,...

Embodiment 2

[0021] Add 600mL reactants containing 20% ​​xylan and 30% edible acetic acid to a 1L mechanically stirred stainless steel high-pressure reaction tank bottle, seal it, turn on the stirring speed at 160r / min, heat to 130°C for 1.5h, and quickly cool in a water bath The reaction system was brought to room temperature to obtain a reaction liquid. A microfiltration membrane device is used to separate the reaction liquid, intercept unhydrolyzed xylan and pass through the xylooligosaccharide liquid. Then, the nanofiltration membrane device is used to separate the reaction solution by batch replenishment, retain more than 95% of the xylo-oligosaccharide component, pass through more than 98% of acetic acid and more than 92% of xylose, and obtain the xylo-oligosaccharide product liquid. The acetic acid solution was reused. The composition of the reaction solution was detected by high performance liquid phase ion chromatography, the method was the same as in Example 1, the results showe...

Embodiment 3

[0023] Add 550mL reactants containing 15% xylan and 15% edible acetic acid to a 1L mechanically stirred stainless steel high-pressure reaction tank bottle, seal it, turn on the stirring speed of 150r / min, heat to 110°C for 3 hours, and quickly cool the reaction in a water bath The system was brought to room temperature to obtain a reaction solution. A microfiltration membrane device is used to separate the reaction liquid, intercept unhydrolyzed xylan and pass through the xylooligosaccharide liquid. Then, the nanofiltration membrane device is used to separate the reaction solution by batch replenishment, retain more than 95% of the xylo-oligosaccharide component, pass through more than 98% of acetic acid and more than 92% of xylose, and obtain the xylo-oligosaccharide product liquid. The acetic acid solution was reused. High-performance liquid phase ion chromatography was used to detect the components of the reaction solution. The method was the same as in Example 1. Formic a...

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Abstract

The present invention discloses a method using acetic acid for controlled catalysis of orientational hydrolysis of xylan to prepare multicomponent xylooligosaccharide, and the method includes the following steps: 1) adding food grade acetic acid to a suspension reaction system containing no more than 20% of the xylan until the final concentration of the acetic acid is not more than 30%, controlling the reaction temperature at 110 to 125 DEG C for reaction for no more than 3h to obtain a xylan hydrolysate; 2) performing microfiltration and nanofiltration on the xylan hydrolysate product to obtain an entrapped xylooligosaccharide solution and an acetic acid through liquid, and recycling the acetic acid through liquid for using in the step 1); and 3) using an edible alkaline material for neutralizing the entrapped xylooligosaccharide solution to neutral and producing sodium acetate, and removing the sodium acetate by electrodialysis treatment to obtain a xylooligosaccharide product solution. The recyclable edible acetic acid is used as a catalyst for the realization of controlled hydrolysis reaction of the xylan in the mild reaction conditions for orientational catalysis of hydrolysis of the xylan to prepare a xylooligosaccharide product containing xylooligosaccharide components with the polymerization degree of 2-8, the yield is more than 30% of raw material xylooligosaccharide, the product almost contains no furan, formic acid and other decomposition products, and xylooligosaccharide green production can be achieved.

Description

technical field [0001] The invention relates to the fields of food engineering and chemical engineering, in particular to a method for preparing multi-component xylooligosaccharides by directional hydrolysis of xylan catalyzed by acetic acid. Background technique [0002] As a functional oligosaccharide, xylooligosaccharide can be widely used in food, medicine and feed industries, and is an important way for high-value conversion and utilization of plant xylan. At present, the production of xylo-oligosaccharides mainly uses endo-xylanase preparations to catalyze the hydrolysis of natural xylan in plant raw materials or xylan extracted by alkali; or use weak acid to cook at 155-180°C for 30-120min The xylan in the corn cob is dissolved, and then hydrolyzed by xylanase to produce xylooligosaccharides (CN200410023875; CN 01131171.1). The above-mentioned methods must rely on the catalysis of endo-xylanase with highly selective catalysis, and have high requirements for the produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C13K13/00C08B37/00
Inventor 徐勇张红玉余世袁勇强蒋发现周鑫
Owner NANJING FORESTRY UNIV
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