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Intervertebral disc tissue scaffold including growth factor and preparation method thereof

A tissue scaffold and growth factor technology, applied in medical science, prosthesis, etc., can solve the problems of poor mechanical properties of hydrogel, easy cell death, and limited use, and achieve simple and easy preparation methods, good uniformity, and good The effect of mechanical properties

Inactive Publication Date: 2014-12-10
THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies mostly used hydrogel as a carrier for carrying growth factors, but its disadvantages include easy death of cells inside the hydrogel, poor mechanical properties of the hydrogel, and limited use.

Method used

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  • Intervertebral disc tissue scaffold including growth factor and preparation method thereof
  • Intervertebral disc tissue scaffold including growth factor and preparation method thereof
  • Intervertebral disc tissue scaffold including growth factor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 adopts the method of the present invention to prepare the same intervertebral disc tissue support

[0028] Step 1. Dissolve polylactic acid-glycolic acid copolymer (PLGA) in hexafluoroisopropanol (HFIP) to make a 15% PLGA solution, which is the outer layer solution of coaxial electrospinning;

[0029] Step 2. Dilute TGF-β1 (Prospec Company) with 1% bovine serum albumin (BSA) to a concentration of 1 ng / μL;

[0030] Step 3. Dissolve polyvinyl acetate (PVA) in ultrapure water to form a 15% PVA solution.

[0031] Step 4. Add 6 μL of 1 ng / μL TGF-β1 to 2 mL of 15% PVA solution, which is the inner layer solution of coaxial electrospinning;

[0032] Step 5, such as figure 1 Assemble the instrument, the syringe is (LSP02-1B, Baoding Lange Company, China), the high-voltage power supply is an intermediate frequency DC high voltage device (RXZGF, Shanghai Rixing Company, China), the distance between the needle and the receiving device is 15cm, the voltage is 16-18Kv,...

Embodiment 2

[0034] Embodiment 2 detects the performance of the intervertebral disc tissue support of the present invention

[0035] 1. Observing the constructed intervertebral disc tissue scaffold with a scanning electron microscope, and counting the distribution of spinning diameters. see results figure 2 . Scanning electron microscope results show that the spinning diameter is relatively uniform, and most of them are distributed in the range of 200-500nm. The double-layer structure of the intervertebral disc tissue scaffold can be clearly seen by the transmission electron microscope.

[0036] 2. Use a transmission electron microscope to observe the spinning shell-nucleus double-layer structure of the intervertebral disc tissue scaffold. The results are shown in image 3 . A clear boundary can be seen between the inner core layer and the outer shell layer, indicating that the spinning of inner and outer double shell-core structures was successfully obtained.

[0037] 3. In order to...

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Abstract

The invention belongs to the technical field of tissue engineering, particularly relates to an intervertebral disc tissue scaffold including agrowth factor and a preparation method thereof, and solves the technical problem by providing a tissue engineering scaffold for treating low back pain. The invention adopts the technical scheme that the intervertebral disc tissue scaffold including a growth factor is a coaxial electrospinning scaffold prepared by adopting the coaxial electrospinning technique, and the growth factor is TGF-beta 1. The invention further provides a preparation method of the intervertebral disc tissue scaffold. The intervertebral disc tissue scaffold can be used for treating low back pain.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to a growth factor-containing intervertebral disc tissue support and a preparation method thereof. Background technique [0002] Low back pain is a common clinical disease that seriously affects people's quality of life. Studies have shown that intervertebral disc degeneration and nucleus pulposus calcification are closely related to low back pain. Conservative treatment can only relieve symptoms. Although discectomy or spinal segmental fusion and internal fixation can relieve nerve compression symptoms, there is a high incidence of intervertebral joint stiffness and recurrence of intervertebral disc herniation after surgery. At present, biological treatment methods include gene therapy, growth factor therapy, cell and tissue engineering, etc. [0003] Tissue engineering is a method of combining cells and scaffolds to replace damaged parts. However, there a...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/22A61L27/54A61L27/50
Inventor 崔翔刘铭汉王加旭黄博向强周跃
Owner THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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