To provide a separation and discrimination method of a
nucleus pulposus
precursor cell, and application thereof.SOLUTION: Provided is a separation and discrimination method of a
nucleus pulposus
precursor cell, where a large number of tissue blocks are divided from a
nucleus pulposus tissue, and the tissue blocks are left to stand and cultured in a culture dish after performing
hydrolysis by
enzyme. When cells are formed into the partition blocks on a bottom of the culture dish, a nucleus pulposus
precursor cell is screened from the partition blocks by using a specific
embryonic stem cell gene (e.g., Nanog, Oct-4, SOX2). Further, the method can screen a nucleus pulposus precursor
cell having moving performance and / or
mesenchymal stem cell properties by using a
specific protein and a
gene marker, and further can screen a nucleus pulposus precursor
cell having anti-inflammatory performance. Finally, disclosed is application used for producing a pharmaceutical composition treating lower
back pain by using the nucleus pulposus precursor
cell obtained by the method, and a new therapy strategy to lower
back pain is provided.SELECTED DRAWING: Figure 6