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Preparation method of S-adenosyl methionline p-toluenesulfonate sulfate

A technology of adenosylmethionine and p-toluenesulfonic acid, which is applied to the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of increased separation and purification costs, long process flow, and difficult recovery, and reduce production The effect of cost reduction, environmental pollution reduction and simple operation

Inactive Publication Date: 2014-12-17
北京东方瑞德生物技术有限公司
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Problems solved by technology

[0008] (2) There are two-step precipitation processes in the preparation of the stable salt, and the technological process is long, and three kinds of organic solvents such as acetone, methyl alcohol, and ethyl acetate are used, which will increase the cost of separation and purification on the one hand; difficulty

Method used

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  • Preparation method of S-adenosyl methionline p-toluenesulfonate sulfate

Examples

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example 1

[0023] The preparation of example 1 S-adenosylmethionine p-toluenesulfonic acid sulfate

[0024] 1) After the fermentation of Saccharomyces cerevisiae, centrifuge at 4000 rpm for 10 minutes, and collect 2.6 kg of wet bacteria. Add 3.9 liters of 0.35 mol / L oxalic acid solution, stir for 10 minutes, and then flow through a high-pressure homogenizer with the pressure maintained at 1200 bar. Centrifuge at 8000 rpm for 20 minutes to remove cell debris to obtain 4.7 liters of extract with a SAM content of 11.7 g / L.

[0025] 2) Adjust the pH value of the SAM extract to 5.0 with 5mol / L sodium hydroxide, dilute to 8g / L with pure water, and pass through the 724 resin column at a flow rate of 2BV / h. After loading the sample, the column was washed with 6 times the volume of deionized water, and then eluted with 0.15 mol / L sulfuric acid to obtain 3 liters of SAM eluent with a concentration of 16.5 g / L.

[0026] 3) Add the strong base resin D201 of hydroxide type to the eluent to make the...

example 2

[0030] The preparation of example 2 S-adenosylmethionine p-toluenesulfonic acid sulfate

[0031] 1) After the fermentation of Saccharomyces cerevisiae, centrifuge at 4000 rpm for 10 minutes, and collect 3.0 kg of wet bacteria. Add 3.6 liters of 0.4 mol / L oxalic acid solution, stir for 10 minutes, then flow through a high-pressure homogenizer, maintain the pressure at 900 bar, and homogenize twice. Centrifuge at 8000 rpm for 20 minutes to remove cell debris and obtain 4.5 liters of extract with a SAM content of 13.3 g / L.

[0032] 2) Adjust the pH value of the SAM extract to 6.0 with 5mol / L sodium hydroxide, dilute to 8g / L with pure water, and pass through the 724 resin column at a flow rate of 1.5BV / h. After loading the sample, wash the column with 5BV of pure water, and then elute with 0.1mol / L sulfuric acid to obtain 3.3 liters of SAM eluent with a concentration of 15.2g / L.

[0033] 3) Add 201×4 hydroxide-type strong base resin to the eluent to make the pH reach 4.0, vacuum...

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Abstract

The invention relates to a preparation method of S-adenosyl methionline p-toluenesulfonate sulfate. According to the method, S-adenosyl methionline in yeast cells is firstly released by the use of an oxalic acid solution and with the combination of high pressure homogenization; then, purification and salt-forming of S-adenosyl methionline are realized through two resins; and finally, a product is obtained through vacuum concentration and spray drying. The technology is simple; no organic solvent is used; and cost is low. The preparation method is easy for industrial production.

Description

[0001] Technical field: the present invention relates to a kind of preparation method of S-adenosylmethionine p-toluenesulfonic acid sulfate Background technique: [0002] S-adenosylmethionine, also known as S-adenosylmethionine (S-adenosylmethionine in English, abbreviated as SAM, SAMe or AdeMet), is an important metabolic intermediate widely present in animals, plants and microorganisms. As an important physiologically active substance in the human body, it has participated in more than 40 biochemical reactions, mainly playing the role of transmethylation, transsulfurization, and transaminopropylation. It is semi-sarcosine, taurine, glutathione, Precursor of important substances such as coenzyme A. In the metabolic network of organisms, the biosynthesis of SAM is completed by the reaction of SAM synthetase [EC2.5.1.6] catalyzing methionine (Met) and adenosine triphosphate (ATP). As a key metabolic intermediate in the human body, SAM has a variety of important physiological ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H19/167C07H1/06
Inventor 宋奇健
Owner 北京东方瑞德生物技术有限公司
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