Method for extracting piceatannol from fresh passion fruit seeds

A technology of piceatannol and passionflower, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry and other directions, can solve the problems of pollution, low purity of piceatannol, low extraction efficiency and the like, and achieves technological operation. Simple, easy industrial production, high-purity effect

Inactive Publication Date: 2014-12-24
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] By searching the literature, the literature on the preparation of picetanol from plants is less disclosed at present, such as the method disclosed in the patent "composition containing picetanol and the preparation method of the composition containing picetanol" is to extract and dry the organic reagent. Therefore, the purity of picatanol is low, the extraction efficiency is low, and the pollution is serious

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take fresh passionflower seeds and crush them, take 10kg, add to 40L saturated lime aqueous solution, heat up to 40°C, keep warm for 1 hour, filter the extract, repeat the extraction 3 times, adjust the combined extract to pH8 with hydrochloric acid, filter and add interception Ultrafiltration in a hollow cellulose ultrafiltration membrane with a molecular weight of 3000, collect the permeate, and then concentrate the permeate through a nanofiltration membrane with a molecular weight cut-off of 200, collect the concentrated solution, adjust the pH to 4 with hydrochloric acid, and heat to 50°C. Add a liquid amount of 0.1% cellulase, keep it warm for 3 hours, concentrate the enzymatic solution, filter after precipitation, and dissolve the crude extract with 95% ethanol under reflux saturation, leave it at room temperature, add a neutral alumina column to absorb impurities, and collect Add an equal volume of water to the column liquid, let the crystals stand, filter the cry...

Embodiment 2

[0019] Take fresh passionflower seeds and crush them, take 10kg, add to 60L saturated lime aqueous solution, heat to 50°C, keep warm for 2 hours, filter the extract, repeat the extraction for 3 times, adjust the combined extract to pH9 with hydrochloric acid, filter and add interception Ultrafiltration in a hollow cellulose ultrafiltration membrane with a molecular weight of 1000, collect the permeate, and then concentrate the permeate through a nanofiltration membrane with a molecular weight cut-off of 100, collect the concentrated solution, adjust the pH to 3 with hydrochloric acid, and heat to 55°C. Add a liquid amount of 0.3% amylase, keep warm for 2 hours for enzymolysis, concentrate the enzymolysis solution, filter after precipitation, reflux and saturate the crude extract with 90% ethanol, leave it at room temperature, add a neutral alumina column to absorb impurities, and collect the next column liquid, add an equal volume of water, leave to crystallize, filter the crys...

Embodiment 3

[0021] Take fresh passionflower seeds and crush them, take 10kg, add to 50L saturated lime aqueous solution, heat to 60°C, keep warm for 1 hour, filter the extract, repeat the extraction 3 times, adjust the combined extract to pH 10 with hydrochloric acid, filter and add interception Ultrafiltration in a hollow cellulose ultrafiltration membrane with a molecular weight of 2000, collect the permeate, and then concentrate the permeate through a nanofiltration membrane with a molecular weight cut-off of 200, collect the concentrated solution, adjust the pH to 5.5 with hydrochloric acid, and heat to 60°C. Add a liquid amount of 0.2% amylase, keep it warm for 4 hours, concentrate the enzymolysis solution, filter after precipitation, reflux and saturate the crude extract with 99% ethanol, leave it at room temperature, add a neutral alumina column to absorb impurities, and collect the next column liquid, add an equal volume of water, leave to crystallize, filter the crystals, dry at l...

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PUM

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Abstract

The invention discloses a method for preparing piceatannol from fresh passion fruit seeds. The method comprises the following steps: 1) taking the fresh passion fruit seeds, crushing, adding a saturated lime solution for soaking and extraction, regulating the pH of an extract solution to 8-10, performing interception and separation with an ultra-filtration membrane, collecting permeate, concentrating the permeate with a nano-filtration membrane with the interception molecular weight of 100-200, regulating the pH of concentrate to 3-5.5, adding a biological enzyme, performing heat preservation and enzymolysis, and performing decompression concentration and precipitation to obtain a crude extract; and 2) dissolving the crude extract with ethanol, adding an aluminum oxide column to adsorb impurities, thus obtaining a solution below the column, adding water, standing, crystallizing and drying to obtain the product. By adopting the method to produce piceatannol, the process is simple and easy to operate, the energy consumption is low and industrial production is easily realized by the method.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for preparing piceatanol from fresh passion fruit seeds. Background technique [0002] Picetanol, CAS registration number 10083-24-6, molecular formula C14H12O4, molecular weight 244.24, molecular structure: [0003] [0004] Picetanol has a strong antioxidant effect, inhibiting non-receptor activating enzymes, spleen tyrosine kinase and lymphocyte-specific protein tyrosine kinase. Passiflora belongs to the Passiflora family. The fruit is rich in 17 kinds of amino acids, multivitamins, carotenoids and various trace elements needed by the human body. The soluble solids are 15-16%, and the total acid content is 3.8-4%. Moderately sweet and sour. Regular consumption of egg fruit and its processed products can help the human body digest, reduce phlegm, treat kidney deficiency, refresh the mind, activate blood and strengthen the body, calm and relieve pain, relieve pressure...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C39/21C07C37/68
CPCC07C37/004
Inventor 苏刘花杨存
Owner NANJING ZELANG AGRI DEV
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