Method for extracting piceatannol from fresh passion fruit seeds
A technology of piceatannol and passionflower, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry and other directions, can solve the problems of pollution, low purity of piceatannol, low extraction efficiency and the like, and achieves technological operation. Simple, easy industrial production, high-purity effect
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Embodiment 1
[0017] Take fresh passionflower seeds and crush them, take 10kg, add to 40L saturated lime aqueous solution, heat up to 40°C, keep warm for 1 hour, filter the extract, repeat the extraction 3 times, adjust the combined extract to pH8 with hydrochloric acid, filter and add interception Ultrafiltration in a hollow cellulose ultrafiltration membrane with a molecular weight of 3000, collect the permeate, and then concentrate the permeate through a nanofiltration membrane with a molecular weight cut-off of 200, collect the concentrated solution, adjust the pH to 4 with hydrochloric acid, and heat to 50°C. Add a liquid amount of 0.1% cellulase, keep it warm for 3 hours, concentrate the enzymatic solution, filter after precipitation, and dissolve the crude extract with 95% ethanol under reflux saturation, leave it at room temperature, add a neutral alumina column to absorb impurities, and collect Add an equal volume of water to the column liquid, let the crystals stand, filter the cry...
Embodiment 2
[0019] Take fresh passionflower seeds and crush them, take 10kg, add to 60L saturated lime aqueous solution, heat to 50°C, keep warm for 2 hours, filter the extract, repeat the extraction for 3 times, adjust the combined extract to pH9 with hydrochloric acid, filter and add interception Ultrafiltration in a hollow cellulose ultrafiltration membrane with a molecular weight of 1000, collect the permeate, and then concentrate the permeate through a nanofiltration membrane with a molecular weight cut-off of 100, collect the concentrated solution, adjust the pH to 3 with hydrochloric acid, and heat to 55°C. Add a liquid amount of 0.3% amylase, keep warm for 2 hours for enzymolysis, concentrate the enzymolysis solution, filter after precipitation, reflux and saturate the crude extract with 90% ethanol, leave it at room temperature, add a neutral alumina column to absorb impurities, and collect the next column liquid, add an equal volume of water, leave to crystallize, filter the crys...
Embodiment 3
[0021] Take fresh passionflower seeds and crush them, take 10kg, add to 50L saturated lime aqueous solution, heat to 60°C, keep warm for 1 hour, filter the extract, repeat the extraction 3 times, adjust the combined extract to pH 10 with hydrochloric acid, filter and add interception Ultrafiltration in a hollow cellulose ultrafiltration membrane with a molecular weight of 2000, collect the permeate, and then concentrate the permeate through a nanofiltration membrane with a molecular weight cut-off of 200, collect the concentrated solution, adjust the pH to 5.5 with hydrochloric acid, and heat to 60°C. Add a liquid amount of 0.2% amylase, keep it warm for 4 hours, concentrate the enzymolysis solution, filter after precipitation, reflux and saturate the crude extract with 99% ethanol, leave it at room temperature, add a neutral alumina column to absorb impurities, and collect the next column liquid, add an equal volume of water, leave to crystallize, filter the crystals, dry at l...
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