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SNP Molecular Marker of Maize Low Phosphorus Response Gene zmarf31 and Its Application

A molecular marker and corn technology, applied in the field of molecular genetics, can solve the time-consuming and labor-intensive problems of QTL positioning, and achieve the effect of improving low phosphorus tolerance and high application value

Inactive Publication Date: 2016-04-06
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, QTL mapping is time-consuming and labor-intensive, and most of the QTL intervals are wide. At present, there are no reports on the development and utilization of molecular markers for the main effect loci of low phosphorus tolerance in maize germplasm improvement and molecular assisted breeding.

Method used

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  • SNP Molecular Marker of Maize Low Phosphorus Response Gene zmarf31 and Its Application
  • SNP Molecular Marker of Maize Low Phosphorus Response Gene zmarf31 and Its Application
  • SNP Molecular Marker of Maize Low Phosphorus Response Gene zmarf31 and Its Application

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1 The acquisition of a SNP molecular marker that is significantly correlated with the total dry weight of the corn low phosphorus response gene ZmARF31 and the determination of its detection primers

[0032] A SNP molecular marker of the corn low phosphorus response gene ZmARF31 significantly correlated with the total dry weight of the present invention is obtained by the following method:

[0033] 1) A total of 331 maize inbred lines from China, the United States and Mexico were collected, and the related populations used for mapping were constructed. The population has rich genetic diversity, including 131 temperate maize inbred lines and 200 tropical / subtropical maize inbred lines.

[0034] 2) Identification of root phenotypes at the seedling stage of related populations. In 2010, Sichuan Agricultural University Duoying Farm Base and in 2012, Sichuan Agricultural University Ya'an Teaching and Experimental Greenhouse, 331 maize inbred lines were obtained in p...

Embodiment 2

[0041] Application test on the total dry weight of SNP marker corn of embodiment 2 of the present invention

[0042] 1) HRM detection of SNP markers in associated populations. The specific method is as follows: randomly select 16 maize inbred lines in the related population, use genomic DNA as a template, and use specific primers (SEQ ID NO. 2 and 3) of the flanking sequence of the site as primers. The reaction system for PCR amplification is: 2×Eva-GreenSuperMix 10 μl, both upstream and downstream primers are 0.25 pmol / μl, DNA template is 100 ng, and double distilled water is added to 20 μl. The amplification reaction was carried out on the CFX96TM real-timePCR (Bio-Rad, USA) detection system, the program was: 98°C pre-denaturation for 90s; 95°C denaturation for 5s, 57°C annealing for 5s, a total of 50 cycles; 70°C to 90°C for each cycle Rise by 0.5°C, read the plate for 1s to draw the melting curve. HRM test results ( figure 1 ) showed that the site-specific primers succe...

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Abstract

The invention provides an SNP molecular marker of a maize low-phosphorus response gene ZmARF31. The SNP marker is positioned at the 148636105bp position of a tenth chromosome, allelic genes are T and G, a site flanking sequence is shown by SEQ ID NO.1, and the SNP marker is obtained by amplifying primers having nucleotide sequences as shown in SEQ ID NO.2-3. The SNP molecular marker is significantly relative to the total dry weight of maize under normal phosphorus level and low-phosphorus stress, the total dry weight of a maize inbred line of which the site gene type is G / G under the normal phosphorus level and low-phosphorus stress is higher than that of an inbred line of which the site gene type is T / T. The molecular marker provided by the invention can be used for assistant breeding of the maize, and can accelerate the maize phosphorus efficient specific material creation and new variety breeding processes.

Description

technical field [0001] The present invention relates to the field of molecular genetics, in particular to molecular markers related to corn low-phosphorus tolerance traits, specifically, to a SNP marker significantly associated with total dry weight in the ZmARF31 gene on chromosome 10 of corn, and the method of amplifying it Primer pairs and the use of the molecular marker. Background technique [0002] Corn is a multivariate crop that integrates grain, economics, and feed. It is also an important industrial raw material and energy crop, and is widely distributed around the world. At the end of the 20th century, with the rapid growth of the global demand for corn, its planting area gradually surpassed that of rice and wheat, and became the food crop with the largest total output in the world. Maize is expected to account for 50 percent of the increase in food production by 2050. Phosphorus is one of the three major nutritional elements necessary for plant growth and devel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 卢艳丽吴锋锴刘玲李嘉徐洁兰海高世斌吴元奇周树峰刘亚西曹墨菊
Owner SICHUAN AGRI UNIV