Electrochemical cell based on in-situ EC-SERS (electrochemical-surface enhanced raman scattering) spectrum chip and detection method of electrochemical cell

Abstract

The invention provides an in-situ EC-SERS (electrochemical-surface enhanced raman scattering) spectrum electrochemical cell. The electrochemical cell comprises an EC-SERS spectrum chip and a sample cell, wherein a working electrode, a counter electrode and a reference electrode are integrated on a substrate of the chip to form the work area of the chip; a nano-structure is arranged on the surface of the working electrode through the electro-deposition reaction; the sample cell is arranged above the chip; a body of the sample cell is contacted with the work area of the chip, the area of the bottom of the body of the sample cell is equal to or larger than the that of the work area of the chip, and a material with the corresponding thickness can be selected according to the specific detection requirements, so that a to-be-detected sample liquid in the sample cell is completely contacted with the three electrodes of the work area; and an insulating layer is arranged on the chip and separates the work area and a wire area. On the premise that the SERS performance is guaranteed, the problem of high probability of liquid leakage is avoided, the electrochemical cell is disposable, the tedious cleaning process is avoided, the detection method is simple and easy, and the detection cost is reduced.

Description

technical field [0001] The patent of the present invention relates to the field of Raman spectroscopy detection, in particular to an in-situ electrochemical-Surface enhanced Raman spectroscopy (Electrochemical-Surface enhanced raman scattering, EC-SERS) micro-electrochemical cell and its detection method. Background technique [0002] In situ EC-SERS is a combined analytical testing method established by combining laser Raman spectrometer with electrochemical testing system. The electrochemical workstation can provide information on electron transfer of molecules on the electrode interface and solution diffusion and migration, while laser Raman spectroscopy can provide information on group vibrations of molecules. Therefore, combining the two can achieve a variety of applications and Research, such as obtaining the redox information of molecules on the electrochemical solid-liquid interface, so as to explore the electrochemical reaction mechanism; pre-enrichment of charged s...

AI Technical Summary

Problems solved by technology

If the structural shape of the commercially available rod-shaped electrode is not changed, there will be problems such as poor sealing at the contact between the smooth electrode rod surface and the liquid pool, and liquid leakage will easily occur. However, Raman signal acquisition usually requires multiple integration and superposition modes. For a certain period of time, once the liquid leaks, it will affect the measurement results, resulting in unsatisfactory results such as reproducibility

(2) The reference electrode is placed at one end of the electrochemical cell. Due to its own weight, it is difficult to keep the liquid cell level as a whole, which affects the collection efficiency of scattered light.

(3) Due to the large number of components in the electrochemical detection system, when changing different solutions to be tested, it is necessary to disassemble the electrodes and sealing rings to complete the cleaning, and the operation is relatively cumbersome

(4) The current electrochemical liquid pool is generally large in volume and requires more solutions, which is not conducive to the testing of precious liquids and biological samples

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