Non-enzyme electrochemical transducer for detecting glucose

An electrochemical and sensor technology, applied in the field of electrochemical sensor preparation, can solve the problems of affecting sensor selectivity, and it is not easy for glucose oxidase to be fixed on electrodes for a long time, and achieve fast electron transfer rate, good analytical performance, and high sensitivity. Effect

Active Publication Date: 2014-12-24
广州衡创测试技术服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the oxidation of hydrogen peroxide needs to be carried out at a higher potential, and many electroactive substances under high potential conditions will be oxidized and interfered, affecting the selectivity of the sensor.
The sensitivity and selectivity o

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0016] Example 1

[0017] An electrochemical sensor for detecting glucose without enzyme, the preparation method is as follows:

[0018] (1) Chloroauric acid (HAuCl 4 ) Preparation of the aqueous solution: Dissolve 1g of gold chloride in 160mL of deionized water, and stir at 40°C for 27 minutes at a stirring rate of 425r / min to obtain gold chloride (HAuCl 4 ) Aqueous solution;

[0019] (2) Preparation of graphene (Gr) dispersion: Disperse 0.1 g of graphene into 80 mL of deionized water, disperse ultrasonically for 20 minutes, and compare it to the 2 mL of chloroauric acid (HAuCl) described in step (1). 4 ) The aqueous solution is mixed and stirred;

[0020] (3) Preparation of graphene / nano-gold (Gr-Au): combine graphene (Gr) with chloroauric acid (HAuCl) 4 ) 50mL of the mixed solution was heated in an oil bath to maintain a constant temperature of 93°C. Under stirring at a rate of 2300r / min, quickly add 45mL of 1% trisodium citrate solution and continue stirring for 60 minutes to obtai...

Example Embodiment

[0023] Example 2

[0024] An electrochemical sensor for detecting glucose without enzyme, the preparation method is as follows:

[0025] (1) Chloroauric acid (HAuCl 4 ) Preparation of the aqueous solution: Dissolve 1g of gold chloride in 100 mL of deionized water, and stir at 30°C for 40 minutes at a stirring rate of 300r / min to obtain gold chloride (HAuCl 4 ) Aqueous solution;

[0026] (2) Preparation of graphene (Gr) dispersion: Disperse 0.1 g of graphene into 50 mL of deionized water, disperse ultrasonically for 20 minutes, and compare it to 0.5 mL of chloroauric acid (HAuCl) described in step (1). 4 ) The aqueous solution is mixed and stirred;

[0027] (3) Preparation of graphene / nano-gold (Gr-Au): combine graphene (Gr) with chloroauric acid (HAuCl) 4 ) 50mL of the mixed solution was heated in an oil bath to maintain a constant temperature of 92°C. Under stirring at a rate of 1500r / min, quickly add 25mL of 1% trisodium citrate solution, and continue to stir for 50min to obtain a pu...

Example Embodiment

[0030] Example 3

[0031] An electrochemical sensor for detecting glucose without enzyme, the preparation method is as follows:

[0032] (1) Chloroauric acid (HAuCl 4 ) Preparation of aqueous solution: Dissolve 1g of gold chloride in 200 mL of deionized water, and stir for 15 minutes at 50°C at a stirring rate of 450r / min to obtain gold chloride (HAuCl 4 ) Aqueous solution;

[0033] (2) Preparation of graphene (Gr) dispersion: Disperse 0.1 g of graphene into 100 mL of deionized water, disperse ultrasonically for 20 minutes, and compare with 5 mL of chloroauric acid (HAuCl) described in step (1). 4 ) The aqueous solution is mixed and stirred;

[0034] (3) Preparation of graphene / nano-gold (Gr-Au): combine graphene (Gr) with chloroauric acid (HAuCl) 4 ) 50mL of the mixed solution was heated in an oil bath to maintain a constant temperature of 98°C. Under stirring at a rate of 3000r / min, quickly add 50mL of 1% trisodium citrate solution, and continue stirring for 90 minutes to obtain a pu...

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PUM

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Abstract

The invention relates to a non-enzyme electrochemical transducer for detecting the glucose content on the basis of graphene/nanogold modified electrode (Gr-Au/GC). According to the non-enzyme electrochemical transducer for detecting the glucose, chloroauric acid (HAuCl4) and graphene (Gr) are used for preparing a modified electrode, the prepared modified electrode is extremely highly active on oxidation of the glucose, highly dispersed nanoparticles in the modified electrode provide large superficial area for electro-catalysis reaction, the detection sensitivity is improved, the electron transfer rate is increased, and analysis performance of the glucose is improved. According to the non-enzyme electrochemical transducer for detecting the glucose, the detection sensitivity of the glucose is high, the detection limit is low, and the detection range is wide.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical sensor, in particular to a preparation method of an enzyme-free electrochemical sensor for glucose detection. Background technique [0002] Sensors for glucose detection have become one of the most popular biosensors due to their importance in clinical diagnosis, pharmaceutical analysis, and food industry. [0003] The most widely used glucose sensor based on enzymatic reaction, its most basic principle is: using the immobilized glucose oxidase membrane as a recognition device to convert the detected amount of glucose into a usable output signal. The glucose sensor basically consists of an enzyme membrane and a Clark oxygen electrode or a hydrogen peroxide electrode. Under the catalysis of glucose oxidase, the oxidation reaction of glucose consumes oxygen and produces gluconolactone and hydrogen peroxide. Glucose oxidase is immobilized by the semipermeable membrane on the surface clo...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30G01N27/38
Inventor 孙京华潘正海
Owner 广州衡创测试技术服务有限公司
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