A method for rapid detection of tyrosine concentration

A tyrosine and concentration technology, which is applied to measurement devices, instruments, electrochemical variables of materials, etc., can solve the problems of insensitive detection of mixed samples, inability to meet rapid detection, complicated and time-consuming pretreatment, and achieve good surface and interface characteristics. and electrochemical, eliminate electronic interference factors, the effect of fast electron transfer rate

Active Publication Date: 2019-05-17
铜陵华兴精细化工有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The methods for determining tyrosine mainly include amino acid automatic analyzer, spectrophotometry, high-performance liquid chromatography, mass spectrometry, nuclear magnetic resonance spectroscopy and capillary electrophoresis, etc. Although these techniques are widely used, the instruments used are expensive and advanced. Complex and time-consuming processing, high reagent consumption, long analysis time, insensitive defect detection of mixed samples, high detection limit, cannot meet the needs of on-site rapid detection

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  • A method for rapid detection of tyrosine concentration
  • A method for rapid detection of tyrosine concentration

Examples

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Embodiment 1

[0028] A method for rapidly detecting tyrosine concentration, comprising the following steps:

[0029] Step 1. Prepare multiple PBS buffer solutions containing different concentrations of tyrosine. The pH of the PBS buffer solution is 7.4, the concentration is 0.1mol / L, and the tyrosine concentration is 5.0×10 -6 mol / L, 1×10 -5 mol / L, 2.5×10 - 5 mol / L, 4.5×10 -5 mol / L, 7×10 -5 mol / L, 1×10 -4 mol / L, 1.5×10 -4 mol / L, 2×10 -4 mol / L, 3×10 -4 mol / L. Use the glassy carbon electrode as the working electrode to build a three-electrode system, set the initial potential to 0.4V, the end potential to 1.0V, and then set the following pulse parameters: the potential increment is 0.004V, the square wave frequency is 50Hz, and the square wave amplitude is 0.05 V, the waiting time is 2s. Using differential pulse voltammetry, measure and record the differential pulse voltammetry curves of multiple PBS buffer solutions containing different concentrations of tyrosine, and record the pe...

Embodiment 2

[0035] A method for rapid detection of tyrosine concentration, the assay steps are the same as in Example 1, wherein,

[0036] The glassy carbon electrode has been polished, specifically as follows: soak the glassy carbon electrode in an aqueous solution of purple sweet potato powder for 20 minutes, take out the glassy carbon electrode and soak it in a carbon tetrachloride solution of soybean lecithin for 20 minutes, and then use 95% of the mass fraction in turn. Ultrasonic cleaning with ethanol aqueous solution and double distilled water for 5 minutes respectively, and then grinding with alumina powder with a particle size of 0.3 μm and 0.05 μm in sequence, then ultrasonic cleaning with 95% ethanol aqueous solution and double distilled water for 5 minutes, and finally using nitrogen gas Just blow dry;

[0037] Wherein, the preparation of the aqueous solution of purple sweet potato powder is as follows: purple sweet potato powder and deionized water are mixed in a mass volume ...

Embodiment 3

[0045] A method for rapid detection of tyrosine concentration, the assay steps are the same as in Example 1, wherein,

[0046] The glassy carbon electrode is polished, specifically: soak the glassy carbon electrode in an aqueous solution of purple sweet potato powder for 30 minutes, take out the glassy carbon electrode and soak it in a carbon tetrachloride solution of soybean lecithin for 30 minutes, and then use 95% of the mass fraction in turn. Ultrasonic cleaning with ethanol aqueous solution and double distilled water for 10 minutes respectively, and then grinding with alumina powder with a particle size of 0.3 μm and 0.05 μm in sequence, then ultrasonic cleaning with 95% ethanol aqueous solution and double distilled water for 10 minutes, and finally using nitrogen gas Just blow dry;

[0047] Wherein, the preparation of the aqueous solution of purple sweet potato powder is as follows: purple sweet potato powder and deionized water are mixed at a mass volume ratio of 1g:20m...

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Abstract

The invention discloses a method for quickly detecting concentration of tyrosine. The method comprises the following steps of (1) building a three-electrode system employing a glassy carbon electrode as a working electrode, measuring and recording differential pulse voltage-current curves of a plurality of PBS buffer solutions containing tyrosine of different concentration by using a differential pulse voltammetry separately and recording couponing voltage strength peaks; (2) drawing a standard curve and calculating a linear equation by employing the difference value between the voltage strength peak of each PBS buffer solution containing the tyrosine and the voltage strength peak of the PBS buffer solution free of the tyrosine as a vertical coordinate and the concentration of each part of tyrosine as a transverse coordinate; and (3) obtaining the voltage strength peak of a to-be-detected solution obtained in the step (1) and substituting the voltage strength peak into the liner equation to obtain the concentration of the tyrosine of the to-be-detected solution. The method has the advantages of being simple in operation, fast in detection and high in sensitivity, and high-sensitivity identification of the tyrosine in a mixed sample solution can be carried out.

Description

technical field [0001] The invention relates to the field of tyrosine concentration detection. More specifically, the present invention relates to a method for rapidly detecting the concentration of tyrosine. Background technique [0002] Tyrosine (Tyr) is an aromatic oxyacid, which can be synthesized from phenylalanine in the body, and is an important semi-essential amino acid for the human body. The metabolic disorder of tyrosine can lead to the occurrence of various genetic diseases, such as tyrosinemia, akaponic acid disease, etc., and has a certain correlation with liver and kidney diseases, nervous system degenerative diseases, malignant tumors, etc. . Tyrosine is an indispensable amino acid for the human body. It is the precursor for the synthesis of neurotransmitter catecholamines. Without tyrosine, the human body will have abnormal growth and mental retardation. Therefore, tyrosine in food has generally begun to be paid attention to at home and abroad. Addition o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48G01N27/30G01N27/36G01N27/38
CPCG01N27/308G01N27/36G01N27/38G01N27/48
Inventor 肖琦黄珊冯萌萌吴子华
Owner 铜陵华兴精细化工有限公司
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