Composite preparation capable of promoting restoration of skin wound due to diabetes and preparation method thereof

A compound preparation and skin wound technology, applied in the field of medicine, can solve the problems of increasing the risk of infection, inhibiting inflammatory response, and having little effect on skin wound treatment, and achieving the effect of promoting rapid healing and inhibiting inflammatory response.

Inactive Publication Date: 2014-12-31
SOUTHERN MEDICAL UNIVERSITY
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although traditional skin wound repair materials have a good therapeutic effect on ordinary skin wounds, they have little effect on the treatment of severe skin wounds such as diabetic skin ulcers. Restorative materials are not able to suppress the inflammatory response well
On the other hand, autologous keratinocytes and f

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composite preparation capable of promoting restoration of skin wound due to diabetes and preparation method thereof
  • Composite preparation capable of promoting restoration of skin wound due to diabetes and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0021] Example 1

[0022] Isolation and culture of BMSCs cells:

[0023] Choose healthy male C57BL / 6 mice (purchased from the Animal Experiment Center of Southern Medical University) at the age of 8 weeks. After dislocation and sacrifice, they are soaked in 75% ethanol for 5 minutes; the femurs and tibias are taken out under aseptic conditions and washed with sterile PBS buffer; Cut both ends of the bone, expose the bone marrow cavity, use a 1ml syringe to draw the culture medium (DMEM containing 10v / v% fetal calf serum, 1 v / v% double antibody, 1 v / v% glutamine) into the bone marrow cavity and flush out Wash the bone marrow repeatedly 3 to 5 times until the bone marrow cavity turns white; transfer the culture medium containing bone marrow cells into a centrifuge tube, centrifuge at 1000 rpm for 5 minutes, discard the supernatant, and resuspend in L-DMEM medium containing 10% fetal bovine serum Cells are transferred into a petri dish and placed in 37℃, 5% CO 2 Incubate in an incu...

Example Embodiment

[0024] Example 2

[0025] Preparation of thermosensitive hydrogel-BMSCs compound biological preparation 1#:

[0026] After the third generation of BMSCs grow to 80%, change to serum-free DMEM medium, 37℃, 5% CO 2 After culturing in an incubator for 6 hours, digest with 0.25% trypsin, centrifuge, discard the supernatant, resuspend in DHanks solution and count, centrifuge to collect the cell pellet, add temperature-sensitive hydrogel according to the cell count results, and mix well. The final concentration of prepared cells is 1×10 5 A / ml compound biological preparation for use.

Example Embodiment

[0027] Example 3

[0028] Preparation of temperature-sensitive hydrogel-BMSCs composite biological preparation 2#:

[0029] After the 6th generation BMSCs grow to 60%, change to serum-free DMEM medium, 37℃, 5% CO 2 After culturing in an incubator for 12 hours, digest with 0.25% trypsin, centrifuge, discard the supernatant, resuspend in DHanks solution and count, centrifuge to collect the cell pellet, add temperature-sensitive hydrogel according to the cell count result, and mix well. The final concentration of prepared cells is 1×10 7 A / ml compound biological preparation for use.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a composite preparation capable of promoting restoration of skin wound due to diabetes and a preparation method thereof. The method successfully prepare a temperature sensitive hydrogel-BMSCs composite biological preparation, the preparation is used for restoration of skin wound due to diabetes mice, and the result displays that the composite preparation can inhibit the skin wound inflammation due to diabetes mice and can promote rapid heal of wound. The composite preparation provides important drug reference for treating skin wound of clinical diabetes and other chronic skin ulcer.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a compound preparation for promoting diabetic skin wound repair and a preparation method thereof. Background technique [0002] Diabetes mellitus is a chronic metabolic disease characterized by persistent hyperglycemia. The greatest threat of diabetes to patients comes from its complications, such as retinopathy, diabetic nephropathy, neuropathy, and refractory wounds. Among them, the repair of diabetic refractory wounds and the treatment of spontaneous skin ulcers not only plague clinicians, but also are major problems faced by the medical field. Complications of diabetes have become the main cause of disability and death of diabetic patients, and also the main factor of increasing medical expenses. In recent years, many new theories and viewpoints have been put forward for the mechanism of diabetic skin wound healing, mainly focusing on the following aspects: 1. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K35/28A61K47/32A61K9/06A61P17/02A61P3/10
Inventor 张琳张璐陈世萱
Owner SOUTHERN MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap