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Method of degrading phosphate tri(2-chloroethyl) ester by adopting bacillus thuringiensis

A technology of Bacillus thuringiensis and Bacillus aureus, applied in the field of microbial degradation of organic pollutants, can solve the problem of low efficiency of organophosphorus flame retardants

Active Publication Date: 2015-01-07
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in practical applications, the efficiency of aerobic microorganisms to degrade organophosphorus flame retardants is often not high, so the screening and application of aerobic degradation bacteria for organophosphorus flame retardants still has certain research significance

Method used

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  • Method of degrading phosphate tri(2-chloroethyl) ester by adopting bacillus thuringiensis
  • Method of degrading phosphate tri(2-chloroethyl) ester by adopting bacillus thuringiensis
  • Method of degrading phosphate tri(2-chloroethyl) ester by adopting bacillus thuringiensis

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Embodiment 1

[0023] 1. Isolation of strains

[0024] (1) Collect the activated sludge from the sewage treatment plant and place it in a glass acclimation device with a length of 31.2cm, a width of 22.0cm and a height of 22.5cm, and use an air compressor without adding nutrients or exchanging water in and out. Intermittent aeration for 3 days. Then change the water, discharge about 10L of mud-water mixture in the device, add nutrient solution and carry out uninterrupted aeration. After cultivating for 10 days, start to add tris (2-chloroethyl) phosphate-acetone solution when adding nutrient solution, keep the concentration of tris (2-chloroethyl) phosphate in the device at about 1ppm, and according to the SBR process ( Water intake, aeration, standing, draining and idle) were carried out for domestication and cultivation, and the water was changed every other day for one month of cultivation and domestication. Preparation of culture medium: 0.6g / L glucose, 0.8g / L anhydrous sodium acetate,...

Embodiment 2

[0034] Example 2 Degradation Analysis of Tris(2-Chloroethyl) Phosphate by Bacillus thuringiensis

[0035] Under sterile conditions, Bacillus thuringiensis was inoculated into a 250mL Erlenmeyer flask containing 100mL of sterilized enrichment medium, placed in a constant temperature shaker, 30°C, 120rpm, and cultivated for 48h. The main components of enrichment medium EM (Enrichment Medium) are: 10g / L fish meal peptone, 5g / L yeast powder, 5g / L NaCl, 1000mL distilled water, pH=6.8~7.2.

[0036] Centrifuge the enriched cultured bacteria in a 10mL sterilized centrifuge tube at a speed of 6000r / min for 10min, collect the bacteria, wash repeatedly with sterile normal saline and centrifuge for 2-3 times, and finally resuspend with an equal volume of normal saline Be made into the bacterium suspension of certain concentration, inoculate in the tris (2-chloroethyl) phosphate degradation medium by the amount of 10% and carry out degradation experiment, the sample is measured by GC-MS, a...

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Abstract

The invention relates to a method of degrading phosphate tri(2-chloroethyl) ester by adopting bacillus thuringiensis. The method comprises the following steps: at 30 DEG C, inoculating bacillus thuringiensis into a phosphate tri(2-chloroethyl) ester degrading culture medium; after constant-temperature shaking table degradation culture, measuring the concentration of residual phosphate tri(2-chloroethyl) ester in culture fluid by adopting gas chromatography-mass spectrometry (GC-MS), and then analyzing the degrading effect of the bacterial strain on phosphate tri(2-chloroethyl) ester. The method has high environmental adaptation, relatively high degradation degree on organic phosphorus fire retardants, and relatively low cost in comparison with the cost of other methods.

Description

technical field [0001] The invention belongs to the field of microbial degradation of organic pollutants, in particular to a method for degrading tris(2-chloroethyl) phosphate by bacillus thuringiensis. Background technique [0002] Organophosphorus flame retardants have good flame retardant properties, good compatibility with polymer substrates, and have the characteristics of water resistance, heat resistance, and migration resistance. Fuel. At present, during the production, use and waste recycling of products containing organophosphorus flame retardants, they are very easy to escape and volatilize from these products and enter the surrounding environment, and then diffuse and distribute in various environmental media. At present, organophosphorus flame retardants are common pollutants in sewage. It is generally believed that the effluent of sewage treatment plants is the main source of organophosphorus flame retardants in surface water; The pollution of flame retardant...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F101/36
CPCC02F3/34C02F2101/36
Inventor 赵晓祥赵银平孙璐璐耿艳芳
Owner DONGHUA UNIV
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