Bioreactor based on enzymatic-photocatalytic synergistic effect as well as preparation method and application of bioreactor in degradation of organic dye

A bioreactor, synergistic effect technology, applied in the application field of degrading organic dyes, can solve the problems of limited catalytic efficiency and narrow response range, and achieve the effect of improving catalytic efficiency, strong environmental adaptability and improving adaptability

Inactive Publication Date: 2015-01-07
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

TiO 2 It is the most successful semiconductor photocatalyst at present. It not only has good chemical stability and outstanding catalytic effect, but also has excellent biocompatibility. The disadvantage is that the catalytic efficiency is limited due to the narrow response range to light.

Method used

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  • Bioreactor based on enzymatic-photocatalytic synergistic effect as well as preparation method and application of bioreactor in degradation of organic dye
  • Bioreactor based on enzymatic-photocatalytic synergistic effect as well as preparation method and application of bioreactor in degradation of organic dye

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preparation example Construction

[0025] The organic dye degradation bioreactor of the present invention is based on the enzymatic-photocatalytic synergistic effect of chloroperoxidase-loaded TiO 2 Thin film, its preparation method and the method for organic dye degradation comprise the following steps:

[0026] (1) Analytical pure butyl titanate Ti(OC 4 h 9 ) 4 with ethanol C 2 h 5 OH is fully mixed according to the volume ratio of 1: (1~5), slowly add 1mol / L dilute HCl dropwise, adjust the pH value of the mixed solution to 2.0~8.0, stir magnetically at room temperature for 1~4h, and obtain a light yellow transparent sol A. Sol A was aged at room temperature for 12-24 hours to obtain gel B, namely TiO 2 Gel; aging makes the macromolecular structure in gel B more uniform, which is conducive to subsequent film formation;

[0027](2) Take the glass substrate and immerse it in analytically pure anhydrous ethanol and ultrasonically clean it for 10-15 minutes, then immerse it in analytically pure acetone and ...

Embodiment 1

[0037] (1) Analytical pure butyl titanate Ti(OC 4 h 9 ) 4 with ethanol C 2 h 5 OH was fully mixed according to the volume ratio of 1:1, and 1mol / L dilute HCl was slowly added dropwise to adjust the pH value of the mixed solution to 2.0, and magnetically stirred at room temperature for 2 hours to obtain light yellow transparent sol A, which was left to stand at room temperature After 24h, gel B was obtained;

[0038] (2) Take the glass substrate and immerse it in analytically pure absolute ethanol and ultrasonically clean it for 10 minutes, then immerse it in analytically pure acetone and ultrasonically clean it for 15 minutes, finally rinse it with deionized water, and dry it with nitrogen gas for later use;

[0039] (3) Coating by dipping-pulling method, immersing the cleaned glass substrate in gel B and standing for 10s, pulling the coating vertically and uniformly at a pulling speed of 2cm / min, and immediately putting it in an oven at 40°C dried to obtain TiO on a glas...

Embodiment 2

[0044] (1) Analytical pure butyl titanate Ti(OC 4 h 9 ) 4 with ethanol C 2 h 5 OH was fully mixed according to the volume ratio of 1:3, and 1mol / L dilute HCl was slowly added dropwise to adjust the pH value of the mixed solution to 4.0, and magnetically stirred at room temperature for 4 hours to obtain light yellow transparent sol A, which was left to stand at room temperature After 12h, gel B was obtained;

[0045] (2) Take the glass substrate and immerse it in analytically pure absolute ethanol and ultrasonically clean it for 10 minutes, then immerse it in analytically pure acetone and ultrasonically clean it for 15 minutes, finally rinse it with deionized water, and dry it with nitrogen gas for later use;

[0046] (3) Coating by dipping-pulling method, immersing the cleaned glass substrate in gel B and standing for 10s, pulling the coating vertically and uniformly at a pulling speed of 2cm / min, and immediately putting it in an oven at 25°C dried to obtain TiO on a glas...

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Abstract

The invention relates to a bioreactor based on enzymatic-photocatalytic synergistic effect as well as a preparation method and application of the bioreactor in degradation of an organic dye. The bioreactor is a TiO2 film loaded with chloroperoxidase, wherein the content of chloroperoxidase (CPO) is 5-30mg / cm<2>. According to the bioreactor, by selecting a semiconductor photocatalyst TiO2 as a carrier for immobilizing CPO, the effective immobilization of CPO is achieved, the adaptability of CPO to the reaction environment is improved, the loss is avoided and the waste is reduced; and meanwhile, the photocatalytic effect makes up the reduction in catalytic activity caused by the enzyme surface comformational change, the catalyst TiO2 is more suitable for working under sunlight irradiation conditions, the synergistic effect can be generated between the two catalysts and thus the catalytic efficiency is significantly improved and the decolorization rate can be significantly improved when the bioreactor is applied in degradation of organic dyes.

Description

technical field [0001] The invention relates to the field of catalytic degradation of organic dyes, in particular to a bioreactor based on an enzymatic-photocatalytic synergistic effect, a preparation method thereof and an application in degrading organic dyes. Background technique [0002] Printing and dyeing industrial wastewater is highly toxic, polluting, and difficult to treat, which is an urgent problem to be solved in the field of environmental protection. Chloroperoxidase (CPO for short) is a heme peroxidase extracted from marine fungi, which can efficiently catalyze the oxidation and degradation of various dyes. Studies have found that CPO with a concentration of less than 0.4 μM can decompose within 5 minutes. Internal catalytic degradation of typical anthraquinones, triphenylmethanes, azo dyes, and the degradation efficiency of all kinds of dyes are greater than 90%. However, the application of CPO to industrial wastewater treatment faces practical problems such ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/40C02F1/30C02F1/58
CPCY02W10/37C12M21/18C02F3/342C02F2101/308C02F2305/10
Inventor 蒋育澄卢靖胡满成李淑妮翟全国
Owner SHAANXI NORMAL UNIV
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