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Method for extracting levodopa from velvet beans

The technology of levodopa and cat beans is applied in the extraction field in the field of biotechnology, which can solve the problems of easy contamination of membranes, evaporation of large extractants, influence on product quality, etc., so as to increase process time and cost, increase content and extraction efficiency. , the effect of easy operation

Inactive Publication Date: 2015-01-28
桂林三宝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the membrane used is easily polluted, which will affect the product quality; and the membrane has a short service life and needs to be replaced regularly, which increases the cost
The traditional extraction process mostly uses the acid-water-alcohol method, and the yield of levodopa is only about 1.5%. The biggest disadvantage of this process is that a large amount of extractant needs to be evaporated, which requires high energy consumption, low yield, large investment in equipment, and high cost.

Method used

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  • Method for extracting levodopa from velvet beans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for extracting levodopa from cat beans, specifically comprising the steps of:

[0022] 1) Raw material processing: crush the cat beans;

[0023] 2) High-pressure extraction: Put the processed raw materials into a high-pressure tank, add the same weight of pure water, and pressurize to 4kgf / cm 2 , to make the pressure uniform, and keep it for 10 minutes, return to normal pressure, filter, and obtain the extract;

[0024] 3) Separation: Carry out two-phase extraction of cat beans to obtain the upper phase and the lower phase of the extraction. The two-phase system used in the two-phase extraction is composed of polyethylene glycol, sodium hydroxide and water. Polyethylene glycol The mass percent in the two-phase aqueous system is 25%, the mass percent of sodium hydroxide in the two-phase aqueous system is 3%, the balance is water, and the weight average molecular weight of the polyethylene glycol is 1000 -8000;

[0025] 4) Subsequent treatment: The upper phase...

Embodiment 2

[0027] A method for extracting levodopa from cat beans, specifically comprising the steps of:

[0028] 1) Raw material processing: crush the cat beans;

[0029] 2) High-pressure extraction: Put the processed raw materials into a high-pressure tank, add the same weight of pure water, and pressurize to 4kgf / cm 2 , make the pressure uniform, and keep it for 15 minutes, return to normal pressure, filter, and obtain the extract;

[0030] 3) Separation: Carry out two-phase extraction of cat beans to obtain the upper phase and the lower phase of the extraction. The two-phase system used in the two-phase extraction is composed of polyethylene glycol, sodium hydroxide and water. Polyethylene glycol The mass percent in the two-phase aqueous system is 20%, the mass percent of sodium hydroxide in the two-phase aqueous system is 5%, the balance is water, and the weight-average molecular weight of the polyethylene glycol is 1000 -8000;

[0031] 4) Subsequent treatment: The upper phase of...

Embodiment 3

[0033] A method for extracting levodopa from cat beans, specifically comprising the steps of:

[0034] 1) Raw material processing: crush the cat beans;

[0035] 2) High-pressure extraction: Put the processed raw materials into a high-pressure tank, add the same weight of pure water, and pressurize to 4kgf / cm 2 , to make the pressure uniform, and keep it for 12 minutes, return to normal pressure, and filter to obtain the extract;

[0036] 3) Separation: Carry out two-phase extraction of cat beans to obtain the upper phase and the lower phase of the extraction. The two-phase system used in the two-phase extraction is composed of polyethylene glycol, sodium hydroxide and water. Polyethylene glycol The mass percent in the two-phase aqueous system is 30%, the mass percent of sodium hydroxide in the two-phase aqueous system is 1%, the balance is water, and the weight-average molecular weight of the polyethylene glycol is 1000 -8000;

[0037] 4) Subsequent treatment: The upper pha...

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Abstract

The invention discloses a method for extracting levodopa from velvet beans. The method includes the following steps: raw material processing, high-pressure extraction, aqueous two-phase extraction, desalination, standing, devitrification, and drying to obtain the levodopa. The method provided by the invention has the advantages of high levodopa content, high extraction rate, simple process, suitability for production, low production cost and the like.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting levodopa from cat beans. Background technique [0002] Cat beans (Mucuna cochinchine-sis (Lour) Tang et Wang.), aliases: Li beans, dragon claw beans, tiger claw beans, dog claw beans, English name: velvet bean. Fabaceae Li beans are annual herbaceous vines. Stem cylindrical, tendril 2-10m long, much branched. Three compound leaves, leaflets oblong-ovate. Racemes, butterfly corolla, nearly white or purple. Native to southern and eastern Asia. China is mainly distributed in South China, Southwest China and Zhejiang, Jiangxi, Hunan, Guangxi and other provinces. It likes a warm and humid climate, drought-tolerant, barren-resistant, and not waterlogging. Cat bean is a drought-resistant, thin-resistant, and highly adaptable crop. There are many rhizobia in the root, which can improve soil fertility. Cat beans are full of treasures. Bea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C229/36C07C227/40
Inventor 卢照凯周晓青谢冬养钟德品卢桦香盘秋梅
Owner 桂林三宝生物科技有限公司
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