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A kind of decolorization bacterium and application

A technology for bacteria and decolorization, which is applied in the direction of bacteria, process wastewater treatment, water/sludge/sewage treatment, etc. It can solve the problems of difficult application and microbial coordination, and achieves wide application prospects, fast growth, and is not easy to lose and die. Effect

Inactive Publication Date: 2017-04-05
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main microorganisms for biological decolorization are white-rot fungi, algae and photosynthetic bacteria. These microorganisms are often unable to cooperate with ordinary aerobic heterotrophic sewage treatment systems due to their special growth characteristics or physiological structures, which brings difficulties to the application.

Method used

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  • A kind of decolorization bacterium and application
  • A kind of decolorization bacterium and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Screening and Identification of Acinetobacter YH-02 (Acinetobacter sp.).

[0032] The experimental materials come from the silt on the banks of the Yi River in Linyi City, Shandong Province.

[0033] Take 0.1ml of the sludge dilution, spread it on the screening medium plate, cultivate it in a 30°C incubator, pick a single colony and repeat the streak purification 2-3 times to obtain the purified strain. After 16S rDNA identification, it belongs to Acinetobacter sp., the phylogenetic tree is shown in the appendix of the manual figure 1 , having the sequence shown in 1 in the sequence table.

Embodiment 2

[0035] Decolorization experiment 1 of Acinetobacter sp. YH-02.

[0036] The object of treatment is papermaking wastewater, and the operation mode is shaking culture in shake flasks.

[0037] The specific implementation steps are as follows: the strains are inoculated into LB medium, and cultured with shaking at 30°C until the bacterial concentration is O.D. 600 ≈2.0, inoculated in papermaking wastewater with 8% inoculation amount, cultured at 35°C for 5 days, and maintained dissolved oxygen above 3.0mg / L; centrifuged to take supernatant, and measured according to "Determination of Water Quality Chromaticity (GB11903-89)" Chromaticity, measure COD according to "Dichromate Method (GB11914-89)". The results are shown in Table 1 below.

[0038] Table 1

[0039] Chroma (degrees) COD (mg / L) flooded 560 2800 out of water 40 274 Removal rate (%) 92.9 90.2

Embodiment 3

[0041] Decolorization experiment 2 of Acinetobacter sp. YH-02.

[0042] The treatment object is papermaking wastewater, and the operation mode is aeration cultivation in a 3L SBR (sequencing batch bioreactor) system.

[0043] The specific implementation steps are as follows: the strains are inoculated into LB medium, and cultured with shaking at 30°C until the bacterial concentration is O.D. 600 ≈2.0, inoculated in papermaking wastewater with 5% inoculation amount, cultured at 30°C for 3 days, and maintained dissolved oxygen above 3.0mg / L; centrifuged to take supernatant, and measured according to "Determination of Color of Water Quality (GB11903-89)" Chromaticity, measure COD according to "Dichromate Method (GB11914-89)". The results are shown in Table 2 below.

[0044] Table 2

[0045] Chroma (degrees) COD (mg / L)

[0046] flooded 560 2800 out of water 60 397.6 Removal rate (%) 89.3 85.8

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Abstract

The present invention provides a kind of decolorizing bacterium and application, and described decolorizing bacterium is Acinetobacter YH-02 (Acinetobacter sp.), and its preservation number is CGMCC No.9413, utilizes this bacterial strain to realize the decolorization of papermaking wastewater and the removal of COD, The strain was inoculated into LB medium, cultured with shaking at 30°C until the bacterial concentration was O.D600≈2.0, inoculated into papermaking wastewater with an inoculum of 5-8%, cultured at 30-35°C for 3-5 days, and maintained dissolved oxygen Above 3.0mg / L; take the supernatant by centrifugation, measure the chroma and COD, the results show that the decolorization effect is obvious, and it has a certain COD removal ability, and it has advantages compared with physical and chemical decolorization methods and traditional decolorization microorganisms. It has good application value in wastewater treatment engineering.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a decolorizing bacterium and its application. Background technique [0002] The paper industry uses wood, straw, reeds, rags, etc. as raw materials, and cellulose is separated by high-temperature and high-pressure cooking to make pulp. In the production process, the waste part of the final discharged raw material is called papermaking black liquor. Black liquor contains lignin, cellulose, volatile organic acids, etc., which has an odor and is highly polluting. The pollutants contained in it account for more than 90% of the total pollution discharge of the paper industry. The color is deep, the smell is heavy, there are many foams, and a large amount of dissolved oxygen in the water is consumed, which seriously pollutes the water source; the most serious environmental pollution in the middle section of the water is the chlorine-containing wastewater produced in the bleaching process,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F103/28
CPCC02F3/34C02F2103/28C12N1/205C12R2001/01
Inventor 李修岭李强徐树建刘金民
Owner LINYI UNIVERSITY