Unlock instant, AI-driven research and patent intelligence for your innovation.

Ovarian Cancer Cell Detection Kit for Whole Blood

A detection kit and ovarian cancer cell technology, applied in the fields of biotechnology and biomedicine, can solve the problems of low detection sensitivity and low signal, and achieve the effects of high photochemical stability, high efficiency and fast speed

Active Publication Date: 2016-05-11
NANCHANG UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both have their limitations
At present, there are many reports on the preparation of fluorescent immunoprobes by combining QDs with antibodies, but QDs immunofluorescent probes still have problems such as low signal and low detection sensitivity when detecting very small amounts of substances.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ovarian Cancer Cell Detection Kit for Whole Blood
  • Ovarian Cancer Cell Detection Kit for Whole Blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Ovarian cancer cell detection kit for detection of ovarian cancer SKOV3 cells in whole blood

[0033] 1 Preparation of biotinylated BSA-FA

[0034] 1) FA-PEG-NH 2 Coupling: Dissolve 6 mg of FA in 600 μL dimethyl sulfoxide, add 3 mg of DCC, 3 mg of NHSS and 60 μL of triethylamine to react in a dark room for 4 hours, add 30 mg of diaminopolyethylene glycol and react overnight in a dark room; after the reaction is complete, centrifuge and store at -20 °C Precipitate with acetone, wash with ethyl acetate three times, dry in a fume hood and store in the dark; 2) Coupling of FA-PEG-BSA: FA-PEG-NH 2 Dissolve 5mg, EDC2.5mg, NHS2.5mg in 500μL BB with pH 7 and react together at room temperature for 10min; add BSA8.35mg, react at room temperature for 2h, dialyze and purify for 3 times, and store in refrigerator at 4℃; 3) Biotinylated BSA-FA Preparation: mix long-chain biotin: FA-PEG-BSA at a molar ratio of 20:1, incubate at room temperature for 30 min, dialyze the mixe...

Embodiment 2

[0065] Example 2 Application of Ovarian Cancer Cell Detection Kit for Whole Blood in Whole Blood of Ovarian Cancer Patients

[0066] Take the kit of the present invention: including 1) HE4 goat polyclonal antibody; 2) biotinylated BSA-FA; 3) streptavidinized nano magnetic beads SA-IO; 4) streptavidin The tinylated QDs are SA-QDs; 5) biotinylated anti-HE4 rabbit anti-goat IgG;

[0067] Wherein, each material preparation method is as follows:

[0068] 1 Preparation of biotinylated BSA-FA

[0069] 1) FA-PEG-NH 2 Coupling: FA6mg was dissolved in 600μL dimethyl sulfoxide, DCC3mg, NHS3mg and triethylamine 60μL were added to react in a darkroom for 4 hours, and diaminopolyethylene glycol 30mg was added to react overnight in a darkroom; after the reaction was complete, centrifuge and store at -20℃ Precipitate with acetone, wash with ethyl acetate three times, dry in a fume hood and store in the dark; 2) Coupling of FA-PEG-BSA: FA-PEG-NH 2 Dissolve 5mg, EDC2.5mg, NHS2.5mg in 500μL ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention provides a whole blood-oriented ovarian carcinoma cell detection kit. The kit mainly comprises HE4 goat polyclonal antibodies capable of specifically recognizing the antigen of the ovarian carcinoma cell human epididymis protein (HE4), biotinylated bovine serum albumin (BSA)-folic acid (FA), streptavidin-modified nano magnetic beads (SA-IO), streptavidin-modified QDs (SA-QDs), biotinylated anti-HE4 rabbit anti-goat IgG, a connecting reagent and the like. The kit is capable of efficiently separating out the carcinoma cells in the whole blood of an ovarian carcinoma patient by virtue of the enrichment characteristic of the immunomagnetic beads, and realizing multiple-signal synergetic amplification by specially binding with the targeted ovarian carcinoma cells by use of the SA-QDs and a biotinylated HE4 antigen-antibody complex. The kit has high sensitivity and specificity, and has an extremely wide application prospect in early detection of the ovarian carcinoma.

Description

technical field [0001] The invention belongs to the fields of biotechnology and biomedicine. Specifically, the present invention provides a kit for detecting ovarian cancer. Background technique [0002] Ovarian cancer is a common malignant tumor in gynecology. Its incidence rate ranks third among female reproductive system tumors, but its case fatality rate ranks first. Most ovarian cancers are diagnosed at an advanced stage, and their 5-year survival rate is only 30%. Early diagnosis and treatment of ovarian cancer are important factors to improve the prognosis of patients and increase their survival rate. Currently, there are two common clinical screening methods for ovarian cancer: 1) transvaginal ultrasonography; 2) serum CA125 detection. Both have their limitations. Studies have shown that the appearance of circulating tumor cells (CTCs) in the peripheral blood of cancer patients is earlier than that of visible solid tumors. Therefore, finding a method for highly sp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/531
CPCG01N33/57449
Inventor 许恒毅傅芬聂丽菊叶称连张婉怡李福来
Owner NANCHANG UNIV