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Method of intuitively screening microorganism strains for inhibiting aflatoxin

A technology of aflatoxin and microbial strains, applied in the direction of microorganism-based methods, microorganism measurement/inspection, biochemical equipment and methods, etc., can solve problems such as environmental pollution, endangering the health of operators, and cumbersome procedures, and achieve operational Safe, Observable and Intuitive Effects

Inactive Publication Date: 2015-02-04
HARBIN INST OF TECH AT WEIHAI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] The key to biological control of aflatoxin pollution is to screen out biocontrol microbial strains that can efficiently inhibit the growth and toxin production of aflatoxin-producing bacteria. Since aflatoxin is an extremely toxic fungal metabolite, it cannot be directly observed with the naked eye after it is produced. It is realized that the production of toxins must be detected with the help of relevant instruments and analytical methods. Not only is the procedure cumbersome, but it is also likely to cause environmental pollution and endanger the health of operators. Therefore, establishing an intuitive, fast, safe, and low-cost screening can efficiently inhibit Aflatoxin microbial strain method is very important

Method used

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  • Method of intuitively screening microorganism strains for inhibiting aflatoxin
  • Method of intuitively screening microorganism strains for inhibiting aflatoxin
  • Method of intuitively screening microorganism strains for inhibiting aflatoxin

Examples

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Effect test

Embodiment 1

[0013] Example 1: Screening of marine microbial strains that inhibit aflatoxins

[0014] Prepare a solid medium plate (3 g of yeast extract, 3 g of malt extract, 5 g of peptone, 10 g of glucose, 15 g of agar, and 1000 mL of natural seawater) according to the formula in brackets, and inoculate four marine microbial strains around the plate. After cultivating at a constant temperature of 25 degrees for 2 days, inoculate the Aspergillus parasitica DM strain in the center of the plate, and observe the culture results after continuing to cultivate for 4 days. Marine microbial strains that synthesize aspergillus, such as figure 1 shown.

[0015] figure 1 It is a picture of the plate screening of marine microbial strains that can inhibit the production of orange-red drop-dispensic acid. figure 1 The strains on the positive side in the middle are marine microbial strains that can inhibit the production of orange-red scatterinic acid, that is, marine microbial strains that can inhib...

Embodiment 2

[0016] Example 2: Screening of Terrestrial Microbial Strains Inhibiting Aflatoxins

[0017] Prepare a solid medium plate according to the formula in brackets (yeast powder 5g, casein 2g, soluble starch 10g, sucrose 10g, agar 20g, tap water 1000mL), inoculate four terrestrial microbial strains around the plate, Centrally inoculate Aspergillus parasiticus DM strain, culture at a constant temperature of 32 degrees for 3-5 days, observe the culture results, any strain that can inhibit the production of orange-red scatterinic acid in the mycelia, that is, the terrestrial species that can inhibit the synthesis of aflatoxin microorganisms, such as figure 2 shown.

[0018] figure 2 It is a diagram of plate screening for terrestrial microbial strains that can inhibit the production of orange-red drop-dispensic acid. figure 2 The picture on the left in the middle is the control, which shows that a large amount of orange-red Jiangsandisnic acid is produced, and the right picture sh...

Embodiment 3

[0019] Embodiment 3: the screening of the plant endophyte strain that suppresses aflatoxin

[0020] Prepare a solid medium plate according to the formula in brackets (yeast powder 5g, glucose 20g, agar 18g, peanut seed extract 1000mL), inoculate four plant endophyte strains around the plate, and inoculate Aspergillus parasitica DM in the center of the plate Bacterial strains, after 3-5 days of constant temperature cultivation at 30 degrees, observe the culture results, any strains that can inhibit the production of orange-red scatterinic acid in mycelium are endophytic strains that can inhibit the synthesis of aflatoxin ,Such as image 3 shown.

[0021] image 3 It is a diagram of plate screening for plant endophyte strains that can inhibit the production of orange-red drop-dispensic acid. image 3 The picture on the left in the middle is the control, which shows the production of a large amount of orange-red Jiangsandisnic acid, and the right picture shows the 4 endophytic...

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Abstract

The invention relates to a method of intuitively screening microorganism strains for inhibiting aflatoxin. The method includes following steps: (1) preparing a solid culture medium plate which are suitable for growth of a marine microorganisms, a land microorganisms, an endophyte and the like microorganisms; (2) inoculating four different microorganism strains on a periphery of the plate in a spot-inoculating manner; and (3) performing cultivation in a constant temperature incubation box at 25-35 DEG C for 0-2 days; (4) inoculating an aspergillus parasiticus DM strain on the center of the plate with cultivation continuously for 3-4 days and observing mycelial growth of the aspergillus parasiticus DM strain and generation of orange norsolorinic acid through eyes. A microorganism strain, which can inhibit the generation of the orange norsolorinic acid in the mycelia of the DM strain and / or the growth of the mycelia, is the microorganism strain capable of inhibiting generation of the aflatoxin. The method of intuitively screening the microorganism strains for high-efficiently inhibiting the aflatoxin is intuitive, quick, safe and low-cost.

Description

technical field [0001] The invention relates to the technical fields of applied microorganisms, biological control and mycotoxins, and specifically relates to a method for visually screening microbial strains inhibiting aflatoxins. Background technique [0002] Aflatoxin is a toxic secondary metabolite produced by fungi such as Aspergillus flavus and Aspergillus parasiticus. It is the most toxic mycotoxin found in nature. Its acute toxicity is more than 10 times that of the highly toxic chemical potassium cyanide, and , is also highly carcinogenic, immunosuppressive and teratogenic, and is listed as a class of carcinogens by the International Agency for Cancer. Aflatoxin can widely contaminate cereal crops, oil crops, bean crops, and their processed products before harvest and during storage, which is extremely harmful to human and animal health and causes serious agricultural economic losses. Therefore, how to effectively prevent aflatoxin from contaminating agricultural p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12R1/66
Inventor 闫培生王凯王琢史翠娟
Owner HARBIN INST OF TECH AT WEIHAI
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