Method for determining the purity of nicotine by gas chromatography-mass spectrometry combined with gas chromatography
A gas chromatography mass spectrometry and gas chromatography technology, which is applied in the field of quality evaluation and determination of tobacco and tobacco products, can solve the problems of inability to distinguish nicotine and nornicotine, difficult formation of precipitates, interference with detection results, etc., and achieve reliable qualitative and quantitative results. , strong separation ability, good repeatability
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Embodiment 1
[0045] (1) Materials and instruments:
[0046] (1) Reagents: hydrochloric acid (AR, Yantai Shuangshuang Chemical Co., Ltd.); silicotungstic acid (AR, Shanghai Reagent No. 2 Factory); isopropanol (chromatographically pure, TEDIA Company, Inc.); absolute ethanol (AR, Shanghai Reagent No. 1 Factory);
[0047] (2) Instruments: Motto-Q ultrapure water device (Millipore Company); AG204 electronic balance (sensitivity: 0.0001g, Swiss METTLER TOLEDO Company); blast constant temperature drying oven (101A-2, Shanghai General Experimental Instrument Factory) GC / MS (5975, Agilent Company); GC (7890, Agilent Company); Quantitative Liquid Adder (Germany Brand Company); Pipette Gun (Tripette Company).
[0048] (2) Preparation of samples and determination of nicotine purity:
[0049] (1) Preliminary determination of the purity nic% of nicotine by GC / MS method
[0050] A. Sample preparation: Dilute nicotine sample A (long-term sample) 100 times with isopropanol as the sample to be tested, t...
Embodiment 2
[0095] Repeat the process of Example 1, select another nicotine sample B (newly prepared sample), prepare 7 parts of diluted solutions, and measure its nicotine content with GC-MS method, the spectrogram is as follows figure 2 Shown, measured value is successively 99.53%, 99.53%, 99.46%, 99.64%, 99.51%, 99.56%, 99.58%, measured average value is 99.54%, SD is 0.06; Its water content and its average value and GC / MS -Results of nicotine purity (NIC%) determined by GC method are shown in Table 1 and Table 2 respectively.
Embodiment 3
[0097] Repeat the process of Example 1, select another nicotine sample C (a long-standing sample), and prepare 4 parts of diluted solutions, and measure its nicotine content by GC-MS method, and the spectrogram is as follows: image 3 As shown, the measured values were 99.05%, 99.01%, 99.06%, and 99.01% in turn, the measured average value was 99.03%, and the SD was 0.03. The water content and its average value and the results of nicotine purity (NIC%) determined by GC / MS-GC are shown in Table 1 and Table 2 respectively.
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