A fluorescent negative staining method for detecting living cells

A negative staining method and live cell technology, which is applied in the direction of fluorescence/phosphorescence, preparation of test samples, material excitation analysis, etc., can solve the problems of cell loss of viability, inapplicability of sampling and detection of precious cell samples, and impossibility of dynamic observation, etc. Achieve the effect of maintaining vitality and easy and convenient dyeing
CN104345052BActive Publication Date: 2017-10-20江苏禄亿生生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
江苏禄亿生生物科技有限公司
Publication Date
2017-10-20

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Abstract

The invention discloses a method for detecting living cells by fluorescent negative staining. Fluorescent staining reagents stain whole cells, and when cells phagocytize opaque nano-particles, the nano-particles are enriched in the phagocytic body. Cut off the detection light so that it appears as a fluorescent negative staining area on the fluorescence image. According to the size of the negative staining area, the phagocytosis rate of the cells to the nanoparticles can be easily judged without affecting the activity of the cells. The CFSE fluorescence negative staining method of the cell labeling detection method of the present invention can be used for the detection of labeling rate and labeling amount after nano-iron-labeled cells, and for evaluating the pharmacodynamics and pharmacokinetics analysis of nano-iron-type new drugs, and the method Cells do not need to be fixed, CFSE staining is simple and convenient, and maintains the viability of cells.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for detecting cell markers, in particular to a method for dynamically detecting phagocytosis of superparamagnetic iron oxide nanoparticles by a single living cell. Background technique

[0002] With the rapid development of nanomaterial research, cell magnetic labeling has become a research hotspot in the field of imaging in recent years. Superparamagnetic iron oxide (SPIO) nanoparticles (superparamagnetic iron oxide, SPIO) is a new type of magnetic resonance contrast agent, added to the cell culture system in vitro, can be taken up by cells, and can be used for in vivo tracing after transfusion. Observing the migration and colonization of transplanted cells in various damage repair studies has been widely used in the research of mesenchymal stem cells, umbilical cord blood stem cells, and neural stem cells. SPIO is safe to use at a certain concentration, does not affect th...

Claims

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