Method for determining nonvolatile organic acid in tobacco
A non-volatile, organic acid technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of fast measurement speed, good repeatability and high reliability
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[0020] Example 1
[0021] A method for determining non-volatile organic acids in tobacco, the non-volatile organic acids mainly involved are specifically one or a combination of the following: oxalic acid, malonic acid, succinic acid, lauric acid, tridecanoic acid , Malic acid, pentadecanoic acid, palmitic acid, citric acid, stearic acid, oleic acid, linoleic acid, linolenic acid; the tobacco specifically includes one or a combination of the following: burley tobacco leaves, flue-cured tobacco Tobacco leaves, oriental tobacco leaves, tobacco stems, flakes, expanded tobacco shreds, blended tobacco shreds and flue-cured tobacco shreds; the specific requirements of the method for determining non-volatile organic acids in tobacco are as follows:
[0022] First extract the tobacco sample to obtain the corresponding treatment solution. The extraction process is as follows: pulverize the tobacco sample (the volume and mass are not required here because of the ratio behind) and place it in...
Example Embodiment
[0026] Example 2
[0027] The specific requirements of the method for determining non-volatile organic acids in tobacco are as follows:
[0028] 1. Materials and instruments: sulfuric acid (analytical purity, Kaifeng Fangjing Chemical Reagent Co., Ltd.); methanol (purity greater than 99.9%, Tianjin Siyou Fine Chemical Co., Ltd.); adipic acid (purity greater than 99.8%, Zhengzhou, China Paini Chemical Reagent Factory); oxalic acid (purity greater than 99.5%, Kaifeng Chemical Reagent Factory); malonic acid (purity greater than 98%, Shanghai Shanpu Chemical Co., Ltd.); succinic acid (purity greater than 99.5%, Shanghai Pharmaceutical Group Shanghai Chemical Reagent Company); Lauric acid (purity greater than 99.5%, ACROS); Tridecanoic acid (purity greater than 99.9%, MERCK); Malic acid (purity greater than 99.5%, Shanghai Pudong Chemical Reagent Factory, China); Pentadecanoic acid (Purity greater than 99%, ACROS); palmitic acid (purity greater than 99.5%, China National Pharmaceutical...
Example Embodiment
[0040] Example 3
[0041] The method for determining non-volatile organic acids in tobacco, for specific requirements, refer to Examples 1 and 2, in addition to the following requirements: accurately weigh about 1.0000g sample of Yunnan flue-cured tobacco C3F into a double-layer composite bag, and add The volume of the sulfuric acid-methanol solution is 35mL, the concentration of the sulfuric acid-methanol solution is 12.0%, and then 1mL adipic acid internal standard is added to dissolve it. The double-layer composite bag is heat-sealed and placed in the ultra-high pressure processing chamber. After 750MPa pressure treatment, The time is 12 minutes. Take 10 mL of the treatment solution in a 125 mL separatory funnel, add 20 mL of saturated NaCl solution, extract with 10 mL of dichloromethane each time, extract 6 times and combine the extracts, add 5.0 g of anhydrous sodium sulfate and dry overnight. The extract was determined by gas chromatography. The internal standard curve me...
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