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Method for determining nonvolatile organic acid in tobacco

A non-volatile, organic acid technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of fast measurement speed, good repeatability and high reliability

Inactive Publication Date: 2015-02-11
HONGTA LIAONING TOBACCO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no relevant reports on the determination of tobacco organic acids under ultra-high pressure conditions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for determining non-volatile organic acids in tobacco, the non-volatile organic acids mainly involved are specifically one or a combination of the following: oxalic acid, malonic acid, succinic acid, lauric acid, tridecanoic acid , malic acid, n-pentadecanoic acid, palmitic acid, citric acid, stearic acid, oleic acid, linoleic acid, linolenic acid; the tobacco specifically includes one or a combination of the following: Burley tobacco leaves, flue-cured tobacco Tobacco leaves, oriental tobacco leaves, tobacco stems, flakes, expanded shredded tobacco, mixed shredded cigarettes, and flue-cured shredded cigarettes; the specific requirements for the method for determining non-volatile organic acids in tobacco are as follows:

[0022] First extract the tobacco sample to obtain the corresponding treatment solution. The extraction process is as follows: crush the tobacco sample (because there is a ratio later, so there is no need for volume and mass here) and put it in...

Embodiment 2

[0027] The specific requirements of the method for measuring non-volatile organic acids in tobacco are as follows:

[0028] 1. Materials and instruments: sulfuric acid (analytical grade, Kaifeng Fangjing Chemical Reagent Co., Ltd.); methanol (purity greater than 99.9%, Tianjin Siyou Fine Chemicals Co., Ltd.); adipic acid (purity greater than 99.8%, Zhengzhou, China Piney Chemical Reagent Factory); oxalic acid (purity greater than 99.5%, Kaifeng Chemical Reagent General Factory); malonic acid (purity greater than 98%, Shanghai Shanpu Chemical Co., Ltd.); succinic acid (purity greater than 99.5%, Shanghai Pharmaceutical Group Shanghai Chemical Reagent Company); lauric acid (purity greater than 99.5%, ACROS); n-tridecanoic acid (purity greater than 99.9%, MERCK); malic acid (purity greater than 99.5%, Shanghai Pudong Chemical Reagent Factory); pentadecanoic acid (purity greater than 99%, ACROS); palmitic acid (purity greater than 99.5%, China Pharmaceutical Group Shanghai Chemica...

Embodiment 3

[0041] The method for measuring non-volatile organic acids in tobacco requires specific reference to Examples 1 and 2, and the following requirements: Accurately weigh about Yunnan flue-cured tobacco C3F1.0000g sample and place it in a double-layer composite bag, add The volume of the sulfuric acid-methanol solution is 35mL, the concentration of the sulfuric acid-methanol solution is 12.0%, and then 1mL of adipic acid is added for internal standard dissolution. The time is 12 minutes. Take 10 mL of the treatment liquid in a 125 mL separating funnel, add 20 mL of saturated NaCl solution, extract with 10 mL of dichloromethane each time, extract 6 times and combine the extracts, add 5.0 g of anhydrous sodium sulfate and dry overnight. The extract was determined by gas chromatography. The internal standard standard curve method was used to quantify the gas chromatographic results, and the measured value was 162.61 mg / g.

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Abstract

A method for determining non volatile organic acids in tobacco is as below: first extracting treatment liquid corresponding to a tobacco sample: crushing the tobacco sample and placing the sample in a food grade double-layer composite bag, adding 25-50ml of sulfuric acid methanol solution and 1ml of 2.0 mg / ml adipic acid internal standard solution in every 1.0000g of the tobacco sample; then conducting heat sealing on the double-layer composite bag and pressure treatment under 600-900 MPa for 10-15 min to obtain an ultrahigh pressure treatment liquid; then taking 10ml of the ultrahigh pressure treatment liquid, adding 20-30 ml of saturated NaCl solution, extracting with dichloromethane for 6 times, merging the extraction liquid, and adding 3.5 g of anhydrous sodium sulfate for drying; and determining the extraction liquid by gas chromatography to confirm the content of nonvolatile organic acids in tobacco. The method for determining nonvolatile organic acid in tobacco is carried out under ambient temperature, and has the advanategs of fast detection speed, no side effect, the determination value close to the theoretical value, good repeatability and high reliability.

Description

Technical field: [0001] The invention relates to the technical field of extraction and measurement of tobacco components, and in particular provides a method for measuring non-volatile organic acids in tobacco. Background technique: [0002] In the prior art, non-volatile organic acids are one of the main chemical components affecting the sensory quality of tobacco, especially malic acid, oleic acid, linoleic acid and malonic acid. The aroma quality and aroma quality have a great influence. It can adjust the pH value of tobacco to improve the taste, and the pH value of tobacco leaves expresses the acid-base balance state of the organic and inorganic components of tobacco leaves, which can reflect the smoke intensity (nitrogenous alkalis) and irritation, and can also indirectly reflect the tobacco Aromaticity of the air. At present, there are various methods for measuring the content of non-volatile organic acids in tobacco, and the results are also different. Each has its ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 邱宝平刘晓旭王政郭连民郭鹏张峻松
Owner HONGTA LIAONING TOBACCO
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