A Resonance Rayleigh Scattering Spectrometry Method for Determination of Tea Polyphenols
A technology of resonance Rayleigh scattering and tea polyphenols, which is applied in the field of analytical chemistry, can solve problems such as no report on the analysis method of tea polyphenols resonance Rayleigh scattering spectroscopy, and achieve the effects of high sensitivity, good selectivity, and easy operation
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[0017] Determination of tea polyphenols by resonance Rayleigh scattering spectrometry comprises the following steps:
[0018] (1) Preparation of tea polyphenol standard solution system: Take a graduated test tube, pipette 5, 100, 250, 400, 500 μL 0.1 mg / mL tea polyphenol standard solution into different test tubes, and then add 150 μL 0.2 mmol / L pH5. 0 HAc-NaAc buffer solution, 100 µL 0.01 mol / L ammonium molybdate solution, 400 µL 15 µg / mL carbon nanoparticle solution, dilute to 2.0 mL with twice distilled water, mix well, let stand for 10 min, and make multiple Tea polyphenol standard solution system;
[0019] (2) Prepare a blank control solution system: prepare a blank control solution system by the method of step (1) without adding tea polyphenol standard solution;
[0020] (3) Take an appropriate amount of tea polyphenol standard solution system and blank control solution system prepared according to steps (1) and (2), respectively, put them in a cuvette, and scan the exc...
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