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A Resonance Rayleigh Scattering Spectrometry Method for Determination of Tea Polyphenols

A technology of resonance Rayleigh scattering and tea polyphenols, which is applied in the field of analytical chemistry, can solve problems such as no report on the analysis method of tea polyphenols resonance Rayleigh scattering spectroscopy, and achieve the effects of high sensitivity, good selectivity, and easy operation

Inactive Publication Date: 2017-03-29
GUANGXI NORMAL UNIV
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Problems solved by technology

The analysis and determination methods of tea polyphenols mainly include folin phenol colorimetry (new national standard method), atomic absorption method, near-infrared spectroscopy, electrochemical analysis, high performance liquid chromatography, capillary electrophoresis, etc., but no tea polyphenols Report on Resonance Rayleigh Scattering Spectroscopic Analysis Method of Phenol

Method used

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  • A Resonance Rayleigh Scattering Spectrometry Method for Determination of Tea Polyphenols

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Embodiment

[0017] Determination of tea polyphenols by resonance Rayleigh scattering spectrometry comprises the following steps:

[0018] (1) Preparation of tea polyphenol standard solution system: Take a graduated test tube, pipette 5, 100, 250, 400, 500 μL 0.1 mg / mL tea polyphenol standard solution into different test tubes, and then add 150 μL 0.2 mmol / L pH5. 0 HAc-NaAc buffer solution, 100 µL 0.01 mol / L ammonium molybdate solution, 400 µL 15 µg / mL carbon nanoparticle solution, dilute to 2.0 mL with twice distilled water, mix well, let stand for 10 min, and make multiple Tea polyphenol standard solution system;

[0019] (2) Prepare a blank control solution system: prepare a blank control solution system by the method of step (1) without adding tea polyphenol standard solution;

[0020] (3) Take an appropriate amount of tea polyphenol standard solution system and blank control solution system prepared according to steps (1) and (2), respectively, put them in a cuvette, and scan the exc...

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Abstract

The invention discloses a resonance Rayleigh scattering spectra method measuring tea polyphenol simply and quickly. The method comprises the following steps: (1) preparing a standard tea polyphenol solution system; (2) preparing a blank comparison solution system; (3) respectively measuring resonance Rayleigh scattering peak intensity of the standard tea polyphenol solution system and the blank comparison solution I standard and I blank; calculating Delta I, which is equal to I blank minus I standard; (4) drawing a working curve according to the relation between the Delta I and the tea polyphenol density; (5) measuring a measured sample; calculating Delta I sample, which is equal to I blank minus I sample; (6) checking the working curve in the step (4) according to the measured Delta I sample of the sample to calculate the content of tea polyphenol in the measured object. The measuring method is simple in instrument, quick in operation, high in sensitivity, and good in selectivity.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a resonance Rayleigh scattering spectrometry method for determining tea polyphenols. Background technique [0002] Tea originated in China and spread throughout the world. It is one of the three major beverages in the world. Tea is one of the traditional health drinks of the Chinese nation, and its main medicinal ingredients are tea polyphenols, that is, polyhydric phenols. Tea polyphenols have anti-oxidation and good pharmacological effects, high-efficiency anti-cancer, anti-virus, anti-aging, anti-radiation, anti-mutation, scavenging free radicals, strengthening the heart and diuresis, stimulating the central nervous system, lowering blood sugar and blood lipids, and treating the heart Vascular disease, antibacterial and enzyme inhibition, prevention of arteriosclerosis, anti-caries tooth protection and other functions. The analysis and determination methods of tea polyphe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 梁爱惠王耀辉刘庆业蒋治良
Owner GUANGXI NORMAL UNIV
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