Unlock instant, AI-driven research and patent intelligence for your innovation.

A method of cultivating fast breeding for a fox -tail orchid sprout tissue

A tissue culture and orchid bud technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of difficult to achieve plants with consistent traits, unfavorable development and utilization of foxtail orchid, large differences between individuals, etc. The effect of short cycle, low investment and low production cost

Inactive Publication Date: 2016-08-24
ENVIRONMENTAL HORTICULTURE RES INST OF GUANGDONG ACADEMY OF AGRI SCI
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The offspring of Foxtail Orchid cultivated by this technology have a high degree of separation of traits and large differences among individuals, making it difficult to achieve plants with consistent traits, which is not conducive to the development and utilization of Foxtail Orchid in the flower market.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035]Select young flower buds with a length of 2-3 cm, rinse them with tap water, place them on an ultra-clean workbench and soak them in 75% alcohol for 30 seconds, then sterilize them with 0.1% HgCl2 solution for 8 minutes, and rinse them with sterile water 5 times Finally, blot the remaining water with sterile filter paper, cut out 0.5-1.0 cm long bud tips and inoculate them on the induction medium of MS+TDZ (thiadiazole phenylurea) 0.1㎎ / L+10.0% coconut milk, and cultivate for 30 After ~60 days, the buds turn green and enlarge, forming bulbous stems. The medium contains sugar 30g / L, agar 0.7%, pH value 5.5-5.8. The light intensity is 1500-2000lx, the light is 12 hours / day, and the temperature is 25-28°C. Use the bud points obtained from bud tip culture to transfer to cluster bud proliferation medium: 1 / 2MS+6-BA (6-benzylaminoadenine) 1.0㎎ / L+10.0% coconut water, cultivate for 90-120 days, and obtain a large number of The clustered buds can reach 4.5. The culture conditio...

Embodiment 2

[0037] Select 2-3 cm long young flower buds, rinse them with tap water, put them on a clean bench and soak them in 75% alcohol for 30 seconds, then sterilize them with 0.1% HgCl2 solution for 9 minutes, and rinse them with sterile water 7 times Finally, blot the remaining water with sterile filter paper, cut out 0.3-0.5 cm long bud tips and inoculate them on the induction medium of MS+TDZ (thiadiazole phenylurea) 0.15㎎ / L+15.0% coconut milk, and cultivate for 30 After ~60 days, the buds turn green and enlarge, forming bulbous stems. The medium contains sugar 30g / L, agar 0.7%, pH value 5.5-5.8. The light intensity is 1500-2000lx, the light is 12 hours / day, and the temperature is 25-28°C. Use the bud points obtained from bud tip culture to transfer to cluster bud proliferation medium: 1 / 2MS+6-BA (6-benzylaminoadenine) 2.0㎎ / L+15.0% coconut water, culture for 90-120 days, and obtain a large number of clustered buds, the multiplication factor can reach 6.0. The culture conditions...

Embodiment 3

[0039] Select young flower buds with a length of 2-3 cm, rinse them with tap water, and place them on an ultra-clean workbench, soak them in 75% alcohol for 30 seconds, then sterilize them with 0.1% HgCl2 solution for 10 minutes, and rinse them with sterile water 8 times Finally, blot the remaining water with sterile filter paper, cut out 0.3-0.5 cm long bud tip and inoculate it on the induction medium of MS+TDZ (thiadiazole phenylurea) 0.2㎎ / L+20.0% coconut milk, and cultivate for 30 After ~60 days, the buds turn green and enlarge, forming bulbous stems. The medium contains sugar 30g / L, agar 0.7%, pH value 5.5-5.8. The light intensity is 1500-2000lx, the light is 12 hours / day, and the temperature is 25-28°C. Use the bud points obtained from bud tip culture to transfer to cluster bud proliferation medium: 1 / 2MS+6-BA (6-benzylaminoadenine) 3.0㎎ / L+20.0% coconut water, culture for 90-120 days, and obtain a large number of The clustered buds can reach a multiplication factor of 7...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a culture and rapid propagation method for rhynchostylis gigantea shoot apices. A complete plant is obtained by performing induced culture on shoots, performing multiplication culture on cluster shoots, performing subculture on the cluster shoots, and performing rooting culture in sequence; the complete plant can be taken out of a bottle for performing hardening-seedling and transplanting. The method disclosed by the invention is easy to operate, low in production cost and pollution-free; scale production can be realized. According to the rhynchostylis gigantean seedlings cultured by the method, the hereditary character is stable; the characters of parents are retained; the method has a lot of advantages of immutability, low investment, high yield, short period and the like.

Description

technical field [0001] The invention belongs to the technical field of tissue culture rapid propagation, and in particular relates to a method for rapid propagation of foxtail orchid bud tip tissue culture. Background technique [0002] Foxtail Orchid (Rhynchostylis gigantea), also known as Rhynchostylis gigantea, Hainan Rhynchostylis gigantea, is a perennial epiphytic plant of the Orchidaceae Rhynchostylis gigantea, with thick leathery leaves, dark green, and variable flower colors, including white, pink, and orange , crimson and other colors, with a strong fragrance, blooming during the Spring Festival, the flowering period is long, very popular. At present, domestic and foreign researches on the rapid propagation technology of Foxtail Orchid mainly focus on the formation of callus and protocorm by seed germination, and then carry out differentiation, strong seedling and rooting culture to obtain complete plants. The progeny of the foxtail orchid cultivated by this techno...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈和明吕复兵朱根发赵超艺
Owner ENVIRONMENTAL HORTICULTURE RES INST OF GUANGDONG ACADEMY OF AGRI SCI