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Method for recycling protein from supernatant obtained from edible fungus through polysaccharide extraction and alcohol precipitation

A polysaccharide extraction and supernatant technology, applied in the field of protein recovery, can solve the problems of high sewage treatment cost and insufficient protein recovery and utilization, and achieve the effects of reducing treatment cost, rich nutrition and low investment cost.

Inactive Publication Date: 2015-03-11
庆元县金源真菌多糖制品有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The invention provides a method for recovering protein from the alcohol precipitation supernatant extracted from edible fungus polysaccharides, so as to solve the problem that the alcohol precipitation supernatant of edible fungus polysaccharides is generally used as waste liquid, the recovery and utilization of protein in it is insufficient, and the cost of sewage treatment is high. advanced questions
[0009] Aiming at the problems that the alcohol-precipitated supernatant during the extraction of edible fungus polysaccharides is generally used as waste liquid, the recovery and utilization of protein in it is insufficient, and the cost of sewage treatment is high, the patent of the present invention adopts ultrafiltration concentration to remove impurities, isoelectric point precipitation and spray drying Efficient recovery of high-quality protein components from edible fungus polysaccharide extraction waste liquid by other methods

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  • Method for recycling protein from supernatant obtained from edible fungus through polysaccharide extraction and alcohol precipitation
  • Method for recycling protein from supernatant obtained from edible fungus through polysaccharide extraction and alcohol precipitation

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Embodiment 1

[0029] Take 1Kg of mushroom fruiting bodies, add 30L of water, heat and boil for reflux to extract for 2h, centrifuge at 5000r / m to remove slag, and heat and concentrate the supernatant extract to 25% of the original volume, and add 4 times the volume of the obtained extract concentrate to a volume fraction of 95% Ethanol precipitation, centrifugation at 10000r / m, take the supernatant, and use a 3KD molecular weight cut-off ultrafiltration membrane to carry out pressurized ultrafiltration, the pressurized pressure is 5bar, to obtain a concentrated solution, adjust the pH value with 0.1mol / L hydrochloric acid 4.3. Stir for isoelectric point precipitation, then centrifuge at 10,000 r / min, add 1 L of distilled water to 72.5 g of the obtained protein precipitate, stir and disperse, and spray dry to obtain 68.3 g of mushroom protein. The process conditions of spray drying are: the temperature of the feed port is 160-180°C, and the temperature of the discharge port is 70-90°C.

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Embodiment 2

[0034] Take 1 kg of Grifola frondosa fruiting bodies, add 30 L of water, heat and boil for reflux to extract for 4 hours, centrifuge at 5000 r / m to remove slag, and heat and concentrate the supernatant extract to 25% of the original volume, and add 4 times the volume of the obtained extract concentrate to a volume fraction of 95 % ethanol alcohol precipitation, 10000r / m centrifugation, take the supernatant, and use a 5KD molecular weight cut-off ultrafiltration membrane to carry out pressurized ultrafiltration, the pressurized pressure is 4bar, to obtain a concentrated solution, adjust the pH value to 4.6, and stir for isoelectricity Spot the precipitate, then centrifuge at 10000r / min, add 78.9g of the obtained protein precipitate to stir and disperse with 1L of distilled water, and spray dry to obtain 72.6g of grifola frondosa protein. The process conditions of spray drying are: the temperature of the feed port is 160-180°C, and the temperature of the discharge port is 70-90°C...

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Abstract

The invention discloses a method for recycling protein from supernatant obtained from edible fungus through polysaccharide extraction and alcohol precipitation. The method comprises the following steps: taking the supernatant which is obtained from sporocarp of the edible fungus through the polysaccharide extraction and the alcohol precipitation; performing pressurization and ultrafiltration by adopting an ultrafiltration membrane with the cut-off molecular weight of 3-8 KD, wherein the pressure of the pressurization is 3-5 bar; performing the ultrafiltration to get a concentrated solution; adjusting the pH value to 4.0 to 5.0; mixing and performing the isoelectric precipitation; centrifuging; adding distilled water into the obtained protein precipitate, mixing, dispersing, spraying and drying, so as to obtain the recycled protein of the edible fungus. According to the method, the recycling rate of the protein is more than 70%, the content of the protein in samples is more than 70%, and the total sugar content is about 20%. Through the amino acid analysis, the nutrition of the recycled protein is rich. The treating cost of the waste alcohol precipitation liquid is effectively reduced when the edible fungus are processed through the polysaccharide extraction, and the benefit is increased through the integrated utilization.

Description

(1) Technical field [0001] The invention relates to a method for recovering protein from the alcohol precipitation supernatant extracted from edible mushroom polysaccharides. (2) Background technology [0002] Edible fungi are large fungi that humans can eat, such as shiitake mushrooms and frondosa frondosa. my country is rich in edible fungi resources, known to be close to 2,000 species. According to statistics, the current output of edible fungi in my country is about 20 million tons, accounting for more than 75% of the world's total, ranking first in the world's output. Edible fungus food integrates nutrition, functionality, delicacy and safety, and is recommended by nutritionists as one of the top ten healthy foods. Edible fungi are rich in nutrients, which mainly include polysaccharides, proteins, polypeptides, triterpenes, adenosine, etc., and are also rich in vitamins and minerals. [0003] Among them, the edible fungus polysaccharide is a kind of active polysacchar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/30
Inventor 杨开方伦叶帮伟
Owner 庆元县金源真菌多糖制品有限责任公司
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