Preparation technology of anti-rabies virus chicken yolk antibody

Abstract

A preparation technology of an anti-rabies virus chicken yolk antibody comprises the steps of immune inoculated vaccine chicken selection, immune inoculated vaccine dosage determination, immunization program, yolk separation method and skill, yolk antibody water-soluble ingredient liquid WSF preparation, yolk antibody separating extraction, yolk antibody extract product aseptic treatment, PEG solution preparation and dialysis bag treatment. The yolk antibody (IgY) is an easily available and cheap polyclonal antibody, has a good antivirus effect and has no toxic side effects, and the purified IgY is a modern ideal immunopotentiator and treatment agent, is convenient to prepare, is safe and reliable, is prepared through immunizing birds with a rabies vaccine, through generating an anti-rabies virus polyclonal antibody by bird B lymphocytes under the stimulation of rabies viruses and through transferring the anti-rabies virus polyclonal antibody to yolk, can be used for preventing and treating people rabies, and can be especially used for prevention of dog exposure high risk people and emergency prevention after dog biting.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation process of an anti-rabies virus egg yolk antibody. Background technique [0002] Rabies, commonly known as mad dog disease, also known as hydrophobia, is an acute viral infectious disease that affects the central nervous system. The pathogen is rabies virus. Rabies virus usually enters the bite wound through the venomous saliva of a rabid animal, or is infected through mucous membrane contact. The highly neurotropic rabies virus replicates in muscle tissue, enters the peripheral nerves, enters the spinal cord from the peripheral nervous system, and finally ascends to the brain. After spreading in the central nervous system (CNS), the virus is "centrifuged" from the CNS along the nerves The direction is passed back to various organs including the salivary glands, where it is discharged into saliva and then spread to the next victim via a bite wound or mucous membranes c...

AI Technical Summary

Problems solved by technology

[0003] Infected persons who have not received rabies vaccine immunization will almost certainly die when neurological symptoms appear. After vaccination, it will take a certain time for the body to induce anti-rabies virus antibodies. Failure to immunize in time will lead to the spread of rabies virus in the nervous system and cause death; In addition, passive immunization is carried out by injection of rabies immunoglobulin. The rabies immunoglobulin currently on the market is relatively expensive and is not suitable for application in the general population.