Method for preparing soybean oil by aqueous enzymatic method and application thereof

A technology of soybean oil and water enzymatic method, which is applied in the direction of fat oil/fat production, fat generation, fat oil/fat refining, etc., can solve the problem of unused extraction and processing of thawed emulsion oil, achieve the protection of nutritional value and shorten the oil extraction time , High oil extraction efficiency

Active Publication Date: 2015-03-25
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the application of the high-voltage electrostatic thawing method is mainly limited to the thawing of frozen meat and other frozen foods, and has not been applied in the thawing emulsion and oil extraction and processing.

Method used

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  • Method for preparing soybean oil by aqueous enzymatic method and application thereof
  • Method for preparing soybean oil by aqueous enzymatic method and application thereof
  • Method for preparing soybean oil by aqueous enzymatic method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038]The present embodiment provides a kind of soybean oil extraction method with traditional freezing and thawing treatment, concrete operation is as follows:

[0039] After crushing the peeled soybeans, extruding and puffing is performed at a sleeve temperature of 95°C, a die hole diameter of 18mm, a screw speed of 100r / min, and a material moisture content of 14% to obtain puffed products, which are crushed and mixed with Mix with water to obtain a mixed solution, and add 3% Alcalase alkaline to the mixed solution under the conditions that the liquid-to-material ratio is 6:1 (mL:g, the same below), the enzymolysis temperature is 55°C, and the enzymolysis pH is 9. Protease hydrolyzed for 3 hours to obtain the enzymatic solution, freezing at -20°C for 15 hours, thawing the frozen enzymolyzed solution at 60°C for 2 hours, and thawing the enzymatically Centrifuge to obtain soybean oil.

Embodiment 2

[0041] The present embodiment provides an example of soybean oil prepared by the method of the present invention, and the specific operation process is as follows:

[0042] After crushing the peeled soybeans, extruding and puffing is carried out at a sleeve temperature of 95°C, a die hole diameter of 18mm, a screw speed of 110r / min, and a material moisture content of 15% to obtain puffed products, which are crushed and mixed with Mix water to obtain a mixed solution. At a liquid-material ratio of 6.5:1, an enzymolysis temperature of 55°C, and an enzymolysis pH of 9.5, add 2.5% Alcalase alkaline protease to the mixture for 3.5 hours to obtain enzymolysis solution, after cooling the enzymolysis solution to room temperature, carry out liquid nitrogen freezing treatment at a freezing temperature of -196°C for 40 minutes and recover nitrogen gas, and then freeze the enzymolysis solution at an electric field strength of 200kV / m and a thawing temperature of 6°C The solution was subje...

Embodiment 3

[0044] The present embodiment provides another example of soybean oil prepared by the method of the present invention, and the specific operation process is as follows:

[0045] After crushing the peeled soybeans, extruding and puffing is carried out at a sleeve temperature of 100°C, a die hole diameter of 20mm, a screw speed of 120r / min, and a material moisture content of 16% to obtain puffed products, which are crushed and mixed with Mix water to obtain a mixed solution. When the liquid-to-material ratio is 7:1, the enzymolysis temperature is 50°C, and the enzymolysis pH is 9, add 3% Alcalase alkaline protease to the mixed solution for enzymolysis for 3 hours to obtain an enzymolysis solution , after cooling the enzymolysis solution to room temperature, freeze the enzymolysis solution with liquid nitrogen for 30 minutes at a freezing temperature of -196°C and recover nitrogen gas. Perform high-voltage electrostatic thawing treatment for 40 minutes, and centrifuge the thawed ...

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Abstract

The invention discloses a method for preparing soybean oil by an aqueous enzymatic method and an application thereof, belonging to the technical field of plant oil extraction and processing. The method disclosed by the invention comprises the following steps: after cleaning and peeling soybeans, crushing the soybeans; then, extruding the crushed soybeans; after extrusion, crushing a puffed product; then, hydrolyzing the puffed product by using alkaline protease; freezing the hydrolysate by using a liquid nitrogen refrigeration method after hydrolysis; and finally unfreezing by virtue of a high voltage electrostatic method and carrying out centrifugal separation and unfreezing to obtain the soybean oil. The method disclosed by the invention has the characteristics of being short in oil extraction time and high in total oil extraction rate. Just in the freezing and unfreezing links, 93.1% of time in the method provided by the invention can be saved and the total oil extraction rate can reach 95.36%. Meanwhile, the extracted soybean oil has relatively low peroxide value, p-fennel value and TOTOX value, and the quality of the extracted oil is better.

Description

technical field [0001] The invention relates to a method for preparing soybean oil by an aqueous enzymatic method and its application, and belongs to the technical field of vegetable oil extraction and processing. Background technique [0002] Aqueous enzymatic oil extraction is a novel biological oil extraction technology, which is safer and more reliable. The aqueous enzymatic process is based on mechanical crushing, using enzymes that can degrade the cell walls of vegetable oils, or compounding lipoproteins, lipopolysaccharides, etc. It is an enzyme with degradative effect on the body, acting on oil seeds, making the oil easy to release from the oil seeds, using the difference in affinity of non-oil components to oil and water, and using the difference in specific gravity of oil and water to separate oil and non-oil components. Compared with the traditional process, the aqueous enzymatic oil extraction technology has the characteristics of mild reaction conditions, simple...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/04
CPCC11B1/00C11B1/04C11B1/025C11B3/16C11B1/10
Inventor 江连洲李杨齐宝坤隋晓楠王中江冯红霞王欢马文君
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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