An effective method to obtain information on serum small molecule metabolites
A technology of small molecule metabolites and metabolites, applied in the direction of material separation, analysis materials, measurement devices, etc., to achieve high repeatability, mild reaction conditions, and strong stability
Active Publication Date: 2016-08-17
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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The present invention combines GC-MS, overlapping peak deconvolution and selected ion scanning technology, and there is no report on applying this method to improve the information of serum small molecule metabolites
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[0045] 1 sample collection
[0046] 32 pairs of serum samples before and after chemotherapy for oral cancer were provided by the Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine. The samples before chemotherapy were collected on the second day after the patients were admitted to the hospital, and the samples after chemotherapy were collected 2-3 weeks after the patients received chemotherapy. Each sample was taken while the patient was fasting.
[0047] 2 Serum QC sample preparation
[0048] Serum samples were thawed at room temperature, vortexed for 10s, and 100 μL of each sample was taken for subsequent processing, and 15 μL was taken out at the same time to prepare QC. After all the samples were taken, the QC mixed sample was vortexed for 5 minutes to mix well, and then divided into 100 μL QC test samples each, and all samples were stored at -80°C. The processing and instrumental analysis of QC samples was the same as that of the seru...
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The present invention discloses a method for acquiring more serum small molecule metabolite information compared with the conventional method in the case of no requirement of increase of the sample amount through a gas chromatography-mass spectrometry selected ion scan method and an overlapping peak deconvolution technology. According to the present invention, a serum quality control sample is subjected to oximation and silanization reactions, conventional analysis conditions are applied to obtain the mass spectrometry full scan data, an AMDIS treatment is performed to establish the initial selected ion scan table, the sample injection amount and the diversion ratio are changed so as to further obtain the trace metabolite information, a deconvolution software is applied to provide the newly-increased and qualitative trace metabolite character ion, the newly-increased and qualitative trace metabolite character ion is added to the initial selected ion scan table to form the final selected ion scan table, and finally the established selected ion scan table is adopted to carry out mass spectrometry data acquisition with the ion selecting mode on the sample; and the method has advantages of mild reaction conditions, good reproducibility, strong stability, strong operability, rich acquired metabolite information, high mass spectrometry signal signal-to-noise ratio, and the like.
Description
technical field [0001] The invention relates to the application fields of analytical chemistry and metabolomics, and is a method for effectively obtaining serum small molecule metabolite information based on gas chromatography-mass spectrometry selected ion scanning technology and overlapping peak deconvolution technology. technical background [0002] At present, chromatography-mass spectrometry technology is the mainstream technology for metabolomics research in complex biological systems. Among them, gas chromatography-mass spectrometry technology has efficient metabolite separation and qualitative performance. Polar small molecules with important physiological functions in physiological metabolic pathways, such as: pyruvate, lactic acid, phosphoenolpyruvate, ethanolamine, glycine, glycine, serine, etc., can be derivatized on capillary columns to achieve high efficiency separated, and were effectively detected and qualified by mass spectrometry. However, due to the conce...
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IPC IPC(8): G01N30/88G01N30/06
Inventor 许国旺叶国注尹沛源路鑫赵燕妮
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI