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Method for establishing rapid polygonatum sibiricum reproduction system

A Polygonatum chinensis and system technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve problems such as stimulating the enthusiasm of medicinal farmers to excavate, affecting the living environment of Polygonatum chinensis, shortening the cultivation time and cost, improving the efficiency of tissue culture, The effect of increasing the speed of fast reproduction

Active Publication Date: 2015-04-01
红河药王谷生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to its good medicinal effect, coupled with the increasing number of Chinese patent medicines and health care products using Rhizoma Polygonatum as raw materials, along with the improvement of living standards, people’s awareness of health care is increasing day by day, resulting in a sharp decrease in the amount of wild Rhizoma Rhizoma Polygonatum. It has been rarely found, and the price has risen accordingly. Now the price of dry tablets has risen from less than 20 yuan / kg two years ago to 60 yuan / kg now, which has stimulated the enthusiasm of drug farmers to dig, and even more Seriously affect the living environment of sealwort

Method used

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  • Method for establishing rapid polygonatum sibiricum reproduction system
  • Method for establishing rapid polygonatum sibiricum reproduction system

Examples

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Embodiment 1

[0021] A method for setting up a Polygonatum Polygonatum Rapid Propagation System, comprising the following steps:

[0022] Step 1. Clean the terminal buds on the perennial Polygonatum rhizomes or the tubers with buds obtained by separating them between nodes, soak them in 85% ethanol solution for 40 seconds, sterilize them with mercuric chloride for 35 minutes, and finally wash them repeatedly with sterile water 5 times, then cut off the edge of the terminal bud on the rhizome of Polygonatum or the edge of the tuber with bud, then sow in the induction medium, and the induction medium is: take MS-H as the basic medium, add 2.0mg / L6-benzyl adenine, 0.1mg / L naphthaleneacetic acid, 3.0mg / L gibberellin, 2.0mg / L thiadizuron, 1.0mg / L2,4-dichlorophenoxyacetic acid, 40g / L sucrose and 3.0g / L agar, adjust the pH of the induction medium to be 5.9; after cultivating for 10 days under the condition that the culture temperature is 29° C., the light intensity is 2600 lux, and the light tim...

Embodiment 2

[0027] A method for setting up a Polygonatum Polygonatum Rapid Propagation System, comprising the following steps:

[0028] Step 1. Clean the terminal buds on the perennial Polygonatum rhizome or the tubers with buds obtained by separating them between nodes, soak them in 80% ethanol solution for 60 seconds, sterilize with mercuric chloride for 45 minutes, and finally wash them repeatedly with sterile water 6 times, then cut off the edge of the terminal bud on the rhizome of Polygonatum or the edge of the tuber with bud, then sow in the induction medium, the induction medium is: take MS-H as the basic medium, add 3.0mg / L6-benzyl adenine, 0.5mg / L naphthaleneacetic acid, 2.0mg / L gibberellin, 0.5mg / L thiadizuron, 3.0mg / L2,4-dichlorophenoxyacetic acid, 40g / L sucrose and 4.0g / L agar, adjust the pH of the induction medium to be 6.3; after cultivating for 14 days under the conditions that the culture temperature is 28°C, the light intensity is 2800 lux, and the light time is 15h / d,...

Embodiment 3

[0033] A method for setting up a Polygonatum Polygonatum Rapid Propagation System, comprising the following steps:

[0034] Step 1. Clean the terminal buds on the perennial Polygonatum rhizome or the tubers with buds obtained by separating them between nodes, soak them in 85% ethanol solution for 60 seconds, disinfect them with mercuric chloride for 45 minutes, and finally wash them repeatedly with sterile water 7 times, then cut off the edge of the terminal bud on the rhizome of Polygonatum or the edge of the tuber with bud, then sow in the induction medium, and the induction medium is: take MS-H as the basic medium, add 4.0mg / L6-benzyl adenine, 0.1mg / L naphthaleneacetic acid, 3.0mg / L gibberellin, 1.0mg / L thiadizuron, 1.0mg / L2,4-dichlorophenoxyacetic acid, 30g / L sucrose and 5.0g / L agar, adjust the pH of the induction medium to be 6.0; after cultivating for 7-15 days under the conditions that the culture temperature is 30° C., the light intensity is 3000 lux, and the light t...

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Abstract

The invention discloses a method for establishing a rapid polygonatum sibiricum reproduction system. The method comprises the following steps: seeding terminal buds or tubers of polygonatum sibiricum into an induction medium to induce germination, transferring small polygonatum sibiricum buds into a subculture medium and a rooting medium for continuous culture in sequence, thereby obtaining tissue culture seedlings of polygonatum sibiricum, and transplanting the tissue culture seedlings into a seedbed for seedling hardening. The tissue culture method disclosed by the invention is simple and convenient, simple in step and convenient to operate, obtained polygonatum sibiricum seedlings are strong in plant, high in growing ability and applicable to large-scale production, meanwhile the culture time is effectively shortened, and the production cost is lowered.

Description

technical field [0001] The invention relates to a method for rapid propagation of plants, in particular to a method for establishing a rapid propagation system of Polygonatum. Background technique [0002] Polygonatum kingianum (Polygonatum kingianum Coll.et Hemsl.) is the underground rhizome of Polygonatum kingianum Coll.et Hemsl., a yellow metal plant of Liliaceae. . Nature is sweet, flat. Tonify qi and nourish yin, invigorate the spleen, moisten the lungs, and benefit the kidneys. For weakness of the spleen and stomach, fatigue and fatigue, dry mouth and lack of food, dry cough due to lung deficiency, insufficient essence and blood, internal heat and thirst. The medical books of the past dynasties are highly praised, and the function of [Mongolian medicine] is the same as Polygonatum Polygonatum "Mongolian medicine". [Wa Medicine] Signa: Rhizome cures pulmonary tuberculosis, dry cough without phlegm, chronic illness, dry mouth, fatigue, diabetes, high blood pressure "...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 霍丽妮陈睿苏钛李培源苏炜王杰鹏
Owner 红河药王谷生物科技有限公司
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