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Method for producing mycoprotein feed by utilizing sweet sorghum straws and/or related waste residues

A sweet sorghum straw, protein feed technology, applied in animal feed, animal feed, application and other directions, can solve the problems of low utilization efficiency of pentose, poor feed palatability, complex process, etc., to improve feed protein conversion rate and palatability. , the effect of shortening the production cycle and improving the degradation efficiency

Inactive Publication Date: 2015-04-01
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1) The efficiency of the existing steam explosion pretreatment technology for sweet sorghum stalks needs to be further improved. The use of urea and other ammonia explosion technologies is likely to cause secondary pollution, and the loss of ammonia gas is not conducive to the use of microorganisms as nitrogen sources again;
[0006] 2) Optimizing the combination of fermentation microbial strains, the key problems are the low utilization efficiency of pentose sugar in straw, low conversion efficiency of true protein in straw and poor palatability of feed;
[0007] 3) Solve the problems of long secondary fermentation production cycle, complex process, and large amount of spores in the late stage of fungal fermentation

Method used

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  • Method for producing mycoprotein feed by utilizing sweet sorghum straws and/or related waste residues
  • Method for producing mycoprotein feed by utilizing sweet sorghum straws and/or related waste residues
  • Method for producing mycoprotein feed by utilizing sweet sorghum straws and/or related waste residues

Examples

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Effect test

Embodiment 1

[0029] A method for producing bacterial protein feed by utilizing sweet sorghum stalks and / or related waste residues, the process flow is as follows figure 1 As shown, the specific steps are as follows:

[0030] 1) Pretreatment: Cut fresh sweet sorghum stalks into short pieces of about 0.1 cm with a hay cutter, add dilute sulfuric acid (concentration 0.1%), pre-soak for 8 hours, and the water content reaches 60%, and blast with a steam explosion device. The blasting conditions are: temperature 210°C, pressure 2kPa, pressure maintenance time 90s;

[0031] 2) Koji production by solid fermentation: Inoculate Aspergillus niger and Trichoderma reesei preserved on the slant into Erlenmeyer shaker flasks containing potato dextrose agar medium, culture at 28°C and 150 rpm for two days, and the number of Aspergillus niger spores reaches : 3.8×10 9 Individuals / g, the number of Trichoderma reesei spores: 2.2×10 8 per g; the activated two kinds of bacterial liquids were inserted into the...

Embodiment 2

[0038] A method for producing bacterial protein feed by utilizing sweet sorghum stalks and / or related waste residues, the process flow is as follows figure 1 As shown, the specific steps are as follows:

[0039] 1) Pretreatment: Use a hay cutter to cut the sweet sorghum stalks after dust removal and natural air-dry storage for 3 months into short pieces of about 0.1 cm, add dilute sulfuric acid (concentration 0.3%), pre-soak for 10 hours, and the water content reaches 65% , using a steam explosion device for blasting, the blasting conditions are: temperature 200°C, pressure 2.5kPa, pressure maintenance time 100s;

[0040] 2) Solid fermentation koji production: during activation culture, the number of spores of Aspergillus niger reaches: 2.7×10 9 Individual / g, the number of Trichoderma reesei spores: 2.6×10 8 Individual / g; Others are the same as in Example 1.

[0041] 3) Seed production by liquid fermentation: during activation culture, the number of viable Candida utilis ba...

Embodiment 3

[0047] A method for producing bacterial protein feed by utilizing sweet sorghum stalks and / or related waste residues, the process flow is as follows figure 1 As shown, the specific steps are as follows:

[0048] 1) Pretreatment: add dilute sulfuric acid (concentration: 0.7%) to sweet sorghum stalk residue after squeezing the juice, pre-soak for 12 hours, and the water content reaches 70%, and use a steam explosion device to blast. The blasting conditions are: temperature 210 ° C, pressure 3 kPa, Maintain pressure for 110s;

[0049] 2) Solid fermentation koji production: during activation culture, the number of spores of Aspergillus niger reaches: 6.9×10 9 per g, the number of Trichoderma reesei spores: 4.2×10 8 Individual / g; Others are the same as in Example 1.

[0050] 3) Seed production by liquid fermentation: During activation culture, the number of viable Candida utilis: 2.5×10 8 Individual / g, the number of viable Lactobacillus casei reached: 1.8×10 8 Individual / g; Ot...

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Abstract

The invention belongs to the technical field of a protein feed, and particularly relates to a method for producing mycoprotein feed by utilizing sweet sorghum straws and / or related waste residues. The method comprises the following steps: firstly, adding diluted acid for pre-soaking the sweet sorghum straws and / or related waste residues, and performing steam explosion; preparing aspergillus niger and trichoderma reesei mixture by using a shallow tray solid fermentation method, and respectively culturing candida utilis and lactobacillus casei by using a liquid submerged fermentation method; spreading 0.1-1% of urea into the exposed sweet sorghum straws and / or related waste residues, inoculating the aspergillus niger and trichoderma reesei mixture and candida utilis respectively according to the inoculum sizes of 10-20% and 5-10%, evenly stirring, and performing normal-temperature aerobic fermentation for 6-8 days; inoculating lactobacillus casei to the aerobic-fermented straws according to the inoculum sizes of 3-10%, regulating the moisture content to be 60-70%, and packaging through a vacuum compression packaging machine and an anaerobic fermentation feed bag. The produced mycoprotein feed has the advantages that the content of crude protein reaches 21.5-26.1%, the content of coarse fibers are up to 6.4-8.3%, the coarse ash content reaches 2.3-4.7%, the palatability is strong, and the mycoprotein feed is easily absorbed and utilized by animals.

Description

technical field [0001] The invention belongs to the technical field of protein feed production, and in particular relates to a method for producing bacterial protein feed by using sweet sorghum stalks and / or related waste residues. Background technique [0002] With the rapid development of animal husbandry in my country, the demand for feed has increased greatly. However, in the world, feed production, especially feed protein production, is often in short supply. At present, domestic feed protein mainly comes from soybean meal and fish meal, and most of them rely on imports, and the price is also rising year by year. Moreover, with the negative effects brought about by global environmental pollution and the greenhouse effect, the output of traditional soybean meal and fish meal and other feed proteins shows a decreasing trend year by year. Facing the shortage of feed protein resources, the development of new alternative feed protein resources has become a worldwide proble...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/14
Inventor 宋安东杨森吕朝锋王石垒谢慧陈红歌王风芹韦伊强赵瑞晓赵宏军高德昌任天宝薛歧庚
Owner HENAN AGRICULTURAL UNIVERSITY
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