Method of in-vitro amplification and purification culture of mesenchymal stem cells

A bone marrow mesenchymal and in vitro expansion technology, applied in the field of in vitro expansion, purification and culture of bone marrow mesenchymal stem cells, can solve the problems of loss of expansion ability, large amount of bone marrow, low cell viability, etc., to reduce costs, damage reduction effect
CN104480068AInactive Publication Date: 2015-04-01黄相杰
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
黄相杰
Publication Date
2015-04-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method of in-vitro amplification and purification culture of mesenchymal stem cells in differentiation from mesenchymal stem cells to osteoblasts. A used cell culture medium comprises the following components: 5-20% of PL platelet lysate, 2-5U / ml of heparin, 5-10microgram / ml of doxycycline, and a basic culture medium DMEM or a basic culture medium AMEM. According to the method disclosed by the invention, the method comprising steps of separation and extraction of mesenchymal stem cells, cell inoculation, cell culture, cell culture fluid replacement and cell propagation are adopted, and the obtained mesenchymal stem cells are high in purity, high in in-vitro amplification capacity, and good in the capacity of differentiation to osteoblasts.
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Description

technical field

[0001] The invention relates to the technical field of collection, separation and extraction of bone marrow mesenchymal stem cells, and in vitro culture, expansion and purification of cells, in particular to a method for in vitro expansion, purification and culture of bone marrow mesenchymal stem cells. Background technique

[0002] Bone marrow mesenchymal stem cells originate from the mesoderm in the early stage of development and are non-terminal differentiated cells. This kind of cells is easy to collect, isolate and culture, and has strong self-renewal, differentiation ability and immunosuppressive advantages in vitro, so it has an important role in the fields of tissue and organ defect diseases, tissue and organ degenerative diseases, cell therapy and tissue engineering. Application prospects. In recent years, there have been many studies at home and abroad to explore the in vitro isolation and culture methods of bone marrow mesenchymal stem cells, mainl...

Claims

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