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Broken-wall saccharomyces cerevisiae culture with bean pulp as base material and preparation method of culture

A technology of Saccharomyces cerevisiae and culture, applied in the field of solid-state fermentation and enzymolysis, can solve the problems of unseen, low production cost, high protein digestion and absorption rate, etc., and achieve the effects of good palatability, low cost, and high protein digestion and absorption rate

Inactive Publication Date: 2015-04-08
湖北邦之德牧业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using soybean meal as the base material for preparing yeast culture to obtain yeast high-density fermented soybean meal, and then further enzymatic hydrolysis and autolysis to obtain yeast culture products rich in polypeptides, has not yet seen an effective prior art solution; people It is expected that this yeast culture has the advantages of both soybean polypeptide and yeast culture. It is an upgraded product of fermented soybean meal, including high immune activity, good palatability, high protein digestion and absorption rate, and low production cost. The usage can reach 10 %, is a high-quality feed vegetable protein conventional raw material, the effect is more significant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: It is the basic embodiment of the broken Saccharomyces cerevisiae culture using soybean meal as the base material of the present invention. A culture of broken Saccharomyces cerevisiae with soybean meal as the base material, which uses soybean meal as the base material, adds nutritional supplements and inoculates active dry yeast, and then undergoes fermentation and culture to obtain a high-density Saccharomyces cerevisiae culture; then adjusts the pH value , add compound enzyme, co-solvent and water, maintain a certain temperature to enzymatically break the wall, and the high-density Saccharomyces cerevisiae wall-breaking rate is 80% to 90%; finally, the finished product of the broken-wall Saccharomyces cerevisiae culture is obtained after drying, and its small peptide content 10% to 30%, amino acid nitrogen content is 0.5% to 2%.

Embodiment 2

[0036]Embodiment 2: is a further embodiment on the basis of Embodiment 1. Quantitative analysis of the protein distribution of different molecular weights of the broken Saccharomyces cerevisiae culture was carried out by gel filtration, and the small molecular weight proteins and small peptides with a molecular weight below 2000 Da accounted for 50% to 85% of the total protein.

Embodiment 3

[0037] Embodiment 3: is a further embodiment on the basis of Embodiment 1. The wall breaking rate of the high-density Saccharomyces cerevisiae is 85%-90%; the small peptide content of the wall-broken Saccharomyces cerevisiae culture is 15%-30%, and the amino acid nitrogen content is 1%-2%.

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Abstract

The invention relates to a broken-wall saccharomyces cerevisiae culture with bean pulp as a base material and a preparation method of the culture. The broken-wall saccharomyces cerevisiae culture is prepared by virtue of the following steps: taking the bean pulp as the base material, adding nutritional accessories and inoculating with an active dry yeast, and performing fermentation cultivation, thereby obtaining a high-density saccharomyces cerevisiae culture; next, adjusting the pH value, adding a compound enzyme, a cosolvent and water and preserving heat for enzymolysis and wall breaking; drying to obtain the broken-wall saccharomyces cerevisiae culture of which the content of small peptides is 10%-30% and the content of amino acid nitrogen is 0.5%-2%. The preparation method of the culture comprises preparing the high-density saccharomyces cerevisiae culture, performing enzymolysis and wall breaking, drying the semi-finished product and determining the finished product. The broken-wall saccharomyces cerevisiae culture with the bean pulp as the base material has the advantages of soybean peptides and yeast cultures, and is high in immunocompetence, good in palatability and high in protein digestibility; the culture is a good-quality vegetable protein feed and is capable of taking the place of animal protein feeds such as fish meal and plasma proteins; the preparation method is simple and stable in process, easy to operate and control, suitable for large industrial production and low in cost.

Description

technical field [0001] The invention relates to a polypeptide-rich Saccharomyces cerevisiae culture and a preparation method thereof, in particular to a broken-wall Saccharomyces cerevisiae culture with soybean meal as a base material and a preparation method thereof, belonging to the technical field of solid-state fermentation enzymolysis. Background technique [0002] Yeast culture, as a kind of probiotics, is composed of yeast, mainly Saccharomyces cerevisiae ( Saccharomyces cerevisiae ), under the control of modern fermentation technology, it is a product obtained by combining liquid and solid state or directly fermenting on solid medium and then processing it together with solid substrate. This product does not contain a large amount of active yeast. Yeast is only a tool in the production of yeast culture products. Whether it survives in the final product will not affect the use effect of the product. Its main active ingredients are extracellular metabolites produced b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/14C12N1/06C12R1/865
Inventor 葛向阳李俊
Owner 湖北邦之德牧业科技有限公司
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