A new type of antibacterial active peptide based on the body's own secretion

Abstract

The embodiment of the invention discloses novel antibacterial active peptide based on human autocrine. According to the novel antibacterial active peptide, SPLUNC1 protein excreted by a human body is taken as a template, a combinational design of novel antibacterial peptide is carried out by combining the molecular design and synthesis theories of antibacterial peptides (AMP) with a biological evolution conserved gene principle based on antibacterial mode sequence requirements and modification on amino acid structures and residue structures of prepotent loci, and a mature peptide synthetic technique is utilized for preparing novel antibacterial active peptide; the length of each AMP is only equal to 1 / 10 of that of primary SPLUNC1 protein, furthermore, the synthesis is easy, and the cost is low; the new synthesized AMP is derived from highly conserved amino acid sequences of the human body and has high biological stability and a proper half-life period; in vitro experiments prove that the new synthesized AMP has relatively good water solubility and broad-spectrum antibacterial and anti-fungus actions and is expected to be developed into a new antibacterial clinical drug, and hemolysis is nearly avoided.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a class of novel antibacterial active peptides secreted by the human body itself. Background technique [0002] Since the invention of antibiotics, humans have made great achievements in controlling and treating microbial infections. However, with the continuous use of antibiotics, microbial resistance has become a major problem in clinical microbial infection control, so that there is a lack of first-line drugs to inhibit or kill certain microbial bacteria. In clinical drug treatment, staphylococci and enterococci resistant to vancomycin, Gram-negative bacteria producing extended-spectrum β-lactamase, etc. have appeared, which seriously threaten human health. Therefore, the development of antibacterial drugs with novel antibacterial mechanisms is an important way to solve antibiotic resistance. [0003] Antimicrobial peptides (AMP) refer to small molecule basi...

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Problems solved by technology

However, these methods are all based on the structure of natural antimicrobial peptides. Although they can improve some of their defects, the designed molecules have the problem of insufficient structural diversity; although the combined peptide library method can well meet the requirements of molecular diversity, and can Realize the high-throughput screening of antimicrobial peptides, but the experiment requires a lot of manpower, material resources, and financial resources; the new design based on molecular simulation can realize the virtual design and efficient screening of antimicrobial peptides, and can theoretically explain the mechanism of action of antimicrobial peptides in vivo , but this method is mainly used to study the mechanism of action of antimicrobial peptides and cell membranes, and the calculation amount is relatively large

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