High value utilization method of plant oil hydrated oil foots
A technology for hydrating oily feet and vegetable oil, which is applied in recycling technology, recovery of fatty substances, recovery of fat oil/fatty acid from waste, etc. It can solve the problem of low added value and achieve the effect of increasing added value.
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Embodiment 1
[0026]The water content of soybean oil hydrated oil bottom analysis is 51.8%, the acetone insoluble matter in dry matter is 63.1%, and the rest is acetone soluble oil. Take 100 g of the oily bottom, place it in a Erlenmeyer flask, and put it in a water bath at 50° C. for 30 minutes, and add phospholipase Lecitase Ultra (Novozymes, Denmark) at 1 IU / g of the oily bottom. Leciatase Ultra is strictly a lipase, but it also has high catalytic activity for phospholipid substrates, therefore, the enzyme preparation can also be considered as a phospholipase. The reaction was stirred in a water bath at 50° C. until the acetone insoluble matter was reduced by about 20%, and the reaction was carried out for 38 hours. Take 10g of the reactant and centrifuge, the upper layer is a clear layered oil phase, and the lower layer is a heterogeneous turbidity. The weight of the upper layer oil phase is 1.43g, and the acid value is 40.2.
Embodiment 2
[0028] The water content of soybean oil hydrated oil bottom analysis is 51.8%, the acetone insoluble matter in dry matter is 63.1%, and the rest is acetone soluble oil. Take 100 g of the oil bottom, place it in a conical flask, add phospholipase Lecitase Ultra (from Novozymes, Denmark) according to 10IU / g oil bottom after 40°C water bath for 30min, and stir the reaction in a water bath at 40°C until the acetone insolubles are reduced by about 45%. The reaction was carried out for 22 hr. Take 10g of the reactant and centrifuge, the upper layer is a clearly layered oil phase, and the lower layer is a heterogeneous turbidity. The weight of the upper oil phase is 2.28g, and the acid value is 78.7.
Embodiment 3
[0030] The water content of soybean oil hydrated oil bottom analysis is 51.8%, the acetone insoluble matter in dry matter is 63.1%, and the rest is acetone soluble oil. Take 100 g of the oily bottom, place it in a conical flask, and add porcine pancreas phospholipase A2 (Sigma Company) at 10 IU / g of the oily bottom after a 65°C water bath for 30 minutes, and stir the reaction in a 65°C water bath until the acetone insolubles are reduced by about 28%. , the reaction was carried out for 6hr. Take 10g of the reactant and centrifuge, the upper layer is a clearly layered oil phase, and the lower layer is a heterogeneous turbidity. The weight of the upper oil phase is 2.16g, and the acid value is 62.6.
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