Nucleic acid membrane strip and kit for detecting gene of hereditary hearing loss

Abstract

The invention relates to the field of gene detection and particularly relates to a nucleic acid membrane strip and a kit for detecting a gene of hereditary hearing loss. The nucleic acid membrane strip comprises a substrate and specific oligonucleotide probes which are fixed on the substrate, wherein the sequences of the oligonucleotide probes are shown in SEQ ID NO: 1-42. According to another technical scheme, the invention provides a kit for detecting the gene of hereditary hearing loss. The kit comprises the nucleic acid membrane strip detecting the gene of hereditary hearing loss, a PCR reaction liquid I and a PCR reaction liquid II. The nucleic acid membrane strip and kit provided by the invention have the beneficial effects that the nucleic acid membrane strip and kit comprise eight pairs of primers capable of being efficiently and specifically amplified and 42 probes capable of being specifically hybridized. The detection sites are relatively complete, so that 21 common hearing loss sites can be simultaneously detected by using two tubes of the reaction liquid and one membrane strip.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to a nucleic acid membrane strip and a kit for genetic detection of hereditary deafness. Background technique [0002] Lesions occur in the auditory nerve and central centers at all levels in the sound transmission, sensory sound and auditory conduction pathways of the auditory system, causing auditory dysfunction and varying degrees of hearing loss, collectively referred to as deafness. Deafness is one of the most common diseases that seriously affect human health and quality of life. It is estimated that more than 700 million people in the world have moderate or above (55dB) hearing loss. According to my country's second national sample survey of disabled people in 2006, there were 27.8 million people with hearing and speech disabilities, of which 20.04 million were simply hearing disabled, accounting for 24.16% of the 82.96 million disabled people in the country. Among the hearing-...

AI Technical Summary

Problems solved by technology

[0005] Deafness detection products using genetic detection methods include Boao Bio Group's nine hereditary deafness gene detection kits (microarray chip method) and fifteen hereditary Deafness-related gene detection kit (microarray chip method), deafness gene GJB2 235delC detection kit (fluorescence PCR method) of Jinan Yingsheng Biotechnology Co., Ltd., drug-induced deafness gene mutation detection reagent of Zhihai Bioengineering (Beijing) Co., Ltd. Kit (fluorescent PCR method), four deafness gene detection kits (ARMS-PCR method) of Zhongsheng Beikong Biotechnology Co., Ltd., mitochondrial DNA A1555G mutation detection kit (PCR- Enzyme digestion method), these products have their own advantages, but they all have certain shortcomings, or they cannot be qualitative, or they are time-consuming and labor-intensive, and the equipment required is expensive. The most important thing is that most of these products are difficult to detect multiple mutations of different genes at the same time. site detection

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