Production method for extracting heparin from pig lung

Abstract

The invention relates to a production method for extracting heparin from a pig lung. The production method comprises the following steps: mincing a fresh pig lung into a slurry; carrying out heat preservation and enzymolysis on the slurry; filtering enzymolysis liquid, and collecting filter liquor; and carrying out deproteinization treatment, ultrafiltration, oxidation precipitation and freeze drying on the filter liquor to obtain a crude heparin product. The production method disclosed by the invention has the beneficial effects that the pig lung slurry can be more thoroughly and comprehensively dissolved by adopting an enzymolysis method, and the enzymolysis is terminated by using trypsin; by virtue of a vacuum freeze drying technology, the product quality is stabilized, the yield is enhanced, and the obtained crude product is few in impurity and high in valence; according to the production method, clarifying agent is adopted to replace a traditional process of removing protein through repeated ethanol precipitation, so that the removal rate is high, the usage amount of a large amount of ethanol is saved and the production cost is lowered; the production method is reasonable in process design, non-toxic, harmless and biologically green and environmental-friendly in production, easily available in pig lung raw material sources, low in production cost and suitable for large-scale industrial production.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a production method for extracting heparin from pig lungs. Background technique [0002] Heparin is an acidic mucopolysaccharide sulfate anticoagulant. In recent years, studies have proved that heparin also has the effect of lowering blood lipids. Over the years, the demand for heparin in the international market has been very strong. Because heparin is extracted and processed from animal viscera, the output is limited to a certain extent, and often cannot meet market demand. Since the late 1990s, the demand for heparin in the global market has increased significantly. The production and export of heparin in my country have increased significantly year after year, and the export volume and export value have shown a rapid growth trend. China has become the world's leading exporter of heparin raw materials. In 2001, the national export value of heparin products was 44.6 million US dol...

AI Technical Summary

Problems solved by technology

[0004] At present, the methods for domestic production of heparin are mainly salt hydrolysis and acid hydrolysis, such as Chinese invention applications with publication numbers CN101735340A, CN101649008A, CN101519459A, etc. These two methods all use pig small intestinal mucosa as raw material, and under alkaline conditions After salt hydrolysis and enzymatic hydrolysis, part of the protein is removed, then eluted by trimethylamine quaternary ammonium strong basic cation exchange resin adsorbent, and ethanol precipitation is added to the eluent to obtain the primary raw material of heparin sodium for sale. The disadvantage is that the production cycle is long Up to 48 hours, the reaction temperature is 95-100°C, the energy consumption is large, the hydrolysis is incomplete, and the yield is low. It takes 2800-3000 pieces of pig small intestinal mucosa to produce 100 million international units (IU) of crude heparin. The purity of the obtained product is low, and the crude product contains heparin It is at most 80 potency / mg; and produces a large amount of fat intestine residue and waste water, pollutes environment; Low, low yield, low titer, it is difficult to carry out large-scale production, and cannot meet the large demand of the market