Fiber bed reactor suitable for aerobic fermentation and method for producing l-ornithine
A reactor and ornithine technology, which is applied in the field of fiber bed reactors for fermenting and producing L-ornithine, can solve the problems of difficult continuous fermentation, poor adaptability, and few applications, and achieve continuous multi-batch immobilization. Fermentation production, high production efficiency and high mass transfer efficiency
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0045] A 5L stirred reactor uses glucose as a carbon source fiber bed to ferment and produce L-ornithine with immobilized cells.
[0046] Using reticular plant fiber gauze as the immobilization material, inoculate fresh Corynebacterium glitamicum1006 in the 5L first stirred reactor with 2L seed medium, and turn on the constant flow pump to make the material circulation between the first stirred reactor and the immobilization device exchange, so that the bacteria are immobilized on the immobilized column, wherein, the culture conditions are: temperature 30°C, stirring speed 400r / min, material circulation exchange speed 50ml / min, and the pH is controlled between 7 and 8 by the pH control device During the period, the aeration ratio of the stirred reactor is 1: 0.5-1.5, and the aeration ratio of the immobilized column is 1: 0.5-1.5. After culturing for 20 hours; release the seed medium in the first agitated reactor;
[0047] Add 3L of fermentation medium to the first stirred reac...
Embodiment 2
[0051] A 5L stirred reactor uses glucose as a carbon source to produce L-ornithine by fermenting immobilized cells in an airlift circulating fiber bed.
[0052] Using reticular plant fiber gauze as immobilized material, connect fresh Corynebacterium glitamicm1006 to the 5L first stirred reactor pre-loaded with 2L seed medium, and produce it through the air inlet of the first stirred reactor. The negative pressure makes the liquid circulate and exchange materials between the first stirred reactor and the immobilization device, so that the bacteria are fixed on the immobilization column. The temperature of the immobilization device is 30°C, and the first stirring reaction The stirring speed of the reactor is 350r / min, the speed of material circulation exchange is 60ml / min, the ventilation ratio of the reactor is 1:0.5~1.5, the ventilation ratio of the immobilized column is 1:0.5~1.5, and the pH is controlled by the pH control device at 6~ 8, release the seed medium in the first ...
Embodiment 3
[0057] 5L stirred reactor uses glucose as carbon source to ferment L-ornithine in batches with free cells (without external immobilization device);
[0058] Connect fresh Corynebacterium glutamicum1006 to a 500ml Erlenmeyer flask pre-installed with 100ml seed medium, cultivate it for 11h at 30°C under a shaker condition of 220r / min, and inoculate the seed solution in the pre- Cultivate in a 5L fermenter equipped with 3L of sterilized fermentation medium, culture conditions: 30°C, 400r / min, pH controlled by a pH control device between 6 and 8, and an aeration ratio of 1:0.5 to 1.5. When the residual sugar in the fermentation broth is about 10g / L, feed-fed fermentation is carried out, and the concentration of L-ornithine is 34.05g / L after fermentation for 50 hours.
[0059] Table 1. Effects of different production methods on L-ornithine production
[0060]
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More