A method for efficiently saccharifying herbal energy plants
A herbal energy plant and high-efficiency technology, applied in the field of high-efficiency saccharification herbal energy plants, can solve the problems of reducing the utilization of raw material sugar components, limited sugar concentration in hydrolyzed liquid, and high energy consumption per unit of raw material, so as to reduce water consumption and improve enzyme use efficiency , the effect of reducing water and energy consumption
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Embodiment 1
[0036] Use pennisetum as raw material, crush it to a particle size of less than 1cm, place it in an enzymolysis reaction device, add the enzymolysis solution according to the mass volume concentration of the enzymolysis raw material at 10% g / mL, and use the ratio of 20FPU / g cellulose Add cellulase, enzymolyze at pH 4.8, temperature 50°C for 24 hours, separate solid and liquid, collect enzymolysis residue and enzymolysis sugar solution; put enzymolysis residue in hydrothermal pretreatment reaction device, press Add a hydrothermal buffer solution (phosphoric acid-sodium phosphate-sodium borate) with a mass volume concentration of 10% g / mL, stir evenly, and react for 10 minutes at 180°C in a 4MPa nitrogen atmosphere, separate solid and liquid, and collect for pretreatment Residue and pretreated sugar solution; put the pretreated residue in the enzymolysis reaction device, add the above enzymatic sugar solution according to the mass volume concentration of the enzymolysis raw mater...
Embodiment 2
[0038] Use pennisetum as raw material, crush it to a particle size of less than 1cm, place it in an enzymolysis reaction device, add the enzymolysis solution according to the mass volume concentration of the enzymolysis raw material at 10% g / mL, and use a ratio of 30FPU / g cellulose Cellulase was added, and after 24 hours of enzymatic hydrolysis under the conditions of pH 4.8 and temperature 50° C., the solid and liquid were separated, and the enzymatic sugar solution was collected and recycled for use in the enzymatic hydrolysis process. The enzymatic hydrolysis raw material pretreatment conditions, enzymatic hydrolysis conditions and results in the circulation process are shown in the table below. After 4 uses of the enzymatic hydrolysis sugar liquid, the concentration of glucose in the enzymatic hydrolysis sugar liquid reaches 203.7g / L, and the concentration of xylose reaches 47.8g / L.
[0039] Table 1 Changes in sugar concentration in enzymatic sugar solution after recyclin...
Embodiment 3
[0043] Referring to Example 1 for enzymatic hydrolysis treatment, the changes in the concentration of the pretreated sugar solution brought about by the change in the number of uses of the pretreated sugar solution in the hydrothermal pretreatment reaction were investigated.
[0044]In the hydrothermal pretreatment reaction device, add a hydrothermal buffer solution (potassium dihydrogen phosphate-dipotassium hydrogen phosphate-boric acid) with a pH of 5 at a mass volume concentration of 10% g / mL and stir evenly. In the atmosphere, react for 60 minutes, separate the solid and liquid, collect the pretreated sugar solution, and recycle it for pretreatment. The recycling conditions and results are shown in the table below. After 3 uses of the pretreated sugar solution, the total xylose concentration in the pretreated sugar solution reaches 115.1g / L, the xylose concentration reaches 37.1g / L, and the glucose concentration reaches 16.8g / L.
[0045] Table 2 Changes in sugar concent...
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