Culturing method of momordica cochinchinensis

A culturing method and a technology for the soft-shelled turtle, applied in the field of plant reproduction, can solve the problems of limited number of seeds, limited reproduction number, uneven population, etc., achieve large-scale production, improve the reproduction coefficient, and solve the problem of easy drying. Effect

Inactive Publication Date: 2015-04-29
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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AI Technical Summary

Problems solved by technology

[0003] Wood softshell turtles are often propagated by seeds and root tubers; however, the number of seeds is limited, the germination and seedling rate is low, and because of dioeciousness, the genetic background of the offspring of seedlings is heterozygous, the population is uneven, and it is difficult to guarantee the flowering and fruiting of the year; root tubers Propagation is usually cut into several small pieces according to the number of bud eyes on the tuber root, each piece has at least one bud eye, buried in the sowing hole, one piece for each hole, generally can bloom and bear fruit in the same year, faster than seed propagation, but the number of reproduction is also limited (Edited by Zhao Shiwei , Landscape Design of Garden Engineering: Encyclopedia of Plant Configuration and Cultivation Application (Volume 1, 2 and 2), China Agricultural Science and Technology Press, 1st Edition, September 2000, p. 1457)

Method used

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  • Culturing method of momordica cochinchinensis

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Effect test

Embodiment 1

[0044] (1) Selection and disinfection of explants: Take the young shoots of the wood turtle as explants, soak them in 2% aqueous solution of detergent for 10 minutes, wash them with linear tap water for 15 minutes, add 2 drops of Tween-20 to 100 ml of 0.1 % mercuric chloride disinfection for 9 minutes, rinsed with sterile water for 4 times, and finally absorb the surface moisture with sterile filter paper to obtain explants, wherein the sterile water is distilled water sterilized by high pressure.

[0045] (2) Lateral bud differentiation to obtain clustered buds and rapid propagation: the explants obtained in step (1) were inoculated into MS medium, and cultivated for 30 days at a culture temperature of 24.5 °C, a light intensity of 1700 lux, and a light time of 11 h / d. Day, a large number of clustered shoots were obtained after lateral bud differentiation, wherein 0.2mg / L KT, 2.0mg / L 6-BA and 1.0mg / L NAA, 30g / L sucrose and 5.0g / L agar were added to the MS medium, and the mediu...

Embodiment 2

[0050] The invention provides a method for cultivating wood softshell turtles, comprising:

[0051] (1) Acquisition of wood turtle explants: Take wood turtle tender shoots as explants, first use 2% volume percentage of detergent aqueous solution to soak for 15 minutes, then use tap water to rinse for 20 minutes, and then use 0.1% mass volume fraction of detergent solution containing Sterilized with mercuric chloride at temperature -20 for 8 minutes, and finally rinsed with sterile water for 5 times to remove surface moisture to obtain wood softshell turtle explants.

[0052] (2) Differentiation culture: inoculate the explants of the wood turtle into the MS differentiation medium and cultivate them for 25 days until the lateral buds of the explants differentiate into clustered buds, wherein the culture temperature in the differentiation culture is 26°C, and the light intensity is 2000lux , the light cycle is 12 hours / day; MS differentiation medium contains: MS, 0.4mg / LKT, 2.0mg...

Embodiment 3

[0057] The invention provides a method for cultivating wood softshell turtles, comprising:

[0058] (1) Acquisition of wood turtle explants: Take wood turtle tender shoots as explants, first use 2% volume percentage of detergent aqueous solution to soak for 5 minutes, then use tap water to rinse for 10 minutes, and then use 0.1% mass volume fraction of detergent solution containing Sterilized with mercuric chloride at temperature -20 for 10 minutes, and finally rinsed with sterile water for 3 times to remove surface moisture to obtain wood softshell turtle explants.

[0059] (2) Differentiation culture: inoculate the explants of the wood turtle into the MS differentiation medium and cultivate them for 35 days until the lateral buds of the explants differentiate into clustered buds, wherein the culture temperature in the differentiation culture is 23°C, and the light intensity is 1400lux , the light cycle is 10 hours / day; MS differentiation medium contains: MS, 0.1mg / LKT, 1.0mg...

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Abstract

The invention discloses a culturing method of momordica cochinchinensis. The culturing method comprises the following steps: 1, differentiation culture, namely, inoculating a momordica cochinchinensis explant into an MS differentiation culture medium for 25-35 days until lateral buds of the explant is differentiated, wherein the culturing temperature is 23-26 DEG C, the illumination intensity is 1400-2000lux, and the illumination period is 10-12h/day; 2, strong seedling culture, namely, culturing the momordica cochinchinensis explant in an MS strong seedling culture medium for 35-45 days to obtain a momordica cochinchinensis strong plant, wherein the culturing temperature is 23-26 DEG C, the illumination intensity is 1400-2000lux, and the illumination period is 10-12h/day; and 3, rooting culture, namely, culturing the momordica cochinchinensis strong plant in an MS strong seedling culture medium for 35-45 days to obtain a rooted momordica cochinchinensis plant, wherein the culturing temperature is 23-26 DEG C, the illumination intensity is 1400-2000lux, and the illumination period is 10-12h/day. According to the method disclosed by the invention, a large number of momordica cochinchinensis seedlings suitable for being transplanted can be immediately propagated.

Description

technical field [0001] The invention relates to a method for plant propagation, in particular to a method for cultivating wood turtles. Background technique [0002] Wood turtle (Momordica cochinchinensis (Lour) Spreng) is a dioecious perennial herbaceous vine belonging to the family Cucurbitaceae, which has important ornamental and medicinal values. With saponins, orcitonin, sterols, alkaloids, etc. as the main active ingredients, it has broad development and application prospects in surgical medicine, insecticidal pesticides, etc. Scorpion is mainly distributed in Guangxi, Sichuan, Guangdong, Jiangxi, Hunan, Hubei, Yunnan, Guizhou and other provinces in China, and it is cultivated in most provinces and regions in the south (Chen Xiaojun, Cultivation Technology of Scorpion, Southern Horticulture, 2010, 03: 54 -55.). [0003] Wood softshell turtles are often propagated by seeds and root tubers; however, the number of seeds is limited, the germination and seedling rate is l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李刚王晓峰周琼缪剑华韦坤华
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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