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Tetrahydropyrimidine extraction process

An ectoine and extraction process technology, applied in the field of ectoine extraction process, can solve the problems of troublesome cleaning, damage to membrane life, high salt content, etc., and achieve the effect of simplifying the production process and reducing the processing load

Active Publication Date: 2015-04-29
SHANDONG FUTASTE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction of ectoine from this bacteria is generally done by ethanol extraction, but this method is time-consuming and laborious, and it is not easy to be used in mass production
[0003] Patent application number: 201110097221.1, the patent document titled "Membrane Technology Extraction Method for Ectoline and Hydroxyectoine" discloses a method for simply using membranes to extract ectoine. Although this method is simple in process, it is The seed solution has a high salt content, and the solution contains a certain amount of protein, colloid, etc. These substances are easy to block the membrane when it is treated with a membrane in the early stage, which will bring some troubles to the cleaning of the membrane. In severe cases, it will damage the life of the membrane. And only adopt membrane to extract ectoine substance, make the purity of ectoine substance be subject to certain restriction, can not obtain high-purity ectoine solution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Pretreatment: Take 15L of the bacterial cell solution containing ectoine, put it into a high-powered centrifuge, place the obtained bacterial cell in a dryer at 80°C for drying, and crush the dried bacterial cell with a pulverizer to Below 200 meshes, 1000 g of pulverized bacterial cells were obtained.

[0021] (2) Dissolution: Dissolve the pulverized bacteria in high-purity water according to the solid-to-liquid ratio of 1:10, stir fully to dissolve the ectoine substance, let it stand still, and use a filter to remove the floating bacteria fragments in the upper layer to obtain tetrahydropyrimidine. Solutions of Hydropyrimidine Substances.

[0022] (3) Decolorization: Add 2% of the solution obtained in (2) to powdered activated carbon for decolorization. The decolorization temperature is 50°C and the decolorization time is 30 minutes to obtain a filtered decolorization solution. The light transmission rate of the decolorization solution is 91.8%.

[0023] (4) Elec...

Embodiment 2

[0028] (1) Pretreatment: Take 15L of the bacterial cell solution containing ectoine, put it into a high-powered centrifuge, place the obtained bacterial cell in a dryer at 80°C for drying, and crush the dried bacterial cell with a pulverizer to Below 200 meshes, 1000 g of pulverized bacterial cells were obtained.

[0029] (2) Dissolution: Dissolve the pulverized bacteria in high-purity water at a solid-to-liquid ratio of 1:8, stir fully to dissolve the ectoine substance, let it stand, and filter out the floating bacteria fragments on the upper layer with a filter cloth to obtain tetrahydropyrimidine. Solutions of Hydropyrimidine Substances.

[0030] (3) Decolorization: Coat the solution obtained in the above (2) with 25% diatomaceous earth solution and filter it, add powdered activated carbon to the filtrate according to 1.5% of the solution mass for decolorization, decolorize at 60°C, and take 20 minutes to obtain a filtered decolorized solution. The light transmittance of t...

Embodiment 3

[0036] (1) Pretreatment: Take 15L of the bacterial cell solution containing ectoine, put it into a high-powered centrifuge, place the obtained bacterial cell in a dryer at 80°C for drying, and crush the dried bacterial cell with a pulverizer to Below 200 meshes, 1000 g of pulverized bacterial cells were obtained.

[0037] (2) Dissolution: Dissolve the pulverized bacteria in high-purity water at a solid-to-liquid ratio of 1:15, stir fully to dissolve the ectoine substance, let it stand, and use a filter to remove the floating bacteria fragments in the upper layer to obtain tetrahydropyrimidine. Solutions of Hydropyrimidine Substances.

[0038] (3) Decolorization: Add 3% of the solution obtained in the above (2) to powdered activated carbon for decolorization. The decolorization temperature is 60°C, the decolorization time is 30 minutes, and the diatomaceous earth coating is filtered to obtain a filtered decolorization solution. The decolorization solution has a light transmissi...

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PUM

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Abstract

The invention provides a tetrahydropyrimidine extraction process. The tetrahydropyrimidine extraction process mainly comprises the steps of pretreatment, tetrahydropyrimidine dissolution, decolorization, electrodialysis desalination, concentration, ion exchange, concentration, freezing crystallization and the like. As the cell disruption pretreatment method is adopted, tetrahydropyrimidine can be dissolved out to a great extent; as activated carbon is used for decolorization and ion exchange resin is used for purification, most proteins, colloids and other impurities in the solution can be removed while pigments are removed through decolorization by activated carbon, and accordingly, the follow-up treatment burden is relieved to a great extent; and only through the one-step electrodialysis desalination process, high-purity tetrahydropyrimidine crystals can be obtained and the production technology is greatly simplified.

Description

technical field [0001] The invention belongs to the field of chemical industry, and in particular relates to a process for extracting ectoine. Background technique [0002] Tetrahydropyrimidine is an osmotically compensating solute widely found in a moderately halophilic bacterium with the chemical name (s)-2-methyl-1,4,5,6-tetrahydromethylpyrimidine -4-Carboxylic acid, formula C 6 h 10 N 2 o 2 , molecular weight 142.16, accumulation in cells can balance the osmotic pressure of cells, and provide protection for enzymes, DNA, cell membranes and the whole cell in high temperature, dry, freezing and other environments, used in cosmetics, and play an immune role on pancreatic islet cells , can produce thermal protein, protect the integrity of the film, and reduce skin sunburn caused by ultraviolet radiation. The current global supply is about 10 tons, mainly in Germany, the price is about 30,000 / kg, and the output value is about 300 million yuan. The market prospect very br...

Claims

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Application Information

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IPC IPC(8): C07D239/06
CPCC07D239/06
Inventor 孙鲁邱学良曹玉华王关斌李毅邵竞峰梁学超周娟黄伟红姜希生
Owner SHANDONG FUTASTE
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