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Device and method for extracting and detecting compound in complicated system

A detection device and compound technology, which are applied in the fields of chemiluminescence/bioluminescence, preparation of test samples, and analysis by chemical reaction of materials, etc., can solve problems such as poor anti-interference ability, and achieve easy assembly, adjustment and selection. The effect of good performance and saving analysis and detection time

Active Publication Date: 2015-04-29
黑龙江联生生物科技有限公司
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Problems solved by technology

However, ECL itself has poor anti-interference ability, and the actual sample must be effectively pretreated before it can be used for quantitative analysis, which brings great inconvenience to research users
[0004] Therefore, for the current devices and methods for detecting compounds in complex systems using solid-phase microextraction and electrochemiluminescence, there is an urgent need for improvement; and there is no report on the combined use of solid-phase microextraction and electrochemiluminescence detection in the prior art

Method used

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  • Device and method for extracting and detecting compound in complicated system

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Embodiment 1

[0035] like figure 1 As shown, the extraction and detection device of the compound in the complex system of this embodiment includes a working electrode, an auxiliary electrode, a reference electrode, an extraction cell, a high-voltage DC power supply, an ultra-weak luminescence analyzer and an electrochemical workstation, and the working electrode is a glassy carbon electrode. , the surface of the glassy carbon electrode has a solid-state micro-extraction film, the solid-state micro-extraction film is graphene modified by functional groups; the auxiliary electrode is a platinum wire electrode; the reference electrode is an Ag / AgCl electrode; the extraction cell is used to hold Extraction solution; the two poles of the high-voltage DC power supply are respectively connected to the working electrode and the auxiliary electrode, and provide a voltage between -300V and +300V for the working electrode and the auxiliary electrode; the ultra-weak luminescence analyzer is connected to...

Embodiment 2

[0049] The extraction and detection device of this embodiment is the same as that of Embodiment 1, only the solid-state micro-extraction membrane on the surface of the working electrode is different.

[0050] In this embodiment, the method for extracting and detecting compounds in a complex system using the above-mentioned device includes the following steps:

[0051] (1) Preparation of the working electrode:

[0052] Graphene oxide GO was synthesized, and GO was chemically modified to obtain functionalized graphene Functionalized–G. Specifically, 50 mg NaOH and 50 mg sodium chloroacetate were added to 1 mL of GO suspension with a concentration of 1 g / L, and ultrasonically treated in a water bath at 30 ° C for 2 h to obtain a clear solution; the clear solution was first neutralized with dilute HCl, and then Rinse with deionized water repeatedly until neutral, then centrifuge until the product is well dispersed in deionized water, and finally put it in a 45°C oven to dry to ob...

Embodiment 3

[0061] The extraction and detection device of this embodiment is the same as that of Embodiment 1, only the solid-state micro-extraction membrane on the surface of the working electrode is different.

[0062] In this embodiment, the method for extracting and detecting compounds in a complex system using the above-mentioned device includes the following steps:

[0063] (1) Preparation of the working electrode:

[0064] Graphene oxide GO was synthesized, and GO was chemically modified to obtain functionalized graphene Functionalized–G. Specifically, 75 mg of GO was dissolved in 75 mL of water and ultrasonically treated for 1 h to obtain a brown homogeneous liquid; 600 mg of sodium borohydride was dissolved in 15 mL of water and added to the above brown homogeneous liquid, and the pH was adjusted to 9-9 with 5% wt of sodium carbonate solution. 10. Filter, wash the precipitate with deionized water until the filtrate pH=7, then dissolve the precipitate in water to obtain reduced g...

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Abstract

The invention relates to a device for extracting and detecting a compound in a complicated system. The invention further relates to a method for extracting and detecting the compound in the complicated system employing the device. Compared with the prior art, the invention has the advantages that functionalized graphene has a unique nano structure, so that a work electrode has good adsorption capability; luminol can be subjected to electrochemical reaction on the surface of graphene to shine by the conductive capability of the graphene, so as to achieve ECL determination; extraction and electrochemical testing are integrated on overall equipment; a target object is directly detected by an electrochemical luminescence instrument after the extraction process is finished; the elution step in a traditional analysis process is avoided; the analysis detection time is greatly shortened; and the detection efficiency is improved. The device disclosed by the invention is easy to assemble and adjust, and convenient to operate; and according to the method disclosed by the invention, the steps are simple, and flexibility and automatic degree are high, and the method is especially suitable for extraction and detection of ionic compounds and strong polar compounds in the complicated system.

Description

technical field [0001] The invention relates to the technical field of environmental chemistry and analytical chemistry detection, and specifically refers to a device for extracting and detecting compounds in a complex system. The invention also relates to a method for using the device to extract and detect compounds in a complex system. Background technique [0002] Sample pretreatment is an important step in the analysis of complex samples. Through sample pretreatment, interferences in the sample can be removed and the target can be concentrated to make up for the lack of existing separation techniques and instrument detection sensitivity. The steps of sample pretreatment mainly include sampling, dissolution, filtration, digestion, extraction, etc., among which extraction is the most important step. Solid phase microextraction (SPME) is a relatively new sample pretreatment technology developed on the basis of solid phase extraction. Compared with traditional sample pretrea...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N1/34G01N1/40
Inventor 吴文文王邃王欢李婷婷商芳
Owner 黑龙江联生生物科技有限公司
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