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A kind of determination method of scopolide, umbelliferone and osthole content in Fuqing Xuelian Suppository

A technology of umbelliferone and Fuqing snow lotus suppository, which is applied in the field of pharmaceuticals, can solve problems that have not been reported, and achieve the effect of strong operability, high accuracy and simple method

Active Publication Date: 2016-05-18
GUANGZHOU HANFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the content determination methods of scopoletin and umbelliferone in medicinal materials and preparations have been reported in the literature, and there are also literature reports on the determination of Cnidium chinensis in the medicinal materials Cnidium Fructus, Duhuo and the traditional Chinese medicine preparation Fuyanling Capsules. However, the method for simultaneously determining the content of these three components in traditional Chinese medicine preparations has not been reported.

Method used

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  • A kind of determination method of scopolide, umbelliferone and osthole content in Fuqing Xuelian Suppository
  • A kind of determination method of scopolide, umbelliferone and osthole content in Fuqing Xuelian Suppository
  • A kind of determination method of scopolide, umbelliferone and osthole content in Fuqing Xuelian Suppository

Examples

Experimental program
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Effect test

Embodiment 1

[0020] (1) Preparation of test solution

[0021] Take Fuqing Snow Lotus Suppository as raw material, take 10 capsules of Fuqing Snow Lotus Suppository, heat and melt in a water bath at 60°C, and let cool. Take about 1.5g, cut it into pieces, weigh it accurately, put it in a stoppered Erlenmeyer flask, add 15ml, 15ml, and 15ml of methanol respectively, ultrasonicate (power 360W, frequency 35kHz) 3 times, each time for 20 minutes, filter, and combine Put the filtrate in a 50ml measuring bottle, add methanol to the mark, shake well, and it is the test solution;

[0022] (2) Content determination of scopoletin, umbelliferone and osthole

[0023] The experimental conditions of high performance liquid chromatography are as follows: C 18 Chromatographic column: 250mm×4.6mm, 5μm; mobile phase: acetonitrile as mobile phase A, water as mobile phase B; elution gradient: 0-17min: 10%-60%A; 17-18min: 60%-80 %A; 18-24min: 80%A; 24-30min: 80%-95%A; 30-32min: 95%-10%A; 32-42min: 10%A. Elu...

Embodiment 2

[0027] (1) Preparation of test solution

[0028] Take Fuqing Snow Lotus Suppository as raw material, take 10 capsules of Fuqing Snow Lotus Suppository, heat and melt in a water bath at 60°C, and let cool. Take about 1.5g, cut it into pieces, weigh it accurately, put it in a stoppered Erlenmeyer flask, add 15ml, 15ml, and 15ml of methanol respectively, ultrasonicate (power 360W, frequency 35kHz) 3 times, each time for 20 minutes, filter, and combine Put the filtrate in a 50ml measuring bottle, add methanol to the mark, shake well, and it is the test solution;

[0029] (2) Content determination of scopoletin, umbelliferone and osthole

[0030] The experimental conditions of high performance liquid chromatography are as follows: C 18 Chromatographic column: 250mm×4.6mm, 5μm; mobile phase: acetonitrile as mobile phase A, 0.1% glacial acetic acid water as mobile phase B; elution gradient: 0-8min: 15%-30%A; 8-23min: 30-35%A; 23-43min: 35%-95%A; Elution time: 43min; UV detector de...

Embodiment 3

[0034] (1) Preparation of test solution

[0035] Take Fuqing Snow Lotus Suppository as raw material, take 10 capsules of Fuqing Snow Lotus Suppository, heat and melt in a water bath at 60°C, and let cool. Take about 1.5g, cut it into pieces, weigh it accurately, put it in a stoppered Erlenmeyer flask, add 15ml, 15ml, and 15ml of methanol respectively, ultrasonicate (power 360W, frequency 35kHz) 3 times, each time for 20 minutes, filter, and combine Put the filtrate in a 50ml measuring bottle, add methanol to the mark, shake well, and it is the test solution;

[0036] (2) Content determination of scopoletin, umbelliferone and osthole

[0037] The experimental conditions of high performance liquid chromatography are as follows: C 18 Chromatographic column: 250mm×4.6mm, 5μm; mobile phase: acetonitrile as mobile phase A, 0.1% glacial acetic acid water as mobile phase B; elution gradient: 0-5min: 15%-32%A; 5-20min: 32%A; 20-21min: 32%-75%A; 21-40min: 75%-90%A; 40-45min: 90%-95%A...

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Abstract

The invention discloses a method for measuring content of scopoletin, umbelliferone and osthole in Fuqing saussurea involucrata suppository. The method comprises steps as follows: (1), preparation of a solution for test articles; (2) measurement of content of scopoletin, umbelliferone and osthole ingredients: content of three ingredients of scopoletin, umbelliferone and osthole is measured with a high performance liquid chromatography technology, peak areas of scopoletin, umbelliferone and osthole chromatographic peaks are obtained, and the content of three ingredients of scopoletin, umbelliferone and osthole in the test articles is calculated according to a standard curve; in combination of the mass of the test articles, after calculation, the content of scopoletin is is 0.569 mg / g-0.592 mg / g, the content of umbelliferone is 1.034 mg / g-1.068 mg / g, and the content of osthole is 8.65mg / g-8.89mg / g. The method can be used for evaluating the quality of the Fuqing saussurea involucrata suppository; a quality detecting method is accurate, fast, convenient and effective.

Description

technical field [0001] The invention belongs to the field of medicines, and in particular relates to a content determination method for three active ingredients in Fuqing Snow Lotus Suppository. Background technique [0002] Fuqing Snow Lotus Suppository is a new compound traditional Chinese medicine independently developed by our company. It is used to treat gynecological extravaginal inflammation. , cold, into the liver, spleen, kidney meridian, has the effect of clearing heat and detoxification, reducing swelling and relieving pain, and is used for head trauma, anthrax, thermal tingling, gynecological diseases, rheumatoid arthritis, stroke and other diseases. Among them, scopoletin and umbelliferone are active ingredients in snow lotus. Fructus Cnidii is the dried and mature fruit Cnidium Fructus Cnidii of Umbelliferae plant Cnidium, and its medicinal properties are pungent, bitter, warm, slightly poisonous, and return to the kidney meridian. It has the functions of dry...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 王小妹刘春芳蒋兆健李菁林秀仙袁诚李金华刘洁瑜葛发欢
Owner GUANGZHOU HANFANG PHARMA