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Method for improving wheat drought resistance and salt stress resistance by taking sodium hydrosulfide as hydrogen sulfide donor

A technology of hydrogen sulfide donor and sodium hydrosulfide, which is applied in botany equipment and methods, seed and rhizome treatment, chemicals for biological control, etc., can solve the problems of extremely poor growth, achieve growth promotion, improve resistance Inverse, damage-avoiding effects

Active Publication Date: 2015-05-06
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wheat, as a salt-drought sensitive plant, grows extremely poorly in salinized and dry soils

Method used

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  • Method for improving wheat drought resistance and salt stress resistance by taking sodium hydrosulfide as hydrogen sulfide donor
  • Method for improving wheat drought resistance and salt stress resistance by taking sodium hydrosulfide as hydrogen sulfide donor
  • Method for improving wheat drought resistance and salt stress resistance by taking sodium hydrosulfide as hydrogen sulfide donor

Examples

Experimental program
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Effect test

Embodiment 1

[0023] A method of using sodium hydrogen sulfide as a hydrogen sulfide donor to improve wheat drought resistance and salt stress resistance. The application of sodium hydrogen sulfide as a hydrogen sulfide donor to improve the stress-resistant germination of wheat seeds in drought and salt stress environments is that wheat seeds are at a concentration of Soak in 0.03~1.2mmol / L sodium hydrosulfide aqueous solution at room temperature for 12~24h.

[0024] Specific steps are as follows:

[0025] (1) Weigh 0.56g of sodium hydrosulfide and dissolve it in deionized water, dilute it into a 1000mL volumetric flask, and prepare mother solution A of 10mmol / L NaHS with a pH value of 9;

[0026] (2) Take different volumes of the mother liquor A in step (1), add deionized water according to the proportion, and dilute it into sodium hydrosulfide aqueous solution with different concentrations between 0 and 1.2mmol / L;

[0027] (3) Select wheat seeds with plump grains, uniform size, and moi...

Embodiment 2

[0033] Concrete steps are with embodiment 1.

[0034] Germination test under drought stress was carried out by adopting the seeds of wheat variety Aikang 58, and the soaked seeds were transferred to a petri dish, with 40 wheat seeds in each petri dish, repeated three times; then adding mass percentage in each petri dish The concentration of 10% PEG-6000 solution was used to simulate drought stress treatment, and the PEG-6000 solution was replaced every 12 hours to observe the germination of wheat seeds, and the germination rate of wheat was counted after 12 hours and 3 days after germination. As shown in table 2.

[0035] Table 2 Effects of soaking seeds in different concentrations of sodium hydrosulfide aqueous solution on the germination rate of Aikang 58 seeds under drought stress

[0036]

[0037] As can be seen from Table 2, the germination rate of wheat was counted at 12h and 72h. It can be seen that applying sodium hydrosulfide under drought stress can significa...

Embodiment 3

[0039] Concrete steps are with embodiment 1.

[0040] Using the germination test of wheat variety Yumai 49-198 seeds under salt stress, the soaked seeds were transferred to a petri dish, 40 wheat seeds in each petri dish, repeated three times; then 0.12mol / L sodium chloride salt solution for salt stress treatment, replace the sodium chloride salt solution every 12h, observe the germination of wheat seeds, count the germination rate of wheat and the shoot length and root length after 6 days after 12h and 3 days after germination. as shown in Table 3.

[0041]Table 3 Effects of soaking seeds in different concentrations of sodium hydrosulfide aqueous solution on the germination rate of Yumai 49-198 seeds under salt stress

[0042]

[0043] It can be seen from Table 3 that different concentrations of sodium hydrosulfide can improve the salt resistance of wheat seeds. From the comparison of germination rate, shoot length and root length, it can be seen that applying sodium ...

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Abstract

The invention discloses a method for improving wheat drought resistance and salt stress resistance by taking sodium hydrosulfide as a hydrogen sulfide donor. The method concretely comprises the steps of before sowing, soaking wheat seeds into a sodium hydrosulfide water solution with the concentration of 0.03-1.2mmol / L for 12-24 hours under the condition of room temperature, or carrying out root soaking, root-irrigation or foliage spray treatment on wheat seedlings at the seedling stage by using a sodium hydrosulfide mixed water solution with the concentration of 0.01-0.6mmol / L under the condition of room temperature; and at the later period of wheat growth, spraying a sodium hydrosulfide mixed solution with the concentration of 0.05-0.8mmol / L onto the leaf surfaces of the wheat plants. According to the method, different sodium hydrosulfide application ways and concentrations are adopted according to the different growth stages of the wheat plants, so that the substances related with stress resistance in the plants can be increased, and the stress resistance of the plants can be improved.

Description

technical field [0001] The invention belongs to the technical field of wheat cultivation, and in particular relates to a method for improving wheat drought resistance and salt stress resistance by using sodium hydrosulfide as a hydrogen sulfide donor. Background technique [0002] Abiotic stresses such as drought, salinity, and extreme temperature are the primary reasons for global food crop production decline. Wheat, as a salt-drought sensitive plant, grows extremely poorly in salinized and dry soils. my country's water resources are seriously scarce, and drought and water shortage have become the main limiting factors of my country's grain production. National salinized land accounts for about 10% of the country's land area, and with global warming, shortage of fresh water resources and continuous increase in population, this phenomenon is still intensifying. Therefore, studying the physiological function and stress tolerance mechanism of wheat under drought and salt str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01N59/02A01P21/00
Inventor 马冬云丁会纳秦海霞王晨阳郭天财
Owner HENAN AGRICULTURAL UNIVERSITY
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