Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Agrobacterium rhizogenes mediated strawberry gene transferring method

A technology of Agrobacterium rhizogenes and transgene, which is applied in genetic engineering, plant genetic improvement, botany equipment and methods, etc., to achieve high transformation efficiency and save research time

Inactive Publication Date: 2015-05-06
BEIJING UNIV OF AGRI
View PDF4 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, strawberry transgenic has not been reported. Therefore, it is necessary to establish a set of Agrobacterium-mediated, efficient and stable transgenic technology for the genetic improvement, gene mining and functional analysis of strawberry.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Agrobacterium rhizogenes mediated strawberry gene transferring method
  • Agrobacterium rhizogenes mediated strawberry gene transferring method
  • Agrobacterium rhizogenes mediated strawberry gene transferring method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] Now in conjunction with specific embodiment, each step of the present invention is further described in detail:

[0024] 1. The expression vector MSU440 Agrobacterium rhizogenes containing 35S::DsRed reporter system: donated by the research group of Ton Bisseling of Wageningen University.

[0025] 2, forest strawberry seeds, cold treatment, 4 ℃ refrigerator storage for 7 days; seed disinfection: 75% ethanol treatment 5min, 1% sodium hypochlorite NaClO treatment 5min, sterile distilled water rinse 2-3 times; inoculation on MS base, dark culture 10 days.

[0026] 3. Activation of Agrobacterium rhizogenes: Streak Agrobacterium rhizogenes on LB solid medium containing 50 mg / L spectinomycin, culture upside down in the dark at 28°C for 48-72 hours to obtain a single colony, pick a single colony in On fresh LB liquid medium containing 50mg / L spectinomycin (spectinomycin), culture in dark at 28°C and 220rpm for 48h. Aspirate the bacterial solution and apply it again on LB sol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an Agrobacterium rhizogenes mediated strawberry gene transferring method. Agrobacterium rhizogenes MSU440 containing 35S::DsRed reporter gene is utilized to infect forest strawberry aseptic seedling hypocotyl to obtain a co-transformation plant, so as to obtain a transformed hairy root. According to the proposed forest strawberry hypocotyl transformation method, the transformation period which is one and half months can be shortened greatly, and the experimental result is also intuitive; an in-vitro strawberry hairy root system can provide a rapid and reliable method for strawberry gene function identification.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to plant transgenic technology, and mainly relates to a strawberry transgenic method mediated by Agrobacterium rhizogenes. Background technique [0002] Strawberry is a perennial herbaceous fruit tree of the Rosaceae (Rosaccae) genus Fragaria. At present, domestic research on strawberry is mostly focused on physiological and biochemical aspects, while there are few reports on gene mining and functional analysis; secondly, there are more researches on strawberry fruit, such as real coloring, but less research on strawberry root system. In addition, the forest strawberry (Fragaria vesca) genome has been sequenced and gene annotation has been performed, which is of great significance for mining the functional genes controlling important traits. At present, the lack of a stable and efficient transgenic system has become a bottleneck restricting the research of strawberry molecular biology. [0...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/84A01H5/06
Inventor 邢宇曹庆芹赵晨晨秦岭张卿杨柳
Owner BEIJING UNIV OF AGRI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com