Mo Hsp70 protein based indirect ELISA (enzyme-linked immunosorbent assay) kit and using method thereof

Abstract

The invention discloses a Mo Hsp70 protein based indirect ELISA (enzyme-linked immunosorbent assay) kit and a using method of the Mo Hsp70 protein based indirect ELISA kit. The Mo Hsp70 protein based indirect ELISA kit is prepared from the following raw materials: a Mo Hsp70 protein coated ELISA plate, Mo standard positive serum, Mo standard negative serum, an enzyme-labeled secondary antibody, 50-time PBST buffer solution, block buffer solution, substrate solution A, substrate solution B and stop buffer. The Mo Hsp70 protein based indirect ELISA kit can detect the Mo Hsp70 protein antibody of goat serum, can provide key technology for the early monitoring of interstitial pneumonia of sheep and goats, caused by Mo, the immune effect evaluation of vaccines and correlational research, and has important significance on early detection and early control of epidemic diseases caused by the pathogen.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Mo Hsp70 protein-based indirect ELISA kit and a use method. Background technique [0002] Mycoplasma ovis pneumoniae ( Mycoplasma ovipneumoniae, Mo) is a pathogen that causes interstitial pneumonia (atypical pneumonia) in goats and sheep, which can cause cough, runny nose, progressive emaciation and proliferative interstitial pneumonia symptoms in goats and sheep (Zhang Shuangxiang et al., 2013; Ran Shuangcun, 2010 ; Han Xiao, 2013). In 1963, Mo was first isolated from the lung tissue of Scotland-onset sheep disease by Mackay et al. (Mackay et al., 1963), Spain, Australia, New Zealand, Hungary, Iceland, the United Kingdom, and many countries in Africa have successively reported that Mo causes sheep and goat diseases. occurrence (Xu Jian et al., 2012). Since the first discovery of the pathogen in breeding sheep from New Zealand in 1978 in my country (Hu Jingshao et al., 1982), re...

AI Technical Summary

Problems solved by technology

[0003] The prevention and control of Mycoplasma pneumoniae in sheep currently mainly relies on drug prevention, and there is still a lack of reliable vaccines. The reason is related to the time-consuming and laborious isolation and cultivation of Mo and the slow genome research (Hou Xiangshan et al., 2006; Zhao Ping et al., 2008)

Moreover, there is currently a lack of rapid serological detection methods required for disease detection and prevention and control, especially the ELISA method with high throughput and high sensitivity

Images