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Application of cynanchum ascyrifolium extract in preparation of tumor cell multidrug resistance reversal agents

A technology for multi-drug resistance and tumor cells, which is applied in the field of medicine to achieve the effects of low energy consumption, easy mastery of the technology, and wide application prospects

Inactive Publication Date: 2015-05-13
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is used for internal heat due to yin deficiency, hot flashes due to bone steaming, spontaneous sweating and night sweating, wind-temperature scorching heat and insomnia, postpartum deficiency and troublesome blood syncope, lung-heat hemoptysis, febrile malaria, hot stranguria, bloody stranguria, wind-warm arthralgia, scrofula, sore throat Swelling and pain, mastitis, sore carbuncle and swelling and pain, but so far there is no report on the use of Chaofengcao extract as a reversal agent for tumor cell multidrug resistance

Method used

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  • Application of cynanchum ascyrifolium extract in preparation of tumor cell multidrug resistance reversal agents
  • Application of cynanchum ascyrifolium extract in preparation of tumor cell multidrug resistance reversal agents
  • Application of cynanchum ascyrifolium extract in preparation of tumor cell multidrug resistance reversal agents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation of Chaofengcao Extract

[0032] After crushing 10 kg of Chaofengcao whole herb, add 90% ethanol to reflux and extract three times, 80 liters each time, each extraction time is 2 hours, filter in several times, combine the extracts, and use the processed decolorizing resin D941 column chromatography , filled with decolorizing resin D941 with a volume of 5 liters, eluted with 30 liters of 90% ethanol, collected the eluate, concentrated under reduced pressure, and dried to obtain 65 grams of Chaofengcao extract.

Embodiment 2

[0034] Chaofengcao extract reverses the drug resistance of human gastric cancer multidrug-resistant cell line SGC7901 / VCR to doxorubicin

[0035] Take the multidrug-resistant human gastric cancer cell line SGC7901 / VCR in the logarithmic growth phase and adjust the cell concentration to 1×10 with PPMI-1640 medium containing 10% FBS. 5 cells / mL, seeded in 96-well cell culture plate, 100uL / well, placed in 5% CO 2 Cultured in a constant temperature incubator at 37°C for 24 hours, and then added 100 μL of culture medium containing different concentrations of doxorubicin, tidal grass extract (final concentration 50 μg / ml) and culture medium of different concentrations of doxorubicin to each well, In the culture medium of Chaofengcao extract (final concentration 25 μg / ml) and different concentrations of doxorubicin, after adding the drug, continue to cultivate for 48 hours, add 20 μl of 5% MTT to each well, and continue to cultivate for 4 hours. Aspirate and discard the culture medi...

Embodiment 3

[0039] Chaofengcao extract reverses the drug resistance of human gastric cancer multidrug resistant cell line SGC7901 / VCR to 5-fluorouracil

[0040] Take the multidrug-resistant human gastric cancer cell line SGC7901 / VCR in the logarithmic growth phase and adjust the cell concentration to 1×10 with PPMI-1640 medium containing 10% FBS. 5 cells / mL, seeded in 96-well cell culture plate, 100uL / well, placed in 5% CO 2 Incubate in a constant temperature incubator at 37°C for 24 hours, and then add 100 μL of culture medium containing different concentrations of 5-fluorouracil, tidal grass extract (final concentration 50 μg / ml) and culture medium of different concentrations of 5-fluorouracil to each well, In the culture medium of Chaofengcao extract (final concentration 25 μg / ml) and different concentrations of 5-fluorouracil, after adding the medicine, continue to cultivate for 48 hours, add 20 μl of 5% MTT to each well, and continue to cultivate for 4 hours. Aspirate and discard th...

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Abstract

The invention discloses an application of a cynanchum ascyrifolium extract in the preparation of tumor cell multidrug resistance reversal agents. The cynanchum ascyrifolium extract reverses the drug-resistant actions of human gastric tumor multidrug resistance cells, human colonic carcinoma multidrug resistance cells and human lung carcinoma multidrug resistance cells on clinical commonly used drugs such as taxol, adriamycin, 5-fluorouracil and cis-platinum. The cynanchum ascyrifolium extract disclosed by the invention is simple and convenient in preparation method, is combined with antitumor drugs to be applied to clinical application and has wide application prospects, and the curative effect of chemotherapy is improved.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the application of the extract of Chaofengcao in the preparation of a tumor cell multidrug resistance reversal agent. Background technique [0002] Malignant tumor is one of the diseases that seriously endanger human health. It is characterized by abnormal cell proliferation and metastasis. Statistics from the World Health Organization show that in 2000, there were about 10 million new cases of malignant tumors in the world, 6.2 million deaths, and 22 million cases of illness; it is estimated that by 2020, there will be 15 million new cases of malignant tumors, 10 million deaths, and 30 million illnesses. Thousands of cases. Chemotherapy is one of the methods of comprehensive therapy for malignant tumors. It is widely used before, during and after surgery. However, the phenomenon of multidrug resistance of tumor cells seriously affects the curative effect of chemotherapy on malignant tumor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/27A61P35/00
Inventor 王威刘小红韩立春高华刘坤
Owner QINGDAO UNIV
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