A kind of cation exchange chimeric crystal gel separation medium and preparation method thereof

Abstract

The invention discloses a cation exchange chimeric cryogel separation medium and a preparation method thereof. The cation exchange chimeric cryogel separation medium is characterized in that the chimeric cryogel medium is obtained from grafting and modifying a poly hydroxyethyl methacrylate continuous bed medium with internally chimeric cellulose microspheres through 2-acrylamide-2-methyl-1-propanesulfonic acid, a pore diameter of the chimeric cryogel medium ranges from 0.1 mu m to 300 mu m, the porosity is 80%-95%, the chimeric cryogel medium has a sulfonic group cation exchange functional group, and the adsorption capacity of cryogel for lysozyme model protein is 1-5mg / mL. The pore diameter of the chimeric cryogel medium ranges from sub-micron dimension to micron dimension, the cryogel medium has multi-level pores including diffusion mass transfer small pores and convective mass transfer ultra-large pores, and accordingly, the specific surface area of the cryogel medium and adsorption sites after chemical modification are increased; meanwhile, a matrix material of the cryogel medium has good biocompatibility, is stable in a separation environment, can be repeatedly used, has good safety and has wide application prospect in the biochemical separation field.

Description

technical field [0001] The invention belongs to the technical field of chemical separation media, and in particular relates to a cation-exchange chimeric crystal colloid separation media and a preparation method thereof. Background technique [0002] Crystal gel medium is a new type of chromatographic separation medium that has appeared in recent years. Its medium skeleton has super large pores ranging in size from several microns to hundreds of microns, allowing microbial cells or cell fragments to pass through smoothly. Therefore, it is suitable for fermentation from microorganisms. Rapid separation of biomacromolecular substances in fluids, cell culture fluids, blood, and complex biological fluids containing other solid-phase impurities such as whey, milk, etc. The separation process has few steps, the mass transfer of biological macromolecules in the gel bed column is rapid, the separation time is short, and the separation efficiency is good, so it has important applicat...

AI Technical Summary

Problems solved by technology

[0003] Although scholars at home and abroad have successfully prepared different series of crystal colloidal media, the pore size of the existing crystal colloidal media is mainly concentrated in a few microns to about 100-200 microns, with small specific surface area, single pore level, and adsorption sites. Less, so that the adsorption capacity and separation effect of crystal gel media are not fully exerted

However, it is difficult to prepare a multi-level subporous crystal colloidal medium through the coupling method of conventional crystallization porosity and polymerization reaction.

At the same time, there are no reports in the literature on the crystal gel embedded composite crystal gel medium with cation exchange functional groups.