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Method for preparing special peanut cake powder for fermentation

A technology of peanut cake flour and peanut cake, which is applied in the direction of fermentation, etc., to achieve the effects of convenient operation, improved functional characteristics and easy automation

Inactive Publication Date: 2015-05-13
HENAN ZHENXIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a method for preparing special-purpose peanut cake powder for fermentation. The present invention fully combines enzymatic hydrolysis technology and screw extrusion technology to modify peanut cake powder, thereby preparing a special peanut cake powder for fermentation. Cake powder not only solves the demand for a comprehensive nitrogen source medium in industrial fermentation, but also meets the needs of microorganisms for normal metabolism and synthesis of products during industrial fermentation, and the special fermentation medium can provide nutrients for the formation of metabolites Suitable precursor substances, coenzymes or cofactors, etc., thereby providing improved metabolite yields

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Take peanut cake without mildew, crush it and pass it through a 40-mesh sieve, and take the sieve to obtain peanut cake powder. The peanut cake is produced by high-temperature oil extraction from non-transgenic peanuts, and its protein content mass fraction is greater than 46 %, the fat content mass fraction is greater than 6%,

[0027] 2) The peanut cake powder obtained in 1) is mixed with an industrial enzyme preparation, the industrial enzyme preparation is Alcalase enzyme, Protemax enzyme and Papain enzyme, the Alcalase enzyme dosage is 0.05% of the peanut cake powder weight, and the Protemax enzyme dosage is peanut cake powder 0.05% by weight, Papain enzyme dosage is 0.1% by weight of peanut cake powder, mixed uniformly and sent to twin-screw extrusion extruder, extruded and expanded, the temperature of the extruded expanded is 80 ℃, and the pressure is 0.8MPa.

[0028] 3) Drying by air flow 2) Squeeze the puffed raw material, and use dry air at 60°C to take awa...

Embodiment 2

[0032] 1) Take peanut cake without mildew, crush it and pass it through a 40-mesh sieve, and take the sieve to obtain peanut cake powder. The peanut cake is produced by high-temperature oil extraction from non-transgenic peanuts, and its protein content mass fraction is greater than 46 %, the fat content mass fraction is greater than 6%,

[0033] 2) The peanut cake powder obtained in 1) is mixed with an industrial enzyme preparation, the industrial enzyme preparation is Alcalase enzyme, Protemax enzyme and Papain enzyme, the Alcalase enzyme dosage is 0.1% of the peanut cake powder weight, and the Protemax enzyme dosage is peanut cake powder 0.1% by weight, the amount of Papain enzyme is 0.15% of the weight of peanut cake flour, mixed uniformly and then sent to a twin-screw extrusion extruder for extrusion and expansion. The temperature of the extrusion expansion is 120 ° C and the pressure is 1 MPa.

[0034] 3) Drying by airflow 2) Extruding the puffed raw material, using dry ...

Embodiment 3

[0038] 1) Take peanut cake without mildew, crush it and pass it through a 40-mesh sieve, and take the sieve to obtain peanut cake powder; the peanut cake is produced by high-temperature oil extraction from non-transgenic peanuts, and its protein content mass fraction is greater than 46 %, the fat content mass fraction is greater than 6%,

[0039] 2) The peanut cake powder obtained in 1) is mixed with an industrial enzyme preparation, the industrial enzyme preparation is Alcalase enzyme, Protemax enzyme and Papain enzyme, the Alcalase enzyme dosage is 0.15% of the peanut cake powder weight, and the Protemax enzyme dosage is peanut cake powder 0.15% by weight, the amount of Papain enzyme is 0.2% of the weight of peanut cake powder, mixed uniformly and then sent to a twin-screw extruder for extruding and expanding. The temperature of the extruding and expanding is 150°C and the pressure is 1.5MPa

[0040] 3) Drying by air flow 2) Squeeze the puffed raw material, and use dry air a...

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Abstract

The invention relates to a method for preparing special peanut cake powder for fermentation. The method comprises the following steps of: taking non-mildew peanut cake, crushing, passing a 40-mesh mesh sieve, and taking sieved materials to obtain the peanut cake powder; mixing the peanut cake powder and an industrial enzyme preparation, conveying into a double-screw extruding and bulking machine after mixing uniformly, extruding and bulking; drying extruded and bulked materials, and enabling the water content of the materials to be less than 8-12%; and crushing the dried materials by a micro-powder machine while controlling the temperature to be below 80 DEG C in the crushing process to obtain the special peanut cake powder for fermentation. The method has the advantages that not only is the problem for the need of a nitrogen-source culture medium with comprehensive nutrition in industrial fermentation solved and is the need for normal metabolic and synthetic products of microorganisms in the industrial fermentation process met, but also the special culture medium for fermentation can also provide proper precursor substances, coenzymes or cofactors and the like for forming the metabolic products, so that the yield of metabolites is increased.

Description

technical field [0001] The invention relates to a method for preparing peanut cake flour specially used for fermentation, and belongs to the field of production and preparation methods of nitrogen source medium for industrial fermentation. Background technique [0002] At present, industrial fermentation, especially antibiotic fermentation such as streptomycin and erythromycin, mainly use peanut cake powder as the main nitrogen source of the medium. Traditional peanut cake powder is made by simply crushing peanut cake produced by high-temperature oil extraction. Due to the severe denaturation of the peanut protein contained in the high-temperature-pressed peanut cake, a large number of insoluble protein aggregates are formed, which leads to a decrease in its solubility, which in turn affects the emulsification, foaming, gelling, and nutritional properties of the peanut protein. How to develop a special peanut cake powder for fermentation with comprehensive nutritional compon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/00
Inventor 岳新建
Owner HENAN ZHENXIN BIOTECH CO LTD
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