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Detection method of vitamin in food

A food and biotin technology, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of unstable enzyme-linked immunosorbent assay results, high detection lower limit, long detection period, etc., to achieve good extraction effect, rapid analysis, The effect of a short analysis cycle

Active Publication Date: 2015-05-13
北京市营养源研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection limit of high performance liquid chromatography is too high, and the detection result of enzyme-linked immunoassay is not stable enough. Although the detection result of classical microbiological method is accurate and the detection limit is low, the detection cycle is long, the repeatability is poor, time-consuming and laborious, and it is harmful to the experimental operation environment. stricter requirements

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  • Detection method of vitamin in food
  • Detection method of vitamin in food
  • Detection method of vitamin in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0121] Determination of Biotin in Food by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

[0122] 1. The experimental equipment and reagents are as follows

[0123] 1) Instrument: Acquity ＠ UPLC-XevoTQ ultra-high performance liquid chromatography-tandem mass spectrometry (Waters, USA); chromatographic column: Acquity ＠ HSS T3 column (Waters, USA); high-speed refrigerated centrifuge (HITACHI, Japan); vortex shaker (Scientific Industries, USA); analytical balance (Sartorius, Germany), 0.22 μm GHP filter membrane (Pall, USA).

[0124] 2) Reagent: Biotin (VB 7 ) standard (Iso Sciences, USA), formic acid, ammonium acetate (Sigma, USA); ethanol, acetonitrile, chloroform (Fisher, USA); ultrapure water.

[0125] 2. The experimental design is as follows:

[0126] 1) In this embodiment, the best chromatographic conditions and mass spectrometry conditions are determined with biotin standards.

[0127] 2) In this example, determine the best sample pretreatment met...

Embodiment 2

[0162] Determination of Biotin in Food by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

[0163] 1. Experimental instruments and reagents are as follows:

[0164] 1) instrument is the same as embodiment one

[0165] 2) reagent is the same as embodiment one

[0166] 2. The experimental design is as follows:

[0167] 1) In this embodiment, the best chromatographic conditions and mass spectrometry conditions are determined with biotin standards.

[0168] 2) In this example, determine the best sample pretreatment method.

[0169] 3) Determining the sample to be tested by an established method to determine whether it contains biotin.

[0170] 4) Add a biotin standard substance to the sample to be tested, and determine the recovery rate, precision, and minimum detection limit of the method.

[0171] 3. The test method is as follows:

[0172] 1) Extraction of biotin in the sample

[0173] Weigh 0.5 g of infant formula milk powder sample into a 10 mL centrifu...

Embodiment 3

[0204] Simultaneous Determination of Biotin, Pantothenic Acid and Cyanocobalamin in Food by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

[0205] 1. The experimental equipment and reagents are as follows

[0206] 1) Instrument

[0207] Acquity ＠ UPLC-Xevo TQ ultra-high performance liquid chromatography-tandem mass spectrometry instrument, Waters Corporation of the United States; Acquity ＠ HSS T3 column, Waters Company of the United States; high-speed refrigerated centrifuge, HITACHI Company of Japan; vortex shaker, Scientific Industries Company of the United States; analytical balance, Sartorius Company of Germany; 0.22 μm GHP filter membrane (Pall Company of the United States).

[0208] 2) Reagent

[0209] Milk powder (commercial formula milk powder); pantothenic acid (VB 5 ), Biotin (VB 7 ), cyanocobalamin (VB 12 ) purity is all greater than 99.5%, U.S. IsoSciences company; Formic acid, ammonium acetate (all are chromatographically pure), U.S. sig...

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Abstract

The invention relates to a detection method of vitamin in food, in particular to a method for detecting biotin content in the food by using ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometer. The method comprises a sample pre-treatment method, ultra-high performance liquid chromatography detection, and tandem mass spectrometric detection step, and comprises the following steps: separating by using HSS T3 liquid chromatographic column, performing gradient eluting by using 10mmol / L ammonium acetate buffer solution (containing 0.1% formic acid) and acetonitrile as mobile phases, and performing qualitative and quantitative analysis by using electron spray-positive ion ionization source multi-reaction monitoring mode. The result is that the method quantitation limit of the biotin (VB7) is 0.6 microgram / 100g, the recovery rate is 81.3%, relative standard deviation is 5.5%, and the linear range is 0.2-10ng / mL. The method is simple in analysis operation, fast, high in sensitivity, good in repeatability, and suitable for quantitatively detecting the biotin in milk powder.

Description

technical field [0001] The invention relates to the field of detection and analysis of vitamin content in food, in particular to a method for detecting vitamin content in food by ultra-high performance liquid chromatography-tandem mass spectrometry. Background technique [0002] Vitamins are organic compounds necessary for life-maintaining physiological functions. They are contained in very small amounts in the natural ingredients of food. Although they are trace amounts, they are essential to the normal physiological functions of the matrix. If they are missing or insufficient, they will cause special deficiency syndromes. At present, 13 substances or substance groups are generally regarded as vitamins, which can be divided into fat-soluble vitamins and water-soluble vitamins according to their solubility. Methods for the determination of water-soluble vitamins include microbial methods, liquid chromatography, and high performance liquid chromatography-mass spectrometry. A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/08G01N30/14G01N30/36
Inventor 李菁菁叶润李全霞崔亚娟李东
Owner 北京市营养源研究所有限公司
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